Candidate Resistance Genes From an Est Database Prove a Rich Source of Markers for Major Genes Conferring Resistance to Important Apple Pests and Diseases

Gardiner, Susan E.; Murdoch, JN; Meech, Sarah B; Rusholme, Rachel L.; Bassett, H.; Cook, Michael B.; Bus, Vincent G. M.; Rikkerink, Erik H. A.; Gleave, Andrew P.; Crowhurst, Ross N.; Ross, G. J.; Warrington, I.A.

doi:10.17660/actahortic.2003.622.12

Cited by 28 publications

(25 citation statements)

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“…Markers developed by Plant and Food Research are those beginning with NZms for the SSR markers, and NZsc for the SCAR markers (Table 1), some of which have been reported previously: NZmsCN943818 (LG3), NZmsDR033893 (LG11) and NZmsPal8 (Celton et al 2009), and NZscAC20, NZscAY17/AB16 and NZscN18 (Gardiner et al 2003). Other published SSR markers used were CH02d12 (LG11) and CH04e05 (LG7) (Liebhard et al 2002) (Yamamoto et al 2002); EMPc111a (LG7) (Fernández-Fernández et al 2006); Hi02c06 (LG11), Hi05b09 (LG7), and Hi07d12b (LG7) (Silfverberg-Dilworth et al 2006); and CH-Sd1 (Khan et al 2007).…”

Section: Dna Isolation Amplification and Genetic Marker Analysismentioning

confidence: 84%

“…As there are no later reports of the Pl-m gene having been overcome, the gene still has value for resistance breeding, provided it is pyramided with other mildew resistance genes to achieve durable resistance. While it was demonstrated previously that the Pl-m and Pl2 genes are linked (Gardiner et al 2003) and might be allelic, they however are different genes or alleles as hosts carrying the Pl-m gene are resistant to a P. leucotricha race overcoming the Pl2 gene in New Zealand (Wood and Bus, unpublished data). The gene maps to the same region of LG11 where a scab QTL identified in 'Fiesta' has been repeatedly mapped (Durel et al 2003(Durel et al , 2004Liebhard et al 2003) as well as one from 'Gala' (Soufflet-Freslon et al 2008).…”

Section: Powdery Mildew Resistance Gene Pl-mmentioning

confidence: 99%

“…However, it did not segregate in the M.9×R5 reference population. A set of SCARs previously found to be linked to other powdery mildew resistance genes was tested, and five SCARS linked to Pl2 were also linked to Pl-m (Gardiner et al 2003). As Pl2 had been assigned to LG11 (Liebhard et al 2002) with the aid of a marker for a major mildew resistance quantitative trait locus in A679-2 (Seglias and Gessler 1997), SSRs from this group were screened in the MIS population to confirm the linkage.…”

Section: Powdery Mildew Resistance Gene Pl-mmentioning

confidence: 99%

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Genome mapping of an apple scab, a powdery mildew and a woolly apple aphid resistance gene from open-pollinated Mildew Immune Selection

Bus

Bassett

Bowatte

et al. 2010

Tree Genetics & Genomes

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Apple is host to a wide range of pests and diseases, with several of these, such as apple scab, powdery mildew and woolly apple aphid, being major causes of damage in most areas around the world. Resistance breeding is an effective way of controlling pests and diseases, provided that the resistance is durable. As the gene pyramiding strategy for increasing durability requires a sufficient supply of resistance genes with different modes of action, the identification and mapping of new resistance genes is an ongoing process in breeding. In this paper, we describe the mapping of an apple scab, a powdery mildew and a woolly apple aphid gene from progeny of openpollinated mildew immune selection. The scab resistance gene Rvi16 was identified in progeny 93.051 G07-098 and mapped to linkage group 3 of apple. The mildew and woolly aphid genes were identified in accession 93.051 G02-054. The woolly aphid resistance gene Er4 mapped to linkage group 7 to a region close to where previously the genes Sd1 and Sd2, for resistance to the rosy apple leaf-curling aphid, had been mapped. The mildew resistance gene Pl-m mapped to the same region on linkage group 11 where Pl2 had been mapped previously. Flanking markers useful for markerassisted selection have been identified for each gene.

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Section: Dna Isolation Amplification and Genetic Marker Analysismentioning

confidence: 84%

Section: Powdery Mildew Resistance Gene Pl-mmentioning

confidence: 99%

Section: Powdery Mildew Resistance Gene Pl-mmentioning

confidence: 99%

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Genome mapping of an apple scab, a powdery mildew and a woolly apple aphid resistance gene from open-pollinated Mildew Immune Selection

Bus

Bassett

Bowatte

et al. 2010

Tree Genetics & Genomes

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“…Previously, Gardiner et al (2003) established that ESTs often clustered around R genes of different classes within a region of several cM in the apple genome. In this study, RGA and E-STS loci were mapped on all linkage groups, except for LG3 and LG6.…”

Section: Mapping Of E-sts Markersmentioning

confidence: 99%

Development and linkage mapping of E-STS and RGA markers for functional gene homologues in apple

Naik

Hampson

Gašić

et al. 2006

Genome

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Linkage maps developed from known-function genes can be valuable in the candidate gene mapping approach. A set of 121 expressed sequence tagged site (E-STS) primer pairs were tested on a framework genetic linkage map of apple (Malus x domestica Borkh.) constructed using simple sequence repeats (SSRs) and randomly amplified polymorphic DNA (RAPD) markers. These known-function gene markers, E-STSs, were supplemented by markers for resistance gene analogues (RGAs), designed based on conserved motifs in all characterized resistance genes isolated from plant species. A total of 229 markers, including 46 apple E-STSs, 8 RGAs, 85 SSRs from apple and peach, and 88 RAPDs, were assigned to 17 linkage groups covering 832 cM of the apple genome, based on 52 individuals originating from the cross 'Antonovka debnicka' (Q12-4) x 'Summerred'. Clusters of E-STS and RGA loci were located in linkage groups previously identified to carry resistance genes, some of which confer resistance to apple scab disease caused by Venturia inaequalis (Cke.) Wint.

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“…1999; Gardiner et al 2003;Quamme et al 2003;Tartarini et al 2004;Calenge et al 2005;Naik et al 2006). Molecular markers are now available for pyramiding several major resistance genes (Bus et al 2005a, b;Boudichevskaia et al 2006;Gessler et al 2006).…”

mentioning

confidence: 99%

A study of scab resistance in 16 apple progenies using parents with partial scab resistance

Hampson¹,

Quamme²,

Sholberg³

2009

Can. J. Plant Sci.

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. 2009. A study of scab resistance in 16 apple progenies using parents with partial scab resistance. Can. J. Plant Sci. 89: 693Á699. A study was conducted to examine the inheritance of partial resistance to apple scab [Venturia inaequalis (Cke.) Wint.] using as parents a set of apple (Malus )domestica Borkh.) cultivars that show a range of field resistance to the disease, but are not known to carry any major-gene resistance. Akane, Alkmene, Antonovka, Carola, Chehalis, Goldgelbe, Katja and Summerred were used as pollen parents, and Spartan and an unnamed selection of Splendour )Gala parentage were used as seed parents in a factorial 2 )8 mating design. One hundred seedlings of each family were planted in a randomized complete block design at a site with heavy annual natural scab infections. Each seedling was rated for infection severity for 3 consecutive years using a six-point categorical scale. A x 2 procedure was used to estimate the contribution of each parent to field resistance (analogous to general combining ability, GCA) and the contribution of the particular male-female combination (analogous to specific combining ability, SCA). Infection severity tended to increase over the 3 yr of the study. Antonovka and Chehalis had the highest analogous GCA for resistance (defined here as absence of sporulating lesions). Specific combining ability was present in 2 of 3 yr, but was small in magnitude relative to GCA. The field resistance of a given parent tended to be a poor predictor of its breeding value. However, 13 of the 16 families had at least 30% of seedlings with a potentially useful degree of partial resistance (low severity of infection). The results are discussed in the context of breeding apples with durable scab resistance. . Ils ont utilise´pour cela les parents d'un groupe de cultivars de pommier (Malus )domestica Borkh.) affichant une re´sistance variable a`la maladie sur le terrain, mais qu'on ignorait porter ou pas d'importants ge`nes de re´sistance. Les cultivars Akane, Alkmene, Antonovka, Carola, Chehalis, Goldgelbe, Katja et Summerred ont servi a`produire le pollen qui a fertilise´la varie´teŚ partan et une se´lection sans nom d'ascendance Splendour )Gala pour la production de graines, dans le cadre d'un essai de reproduction factoriel 2 )8. Cent plantules de chaque famille ont ensuite e´te´repique´s au hasard en blocs ale´atoires complets, a`un endroit marque´par d'importantes infections naturelles de tavelure chaque anne´e. Chaque plantule a e´teń ote´e selon la gravite´de l'infection pendant trois anne´es conse´cutives, au moyen d'une e´chelle a`6 points. Une technique x 2 a permis d'estimer l'apport de chaque parent a`la re´sistance sur le terrain (valeur analogue a`l'aptitude ge´ne´rale a`la combinaison, GCA) et la contribution du couple maˆle-femelle particulier (valeur analogue a`l'aptitude spe´cifique a`la combinaison, SCA). Le degre´d'infection a eu tendance a`augmenter au cours des trois anne´es qu'a dure´es l'e´tude. Les cultivars Antonovka et Chehalis pre´sentaient la plus forte val...

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Candidate Resistance Genes From an Est Database Prove a Rich Source of Markers for Major Genes Conferring Resistance to Important Apple Pests and Diseases

Cited by 28 publications

References 0 publications

Genome mapping of an apple scab, a powdery mildew and a woolly apple aphid resistance gene from open-pollinated Mildew Immune Selection

Genome mapping of an apple scab, a powdery mildew and a woolly apple aphid resistance gene from open-pollinated Mildew Immune Selection

Development and linkage mapping of E-STS and RGA markers for functional gene homologues in apple

A study of scab resistance in 16 apple progenies using parents with partial scab resistance

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