Candidin and Trichophytin Stimulate the Production of Th1 and Regulatory Cytokines by Peripheral Blood Mononuclear Cells: Implication for Their Use as Adjuvants in Immunotherapy

Amor, Ana Lúcia Moreno; Santos, Leonardo Nascimento; Galvão, Alana Alcântara; Belitardo, Emília Maria Medeiros de Andrade; Silva, Eduardo Santos; Alcântara-Neves, Neuza Maria; Pontes-de-Carvalho, Lain Carlos

doi:10.2217/imt.14.89

Cited by 4 publications

(3 citation statements)

References 38 publications

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“…Peripheral blood from allergic ( N = 6) and non‐allergic ( N = 6) patients was used to obtain PBMC 23 . Cells were stimulated for 120 h with 20 μg/ml of DpE, 12.5 μg/ml of rDer p 2231; 10 μg/ml of pokeweed mitogen (PWM) was used as a positive control 23–27 . Supernatants were used to quantify a total of 18 cytokines, which were detected simultaneously using a multiplex human cytokine/chemokine magnetic bead panel ( MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel ‐ Immunology Multiplex Assay, Millipore), following the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…23 Cells were stimulated for 120 h with 20 μg/ml of DpE, 12.5 μg/ml of rDer p 2231; 10 μg/ml of pokeweed mitogen (PWM) was used as a positive control. [23][24][25][26][27] Supernatants were used to quantify a total of 18 cytokines, which were detected simultaneously using a multiplex human cytokine/ chemokine magnetic bead panel (MILLIPLEX MAP Human Cytokine/ Chemokine Magnetic Bead Panel -Immunology Multiplex Assay, Millipore), following the manufacturer's instructions. Mean fluorescent intensities were determined on a MAGPIX® System and serum concentration was calculated according to the standard curve and analyzed using MILLIPLEX® Analyst 5.1 (Merck KGaA, Darmstadt, Germany).…”

Section: Culture Of Peripheral Blood Mononuclear Cells For the Determ...mentioning

confidence: 99%

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Recombinant T‐cell epitope conjugation: A new approach for Dermatophagoides hypoallergen design

Fernandes

Silva

Silveira

et al. 2022

Clin Experimental Allergy

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Background: Allergen-specific immunotherapy (AIT) is the only clinical approach that can potentially cure some allergic diseases by inducing immunological tolerance.Dermatophagoides pteronyssinus is considered as the most important source of mite allergens worldwide, with high sensitization rates for the major allergens Der p 1, Der p 2 and Der p 23. The aim of this work is to generate a hypoallergenic hybrid molecule containing T-cell epitopes from these three major allergens. Methods: The hybrid protein termed Der p 2231 containing T-cell epitopes was purified by affinity chromatography. The human IgE reactivity was verified by comparing those with the parental allergens. The hybrid was also characterized immunologically through an in vivo mice model. Results: The hybrid rDer p 2231 stimulated in peripheral blood mononuclear cells (PBMCs) isolated from allergic patients with higher levels of IL-2, IL-10, IL-15 and IFNγ, as well as lower levels of IL-4, IL-5, IL-13, TNFα and GM-CSF. The use of hybrid molecules as a therapeutic model in D. pteronyssinus allergic mice led to the reduction of IgE production and lower eosinophilic peroxidase activity in the airways. We found increased levels of IgG antibodies that blocked the IgE binding to the parental allergens in the serum of allergic patients. Furthermore, the stimulation of splenocytes from mice treated with rDer p 2231 induced higher levels of IL-10 and IFNγ and decreased the secretion of IL-4 and IL-5, when compared with parental allergens and D. pteronyssinus extract. Conclusions: rDer p 2231 has the potential to be used in AIT in patients co-sensitized with D. pteronyssinus major allergens, once it was able to reduce IgE production, inducing allergen-specific blocking antibodies, restoring and balancing Th1/Th2 immune responses, and inducing regulatory T-cells.

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Section: Methodsmentioning

confidence: 99%

Section: Culture Of Peripheral Blood Mononuclear Cells For the Determ...mentioning

confidence: 99%

Recombinant T‐cell epitope conjugation: A new approach for Dermatophagoides hypoallergen design

Fernandes

Silva

Silveira

et al. 2022

Clin Experimental Allergy

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show abstract

“…Adjuvants can promote the reaction of T cells or B cells by enhancing the activity of macrophages, and participate in hapten or antigen immune response [ 7 ]. With the research on the vaccine and host immune response caused by N. caninum , cytokines as new molecular adjuvants can enhance the specific immune response of vaccines and have been widely studied [ 8 ]. Cytokines are a type of cell regulatory protein synthesized and secreted by immune cells and nonimmune cells after stimulation and can induce the differentiation and maturation of antigen-presenting cells, improve their antigen-presenting ability, help immune cells identify pathogens and eliminate pathogens [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Protective Immune Responses Induced in BALB/c Mice and Goats by the Neospora caninum Surface SRS Proteins and Interleukin-18

Wang

et al. 2022

Animals

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Neosporosis is caused by Neospora caninum (N. caninum), which mainly infects cattle and goats and severely threatens the animal industry. In this study, the inhibitory effects of polyclonal antiserum anti-NcSRS17, NcSRS2 and NcSRS52 were explored. Cytokines in mice or goat serum were detected after immunization. After infection, the survival of mice was recorded. The pathological changes and parasite loads were observed and detected in tissues. The results showed that anti-NcSRS2, NcSRS17 and NcSRS52 antibodies all inhibit the invasion and proliferation of N. caninum. The IFN-γ level in the NcSRS17 group was higher than that in the NcSRS2 and NcSRS52 groups, and higher in the NcSRS2-mIL-18 group than in the NcSRS2 group. The survival rates of mice were 16% in the positive control group, 67% in the SRS52 group, 83% in the SRS2 and mIL-18 groups and 100% in the SRS17 and SRS2-mIL-18 groups. Goats immunized with NcSRS17-gIL-18 developed high levels of IL-4, IL-12 and IFN-γ compared with those immunized with NcSRS-17. Parasite loads in the brains of animals in the NcSRS17 and NcSRS17-gIL-18 groups were significantly reduced, and were significantly lower in the NcSRS17-gIL-18 group (p ≤ 0.01). This study indicates that SRS17 may be an antigen candidate for vaccine development against neosporosis, and IL-18 can enhance the immune protective efficiency of antigen candidates.

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Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro

et al. 2022

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Candidin and Trichophytin Stimulate the Production of Th1 and Regulatory Cytokines by Peripheral Blood Mononuclear Cells: Implication for Their Use as Adjuvants in Immunotherapy

Cited by 4 publications

References 38 publications

Recombinant T‐cell epitope conjugation: A new approach for Dermatophagoides hypoallergen design

Recombinant T‐cell epitope conjugation: A new approach for Dermatophagoides hypoallergen design

Evaluation of Protective Immune Responses Induced in BALB/c Mice and Goats by the Neospora caninum Surface SRS Proteins and Interleukin-18

Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro

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