Cangxitongbi capsules protect the articular cartilage in the rat knee through the long non-coding RNA HOTAIR/p38MAPK pathway

Song, Xu-Yu; Zhao, Min; Zhang, Peng; Yang, Lingsen; Bi, Rong‐Xiu; Xie, Wen‐Peng

doi:10.21037/atm-21-6539

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…As a widely used herbal medicine for dispelling wind, removing dampness, and relieving pain, APR has been applied in East Asian countries for thousands of years [ 9 ]. Our previous studies have shown that the Cangxitongbi (CXTB) capsule, a Chinese patent medicine mainly composed of APR, can protect articular cartilage by enhancing autophagy, suppressing inflammation, and inhibiting cartilage extra-cellular matrix (ECM) degradation [ 10 , 11 ]. Given the high content of osthole in CXTB, it is necessary to further verify the mechanism of osthole in inhibiting KOA cartilage degeneration.…”

Section: Introductionmentioning

confidence: 99%

“…Autophagy is considered to be essential for maintaining homeostasis in eukaryotic cells, and chondrocytes in the early stage of OA are protected by autophagy [ 15 , 16 ]. Given the role of CXTB capsule as well as osthole in the treatment of KOA, we hypothesized that osthole may activate autophagy to exert its anti-KOA effect by targeting the AMPK/ULK1 pathway [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Osthole Suppresses Knee Osteoarthritis Development by Enhancing Autophagy Activated via the AMPK/ULK1 Pathway

Wang

et al. 2022

Molecules

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Knee osteoarthritis (KOA) is an increasingly prevalent heterogeneous disease characterized by cartilage erosion and inflammation. As the main chemical constituent of Angelicae Pubescentis Radix (APR), an anti-inflammatory herbal medicine, the potential biological effects and underlying mechanism of osthole on chondrocytes and KOA progression remain elusive. In this study, the potential effect and mechanism of osthole on KOA were investigated in vitro and in vivo. We found that osthole inhibited IL-1β-induced apoptosis and cartilage matrix degeneration by activating autophagy in rat chondrocytes. In addition, osthole could activate autophagy through phosphorylation of AMPK/ULK1, and AMPK serves as a positive upstream regulator of ULK1. Furthermore, KOA rats treated with osthole showed phosphorylation of the AMPK/ULK1 pathway and autophagy activation, as well as cartilage protection. Collectively, the AMPK/ULK1 signaling pathway can be activated by osthole to enhance autophagy, thereby suppressing KOA development. Osthole may be a novel and effective therapeutic agent for the clinical treatment of KOA.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Osthole Suppresses Knee Osteoarthritis Development by Enhancing Autophagy Activated via the AMPK/ULK1 Pathway

Wang

et al. 2022

Molecules

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“…Forty, eight-week-old adult female Wistar rats (SLAC, Shanghai, China), SPF grade, weighing 220–240 g, were fed adaptively for 1 week, and an OA model was constructed using the modified Hulth method ( 17 ). The model group, consisting of 20 rats, was anesthetized by a peritoneal injection of 2% pentobarbital sodium solution (Sinopharm, Beijing, China) and fixed in the lateral position.…”

Section: Methodsmentioning

confidence: 99%

Exosomes derived from miR-146a-overexpressing fibroblast-like synoviocytes in cartilage degradation and macrophage M1 polarization: a novel protective agent for osteoarthritis?

Wang,

Zhang,

Zhang

et al. 2024

Front. Immunol.

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IntroductionPathological changes in the articular cartilage (AC) and synovium are major manifestations of osteoarthritis (OA) and are strongly associated with pain and functional limitations. Exosome-derived microRNAs (miRNAs) are crucial regulatory factors in intercellular communication and can influence the progression of OA by participating in the degradation of chondrocytes and the phenotypic transformation in the polarization of synovial macrophages. However, the specific relationships and pathways of action of exosomal miRNAs in the pathological progression of OA in both cartilage and synovium remain unclear.MethodsThis study evaluates the effects of fibroblast-like synoviocyte (FLS)-derived exosomes (FLS-Exos), influenced by miR-146a, on AC degradation and synovial macrophage polarization. We investigated the targeted relationship between miR-146a and TRAF6, both in vivo and in vitro, along with the involvement of the NF-κB signaling pathway.ResultsThe expression of miR-146a in the synovial exosomes of OA rats was significantly higher than in healthy rats. In vitro, the upregulation of miR-146a reduced chondrocyte apoptosis, whereas its downregulation had the opposite effect. In vivo, exosomes derived from miR-146a-overexpressing FLSs (miR-146a-FLS-Exos) reduced AC injury and chondrocyte apoptosis in OA. Furthermore, synovial proliferation was reduced, and the polarization of synovial macrophages shifted from M1 to M2. Mechanistically, the expression of TRAF6 was inhibited by targeting miR-146a, thereby modulating the Toll-like receptor 4/TRAF6/NF-κB pathway in the innate immune response.DiscussionThese findings suggest that miR-146a, mediated through FLS-Exos, may alleviate OA progression by modulating cartilage degradation and macrophage polarization, implicating the NF-κB pathway in the innate immune response. These insights highlight the therapeutic potential of miR-146a as a protective agent in OA, underscoring the importance of exosomal miRNAs in the pathogenesis and potential treatment of the disease.

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“…The rats were anesthetized with iso urane (1.5-2.0%) and disinfected with skin application. Brie y, 30 rats underwent a patellar incision on the medical side of the knee [20]. The medial collateral ligament and anterior cruciate ligament were severed, and the medial meniscus used to be resected.…”

Section: Koa Rat Modelmentioning

confidence: 99%

Verification of the therapeutic effects and neural mechanism of Bushen Zhuangjin Decoction in the treatment of knee osteoarthritis

Tan

Zheng

Lin

et al. 2023

Preprint

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Chronic pain is the principal clinical manifestation of knee osteoarthritis (KOA) and an essential indicator of the diagnosis and treatment effect. Changes in brain functional activity are related with chronic pain in KOA. Bushen Zhuangjin Decoction (BZD) has been proved to reduce inflammation of arthritis, improve cartilage degeneration and analgesia, but whether it plays a role through the change of brain function activity is not clear. Here, three experiments were performed: (1) network pharmacology evaluation to discover the potential targets of BZD to relieve pain in KOA; (2) verification of the therapeutic effects of BZD treatment on KOA pain with histomorphology, behavioral assessments, suspension chip analysis, and ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) assays; and (3) functional magnetic resonance imaging to explore the effects of BZD treatment on brain function associated to KOA. The analgesic effect of BZD on KOA was found to be related to the neurotransmitters of pain signals through network pharmacology and the therapeutic effect of BZD on KOA pain was verified in vivo, and related to neuropeptides and neurotransmitters. Functional magnetic resonance imaging showed that BZD treatment could reverse the regional homogeneity/amplitude of low-frequency fluctuation analysis in pain-related brain regions of KOA, suggesting that the analgesic mechanism of BZD is related to neural regulation. This study confirmed the key position of pain-related neuromodulation mechanisms in the analgesic therapy of BZD and provide a theoretical basis for the treatment of KOA pain with BZD as a traditional Chinese medical.

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Cangxitongbi capsules protect the articular cartilage in the rat knee through the long non-coding RNA HOTAIR/p38MAPK pathway

Cited by 7 publications

References 23 publications

Osthole Suppresses Knee Osteoarthritis Development by Enhancing Autophagy Activated via the AMPK/ULK1 Pathway

Osthole Suppresses Knee Osteoarthritis Development by Enhancing Autophagy Activated via the AMPK/ULK1 Pathway

Exosomes derived from miR-146a-overexpressing fibroblast-like synoviocytes in cartilage degradation and macrophage M1 polarization: a novel protective agent for osteoarthritis?

Verification of the therapeutic effects and neural mechanism of Bushen Zhuangjin Decoction in the treatment of knee osteoarthritis

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