Candida–Bacterial Biofilms and Host–Microbe Interactions in Oral Diseases

Negrini, Thaís de Cássia; Koo, Hyun; Arthur, Rodrigo Alex

doi:10.1007/978-3-030-28524-1_10

Cited by 37 publications

(39 citation statements)

References 150 publications

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“…Interestingly, the interaction between C. albicans and P. brasilensis in dual-species biofilms seem to happen both by direct physical contact and mediated by the extracellular polysaccharide matrix. It is known that some Candida surface adhesins, such as Als3, exert an important role on Candida biofilm formation as well as mediating the co-aggregation between Candida and other microorganisms (Verstrepen and Klis, 2006;Cavalheiro and Teixeira, 2018;Negrini et al, 2019). Moreover, Candida cell-wall mannan-residues might also act as an adhesion element for extracellular polysaccharides (Negrini et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…It is known that some Candida surface adhesins, such as Als3, exert an important role on Candida biofilm formation as well as mediating the co-aggregation between Candida and other microorganisms (Verstrepen and Klis, 2006;Cavalheiro and Teixeira, 2018;Negrini et al, 2019). Moreover, Candida cell-wall mannan-residues might also act as an adhesion element for extracellular polysaccharides (Negrini et al, 2019). In addition, several Paracoccidioides adhesin proteins have been shown to be extracellular matrix ligands, such as 43 kDa glycoprotein (gp43), enolase and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Vicentini et al, 1994;Mendes-Giannini et al, 2004;Barbosa et al, 2006;Donofrio et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Candida albicans is one of the most abundant fungi in the oral mycobiome (Ramage et al, 2004;Dongari-Bagtzoglou et al, 2009;Millsop and Fazel, 2016). Increasing evidence indicate that the coexistence of C. albicans and oral bacteria as well as other interactions with different kingdoms affect both the dynamics of biofilm growth and the course and severity of mucosal lesions, as reviewed by Negrini et al (2019).…”

Section: Introductionmentioning

confidence: 99%

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Dynamics of Mono- and Dual-Species Biofilm Formation and Interactions Between Paracoccidioides brasiliensis and Candida albicans

et al. 2020

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The oral cavity is a highly diverse microbial environment in which microorganisms interact with each other, growing as biofilms on biotic and abiotic surfaces. Understanding the interaction among oral microbiota counterparts is pivotal for clarifying the pathogenesis of oral diseases. Candida spp. is one of the most abundant fungi in the oral mycobiome with the ability to cause severe soft tissue lesions under certain conditions. Paracoccidioides spp., the causative agent of paracoccidioidomycosis, may also colonize the oral cavity leading to soft tissue damage. It was hypothesized that both fungi can interact with each other, increasing the growth of the biofilm and its virulence, which in turn can lead to a more aggressive infectivity. Therefore, this study aimed to evaluate the dynamics of mono-and dual-species biofilm growth of Paracoccidioides brasiliensis and Candida albicans and their infectivity using the Galleria mellonella model. Biomass and fungi metabolic activity were determined by the crystal violet and the tetrazolium salt reduction tests (XTT), respectively, and the colony-forming unit (CFU) was obtained by plating. Biofilm structure was characterized by both scanning electronic-and confocal laser scanning-microscopy techniques. Survival analysis of G. mellonella was evaluated to assess infectivity. Our results showed that dual-species biofilm with P. brasiliensis plus C. albicans presented a higher biomass, higher metabolic activity and CFU than their mono-species biofilms. Furthermore, G. mellonella larvae infected with P. brasiliensis plus C. albicans presented a decrease in the survival rate compared to those infected with P. brasiliensis or C. albicans, mainly in the form of biofilms. Our data indicate that P. brasiliensis and C. albicans coexistence is likely to occur on oral mucosal biofilms, as per in vitro and in vivo analysis. These data further widen the knowledge associated with the dynamics of fungal biofilm growth that can potentially lead to the discovery of new therapeutic strategies for these infections.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dynamics of Mono- and Dual-Species Biofilm Formation and Interactions Between Paracoccidioides brasiliensis and Candida albicans

et al. 2020

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“…e effect of microbial diversity and the interactions in microbial communities cannot be ignored. For example, with respect to the interaction between Candida albicans and oral streptococci, the overuse of broad antibiotics or antibacterial agents occasionally causes the overgrowth of fungi, such as C. albicans, in the oral cavity [127][128][129]. C. albicans is also found in dental carries lesions among children and the elderly and plays an important role in the development of dental carries [130][131][132][133][134].…”

Section: Consideration Of Microbial Interactions In Dental Plaquementioning

confidence: 99%

Application of Antibiotics/Antimicrobial Agents on Dental Caries

Qiu

Zhou

et al. 2020

BioMed Research International

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Dental caries is the most common oral disease. The bacteriological aetiology of dental caries promotes the use of antibiotics or antimicrobial agents to prevent this type of oral infectious disease. Antibiotics have been developed for more than 80 years since Fleming discovered penicillin in 1928, and systemic antibiotics have been used to treat dental caries for a long time. However, new types of antimicrobial agents have been developed to fight against dental caries. The purpose of this review is to focus on the application of systemic antibiotics and other antimicrobial agents with respect to their clinical use to date, including the history of their development, and their side effects, uses, structure types, and molecular mechanisms to promote a better understanding of the importance of microbial interactions in dental plaque and combinational treatments.

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“…Coaggregation of these microorganisms as the primary colonizers of oral biofilm with Candida could enhance its filamentation and increase its pathogenicity. 26,27 Table 1 shows the studies related to infection of oral mucosa models with Candida species alone or in association with other bacteria.…”

Section: Candidamentioning

confidence: 99%

Three-DimensionalIn VitroOral Mucosa Models of Fungal and Bacterial Infections

Tabatabaei

Moharamzadeh

Tayebi

2020

Tissue Engineering Part B: Reviews

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Oral mucosa is the target tissue for many microorganisms involved in periodontitis and other infectious diseases affecting the oral cavity. Three-dimensional (3D) in vitro and ex vivo oral mucosa equivalents have been used for oral disease modeling and investigation of the mechanisms of oral bacterial and fungal infections. This review was conducted to analyze different studies using 3D oral mucosa models for the evaluation of the interactions of different microorganisms with oral mucosa. In this study, based on our inclusion criteria, 43 articles were selected and analyzed. Different types of 3D oral mucosa models of bacterial and fungal infections were discussed in terms of the biological system used, culture conditions, method of infection, and the biological endpoints assessed in each study. The critical analysis revealed some contradictory reports in this field of research in the literature. Challenges in recovering bacteria from oral mucosa models were further discussed, suggesting possible future directions in microbiomics, including the use of oral mucosa-on-a-chip. The potential use of these 3D tissue models for the evaluation of the effects of antiseptic agents on bacteria and oral mucosa was also addressed. This review concluded that there were many aspects that would require optimization and standardization with regard to using oral mucosal models for infection by microorganisms. Using new technologies-such as microfluidics and bioreactors-could help to reproduce some of the physiologically relevant conditions and further simulate the clinical situation.

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Candida–Bacterial Biofilms and Host–Microbe Interactions in Oral Diseases

Cited by 37 publications

References 150 publications

Dynamics of Mono- and Dual-Species Biofilm Formation and Interactions Between Paracoccidioides brasiliensis and Candida albicans

Dynamics of Mono- and Dual-Species Biofilm Formation and Interactions Between Paracoccidioides brasiliensis and Candida albicans

Application of Antibiotics/Antimicrobial Agents on Dental Caries

Three-DimensionalIn VitroOral Mucosa Models of Fungal and Bacterial Infections

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