Candidate peptide vaccine induced protection against classical swine fever virus

Dong, Xiaonan; Wei, Ke Qiang; Liu, Zu Qiang; Chen, Ying Hua

doi:10.1016/s0264-410x(02)00466-8

Cited by 45 publications

(17 citation statements)

References 27 publications

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“…The glycoprotein E2 is the most immunogenic protein of the classical swine fever virus [6,13]. It has been previously described that vaccination with the E2 glycoprotein plasmid DNA alone and in a primeboost protocol with a recombinant porcine E2 adenovirus could protect pigs from lethal CSFV infection [18].…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination

et al. 2005

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-The aim of this study was to determine the immunomodulatory effects of IL-12, IL-18 and CD154 (CD40 ligand, CD40L) in DNA-vaccination against the classical swine fever virus. Four recombinant plasmids were constructed including the CSFV coding region for the glycoprotein gp55/E2 alone or together with porcine IL-12, IL-18 or CD154 genes. Five groups of four pigs each were immunized intramuscularly (i.m.) three times with the respective constructs. The control group was inoculated with empty plasmid DNA. Eighteen days after the final immunization, the pigs were challenged with a lethal dose of CSFV strain Eystrup and monitored for a further 16 days. This study showed that co-delivery of IL-18 and CD154 induced an earlier appearance of serum antibodies, reduced B-cell deficiency after infection and protected pigs against a lethal CSFV infection. In contrast, co-delivery of IL-12 led to a reduced titer of neutralizing antibodies and protection against a lethal CSFV challenge in comparison to the other pigs and to pigs that were immunized with a gp55/E2 plasmid alone.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination

et al. 2005

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“…their antibody patterns are very similar to those observed in naturally infected animals, making it extremely difficult to differentiate vaccinated animals from infected ones. This has prompted the search for new DIVA vaccine strategies against CSFV, including DNA15, 23–29, viral vector20, 22, 30–33, chimeric pestivirus34–36, and peptide14, 37–42 vaccines. Because peptide vaccines contain no genetic component, no risk of pathogen replication by any vaccine component exists, thus making them practically, by definition, DIVA vaccine candidates.…”

Section: Introductionmentioning

confidence: 99%

“…In the search for peptide‐based CSFV vaccines, several immunogenic peptides corresponding to different regions of the A or B‐C domains of E2 have been proposed and used in either mono‐ or multi‐peptide formulations38–42 which have been evaluated in immunization and challenge experiments. Although some peptide mixtures have been reported to induce CSFV‐specific neutralizing antibodies as well as protective activity against CSFV challenge infection, in most cases they have failed to provide complete protection from clinical signs, viraemia, and virus shedding38, 39.…”

Section: Introductionmentioning

confidence: 99%

Peptide vaccine candidates against classical swine fever virus: T cell and neutralizing antibody responses of dendrimers displaying E2 and NS2–3 epitopes

Monsò

Tarradas

Torre

et al. 2010

Journal of Peptide Science

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Three peptide-based systems integrating B and T antigenic sites of CSFV and displaying the B epitopes in fourfold presentation have been designed and produced, and shown to bring about significant enhancements in immunogenicity over the peptides in monomeric form. Of the different strategies tested for producing the dendrimeric constructs, stepwise SPPS using 3,6-dioxaoctanoic acid as flexible, PEG-like spacer units at the branching points is clearly advantageous, in particular over ligation in solution. The constructs have been used for immunization of domestic pigs, in order to evaluate the protective response induced by each peptide constructs, and to characterize the B- and T-cell response against CSFV in the natural host.

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“…Previous studies shown that the N terminus of CSFV E2 has four antigenic domains (A-D), with three subdomains (A1-A3) in domain A. Subdomain A1 and domain B and C are major neutralizing determinants [15,16]. Based on previous study, the neutralizing epitopes corresponding to different regions of the A or BC domains of E2 were proposed and used as vaccines against CSFV in either mono-or multi-peptide formulations [17-20]. Some mixtures of linear peptides have been reported to induce CSFV-specific neutralizing antibodies as well as protection against CSFV challenge infection.…”

Section: Introductionmentioning

confidence: 99%

Multiple linear B-cell epitopes of classical swine fever virus glycoprotein E2 expressed in E.coli as multiple epitope vaccine induces a protective immune response

Zhou

Liu

Jiang³

et al. 2011

Virol J

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Classical swine fever is a highly contagious disease of swine caused by classical swine fever virus, an OIE list A pathogen. Epitope-based vaccines is one of the current focuses in the development of new vaccines against classical swine fever virus (CSFV). Two B-cell linear epitopes rE2-ba from the E2 glycoprotein of CSFV, rE2-a (CFRREKPFPHRMDCVTTTVENED, aa844-865) and rE2-b (CKEDYRYAISSTNEIGLLGAGGLT, aa693-716), were constructed and heterologously expressed in Escherichia coli as multiple epitope vaccine. Fifteen 6-week-old specified-pathogen-free (SPF) piglets were intramuscularly immunized with epitopes twice at 2-week intervals. All epitope-vaccinated pigs could mount an anamnestic response after booster vaccination with neutralizing antibody titers ranging from 1:16 to 1:256. At this time, the pigs were subjected to challenge infection with a dose of 1 × 106 TCID50 virulent CSFV strain. After challenge infection, all of the rE2-ba-immunized pigs were alive and without symptoms or signs of CSF. In contrast, the control pigs continuously exhibited signs of CSF and had to be euthanized because of severe clinical symptoms at 5 days post challenge infection. The data from in vivo experiments shown that the multiple epitope rE2-ba shown a greater protection (similar to that of HCLV vaccine) than that of mono-epitope peptide(rE2-a or rE2-b). Therefore, The results demonstrated that this multiple epitope peptide expressed in a prokaryotic system can be used as a potential DIVA (differentiating infected from vaccinated animals) vaccine. The E.coli-expressed E2 multiple B-cell linear epitopes retains correct immunogenicity and is able to induce a protective immune response against CSFV infection.

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Candidate peptide vaccine induced protection against classical swine fever virus

Cited by 45 publications

References 27 publications

Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination

Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination

Peptide vaccine candidates against classical swine fever virus: T cell and neutralizing antibody responses of dendrimers displaying E2 and NS2–3 epitopes

Multiple linear B-cell epitopes of classical swine fever virus glycoprotein E2 expressed in E.coli as multiple epitope vaccine induces a protective immune response

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