Abstract:Candida spp. can cause mild-to-severe human infections. Certain species have been described as the etiologic agent of human mastitis, inflammation of the breast tissue. Mastitis affects millions of lactating women and can be a source of disease transmission to the infant. In this work, we report the detection of the unusual etiologic agent of human mastitis, Candida guilliermondii, isolated from the milk of a puerperal woman with subacute mastitis in Rio de Janeiro, Brazil. Species identification was performed… Show more
“…1 C and D), an atypical color for this substrate according to the manufacturer, were observed, indicating the growth of species other than Candida spp. Biochemical culture characteristics were then examined by conventional microscopy methods [ 32 ], and the morphological and phenotypical characteristics of the growths suggested the presence of Trichosporon sp. Fig.…”
Section: Resultsmentioning
confidence: 99%
“…In addition to morphologic and phenotypic tests, the obtained isolates were also identified by MALDI-TOF MS following Pinto et al [ 32 ] and Oliveira et al [ 33 ], employing α-cyano-4-hydroxycinnamic acid (CHCA, Fluka, Buchs, Switzerland) used as the matrix. Each sample was analyzed in triplicate from the same culture and from sub-cultures on alternate days.…”
Yeast infections have gained significant attention in the field of marine biology in recent years. Among the broad diversity of marine organisms affected by these infections, elasmobranchs (sharks and rays) have emerged as highly susceptible, due to climate change effects, such as increasing water temperatures and pollution, which can alter the composition and abundance of fungal communities. Additionally, injuries, or compromised immune systems resulting from pollution or disease may increase the likelihood of fungal infections in elasmobranchs. Studies are, however, still lacking for this taxonomic group. In this context, this study aimed to screen yeast species in cell cultures obtained from the brain of artisanally captured Pseudobatos horkelii, a cartilaginous fish that, although endangered, is highly captured and consumed worldwide. Fungi were isolated during an attempt to establish primary cultures of elasmobranch neural cells. Culture flasks were swabbed and investigated using morphological, phenotypic, and molecular techniques. Two isolates of the emerging opportunistic pathogen Trichosporon japonicum were identified, with high scores (1.80 and 1.85, respectively) by the MALDI-ToF technique. This is the first report of the basidiomycetous yeast T. japonicum in Pseudobatos horkelii in Brazil. This finding highlights the need for further research to determine the potential impact on elasmobranch health, ecology, as well as on commercial fisheries.
“…1 C and D), an atypical color for this substrate according to the manufacturer, were observed, indicating the growth of species other than Candida spp. Biochemical culture characteristics were then examined by conventional microscopy methods [ 32 ], and the morphological and phenotypical characteristics of the growths suggested the presence of Trichosporon sp. Fig.…”
Section: Resultsmentioning
confidence: 99%
“…In addition to morphologic and phenotypic tests, the obtained isolates were also identified by MALDI-TOF MS following Pinto et al [ 32 ] and Oliveira et al [ 33 ], employing α-cyano-4-hydroxycinnamic acid (CHCA, Fluka, Buchs, Switzerland) used as the matrix. Each sample was analyzed in triplicate from the same culture and from sub-cultures on alternate days.…”
Yeast infections have gained significant attention in the field of marine biology in recent years. Among the broad diversity of marine organisms affected by these infections, elasmobranchs (sharks and rays) have emerged as highly susceptible, due to climate change effects, such as increasing water temperatures and pollution, which can alter the composition and abundance of fungal communities. Additionally, injuries, or compromised immune systems resulting from pollution or disease may increase the likelihood of fungal infections in elasmobranchs. Studies are, however, still lacking for this taxonomic group. In this context, this study aimed to screen yeast species in cell cultures obtained from the brain of artisanally captured Pseudobatos horkelii, a cartilaginous fish that, although endangered, is highly captured and consumed worldwide. Fungi were isolated during an attempt to establish primary cultures of elasmobranch neural cells. Culture flasks were swabbed and investigated using morphological, phenotypic, and molecular techniques. Two isolates of the emerging opportunistic pathogen Trichosporon japonicum were identified, with high scores (1.80 and 1.85, respectively) by the MALDI-ToF technique. This is the first report of the basidiomycetous yeast T. japonicum in Pseudobatos horkelii in Brazil. This finding highlights the need for further research to determine the potential impact on elasmobranch health, ecology, as well as on commercial fisheries.
“…MALDI-TOF MS identification: isolates were subjected to MALDI-TOF MS, following previously described instructions [18]. Briefly, yeast cells were treated with 70% formic acid and acetonitrile.…”
Human activity directly or indirectly causes climate change, promoting changes in the composition of the atmosphere. This change is beyond the variation of the natural climate. In this manner, climate change could create an environmental pressure which is enough to trigger new fungal diseases. In addition to climate alterations, the onset of the COVID-19 pandemic has also been associated with the emergence of fungal pathogens. Fungi showed that an inability to grow at high temperatures limits the capacity of fungi to infect mammals. However, fungi can develop thermotolerance, gradually adapting to rising temperatures due to climate change, and generating a greater number of disease-causing organisms. In the present study, we reported the detection and identification of Candida palmioleophila isolates recovered from raw sewage samples in Niteroi city, Rio de Janeiro State, Brazil, during a monitoring program for measuring SARS-CoV-2 presence and concentration. Using polyphasic taxonomy to identify the species and evaluating some virulence aspects of this species, such as biofilm formation and extracellular enzyme production, our data highlight this species as a possible emerging pathogen in Brazil, especially in the pandemic context.
“…It is often more resistant to antifungal agents than other Candida strains [2] . Aside from candidemia, Candida guilliermondi has also been linked to osteomyelitis, septic arthritis, endocarditis, mastitis, and skin lesions [3][4][5][6] . Candida cellulitis is a rare disease, only a few cases have been reported.…”
Section: Introductionmentioning
confidence: 99%
“…Candida guilliermondi can cause osteomyelitis, septic arthritis, endocarditis, mastitis, and skin lesions [3][4][5][6] . Facial cellulitis caused by Candida spp.…”
Background: Candida cellulitis is a rare disease, primarily reported in immunocompromised patients. Atypical Candida spp. infections are increasing, largely due to the growing number of immunocompromised patients. This case report describes a 52-year-old immunocompetent patient with facial cellulitis caused by Candida guilliermondi. Candida guilliermondi has not previously been reported as a cause of facial cellulitis in either immunocompromised or immunocompetent patients. Case presentation: A 52-year-old, otherwise healthy, male patient presented with facial cellulitis that did not respond to intravenous antibiotics. Culture of the drained pus revealed Candida guilliermondi. The patient was successfully treated with intravenous fluconazole. Conclusion: This case highlights the possibility of atypical Candida spp. causing deep facial infections in immunocompetent patients. Candida guilliermondi has not been previously reported as a cause of facial cellulitis in either immunocompromised or immunocompetent patients. Healthcare providers should consider atypical Candida spp. infections in the differential diagnosis of deep facial infections in both immunocompromised and immunocompetent patients.
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