Candida andamanensis sp. nov., Candida laemsonensis sp. nov. and Candida ranongensis sp. nov., anamorphic yeast species isolated from estuarine waters in a Thai mangrove forest

Abstract

Five strains (RV5T, RV140, R31T, RS17 and RS28T) representing three novel anamorphic ascomycetous yeast species were isolated by membrane filtration from estuarine waters collected from a mangrove forest in Laem Son National Park, Ranong Province, Thailand, on different occasions. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and the internal transcribed spacer region and phylogenetic analysis, three strains were found to represent two novel Candida species. Two strains (RV5T and RV140) represented a single novel species, for which the name Candida laemsonensis sp. nov. is proposed. The type strain is RV5T (=BCC 35154T =NBRC 105873T =CBS 11419T). Strain R31T was assigned to a novel species that was named Candida andamanensis sp. nov. (type strain R31T =BCC 25965T =NBRC 103862T =CBS 10859T). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and phylogenetic analysis, strains RS17 and RS28T represented another novel species of Candida, for which the name Candida ranongensis sp. nov. is proposed. The type strain is RS28T (=BCC 25964T =NBRC 103861T =CBS 10861T).

Description of Diutina gen. nov., Diutina siamensis, f.a. sp. nov., and reassignment of Candida catenulata, Candida mesorugosa, Candida neorugosa, Candida pseudorugosa, Candida ranongensis, Candida rugosa and Candida scorzettiae to the genus Diutina

“…Members of the proposed genus originate from a diversity of sources (), but an ecological generalization does not seem possible in view of the small number of known specimens. Isolation substrates include a leg wound ( C. neorugosa ), faeces ( C. rugosa and C. catenulata ), sputum ( C. pseudorugosa ), blood ( C. mesorugosa ), rotten oak wood ( C. scorzettiae ), and estuarine water ( C. ranongensis ), as well as soil and spoiled food (Li et al, 2006; Middelhoven & Kurtzman, 2007; Am-In et al , 2011; Lachance et al , 2011; Paredes et al , 2012; Chaves et al , 2013). The present study adds three strains of a novel species isolated from rice leaf tissue and four strains of C. scorzettiae recovered from estuarine water, soil, and fruit flies.…”

“…The species are known from a few isolates from clinical specimens, insects, rotting wood, dung, or spoiled food. Three more relatives not included in the monograph, Candida mesorugosa , Candida neorugosa and Candida pseudorugosa , have been isolated from clinical materials (Li et al , 2006; Paredes et al , 2012; Chaves et al , 2013) and another, Candida ranongensis , was found in a small number of estuarine water samples in Thailand (Am-In et al , 2011). Addition of new taxa did little to provide a more satisfactory placement of the clade from sequences of the D1/D2 domains of the large subunit rRNA gene.…”

Assessment of Diversity of Culturable Marine Yeasts Associated with Corals and Zoanthids in the Gulf of Thailand, South China Sea

“…Thailand is a tropical country, rich in biodiversity in plants, animals, and microorganisms (bacteria, yeasts, and filamentous fungi); however, the microbial diversity, especially that of yeasts, has received less attention compared to the diversity of plants and animals. Only a few reports have been published to date on yeast diversity in natural habitats, for example, on the surface and tissue of plant leaves [22][23][24], the soils and peat soils in peat swamp forests [25,26], and water and sediment in a mangrove forest [27][28][29]. Fungi in marine environments, including yeasts, are far less studied than fungi in terrestrial environments, not only in Thailand but also all over the world.…”

Kluyveromyces aestuarii, a potential environmental quality indicator yeast for mangroves in the State of Rio de Janeiro, Brazil

“…Pichia spartinae from southeastern U. S. salt marshes and associated with the marsh grass Spartina alterniflora, (2,9,20,21), and various new species including some in the Saccharomyces clade have been described recently from mangroves confirming the yeast species richness of this biome (4,5,13,19). Yeasts of the genus Kluyveromyces have been found frequently in these environments.…”