Canine adipose tissue-derived mesenchymal stem cells ameliorate severe acute pancreatitis by regulating T cells in rats

Kim, Hyun‐Wook; Song, Woo‐Jin; Li, Qiang; Han, Sei-Myoung; Jeon, Ki‐Hyun; Park, Sang-Chul; Ryu, Min-Ok; Chae, Hyung‐Kyu; Kweon, Kyeong; Youn, Hwa‐Young

doi:10.4142/jvs.2016.17.4.539

Cited by 30 publications

(24 citation statements)

References 38 publications

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“…As shown in acute pancreatitis models, 16,29,[39][40][41] we also found that the protective effects of MSC therapy were multifactorial and targeted several cell types in the pancreas. First, ASCs protected pancreatic cells from ethanol-and cerulean-induced cell death.…”

Section: Discussionsupporting

confidence: 66%

“…14 Compared with MSCs from other tissues, ASCs display greater regenerative capacity based on their differentiation and paracrine activities. 15 The protective effects of ASCs have been shown in rodent models of severe acute pancreatitis, 16 type 1 diabetes, 17,18 experimental autoimmune encephalomyelitis, 19 rheumatoid arthritis, systemic lupus erythematosus, and other disorders. 20,21 ASCs are currently being tested in clinical trials for the treatment of Crohn'srelated rectovaginal fistula, 22 refractory rheumatoid arthritis, 23 severe osteoarthritis of the knee, 24 systemic sclerosis, 25 and other diseases.…”

Section: Introductionmentioning

confidence: 99%

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Adipose Stem Cell Therapy Mitigates Chronic Pancreatitis via Differentiation into Acinar-like Cells in Mice

et al. 2017

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The objective of this study was to assess the capacity of adipose-derived mesenchymal stem cells (ASCs) to mitigate disease progression in an experimental chronic pancreatitis mouse model. Chronic pancreatitis (CP) was induced in C57BL/6 mice by repeated ethanol and cerulein injection, and mice were then infused with 4 × 10 or 1 × 10 GFP ASCs. Pancreas morphology, fibrosis, inflammation, and presence of GFP ASCs in pancreases were assessed 2 weeks after treatment. We found that ASC infusion attenuated pancreatic damage, preserved pancreas morphology, and reduced pancreatic fibrosis and cell death. GFP ASCs migrated to pancreas and differentiated into amylase cells. In further confirmation of the plasticity of ASCs, ASCs co-cultured with acinar cells in a Transwell system differentiated into amylase cells with increased expression of acinar cell-specific genes including amylase and chymoB1. Furthermore, culture of acinar or pancreatic stellate cell lines in ASC-conditioned medium attenuated ethanol and cerulein-induced pro-inflammatory cytokine production in vitro. Our data show that a single intravenous injection of ASCs ameliorated CP progression, likely by directly differentiating into acinar-like cells and by suppressing inflammation, fibrosis, and pancreatic tissue damage. These results suggest that ASC cell therapy has the potential to be a valuable treatment for patients with pancreatitis.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Adipose Stem Cell Therapy Mitigates Chronic Pancreatitis via Differentiation into Acinar-like Cells in Mice

et al. 2017

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“…Studies have shown that the transplantation of adipose-derived mesenchymal stem cells (aScs) markedly improves revascularization and tissue perfusion in limb ischemia (17). Various studies have demonstrated that aScs have a treatment effect for severe acute pancreatitis (AP) (18), type 1 diabetes (19,20) and experimental colitis (21) in animal models. aScs are currently being tested in clinical trials for the treatment of transsphincteric cryptoglandular fistulas (22), crohn's-related rectovaginal fistula (23), refractory rheumatoid arthritis (24), severe osteoarthritis of the knee (25), systemic sclerosis (26) and other diseases (27).…”

Section: Introductionmentioning

confidence: 99%

Adipose‑derived mesenchymal stem cells ameliorate dibutyltin dichloride‑induced chronic pancreatitis by inhibiting the PI3K/AKT/mTOR signaling pathway

Sun

et al. 2020

Mol Med Report

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adipose-derived mesenchymal stem cells (aScs) play a positive role in tissue injury repair and regeneration. The aim of this study was to determine whether aScs could ameliorate chronic pancreatitis (cP) induced by the injection of dibutyltin dichloride (dBTc) and to elucidate its potential mechanisms. Furthermore, this study also explored whether there was a significant difference if the ASCs were injected via the inferior vena cava or the left gastric artery. cP was induced in rats by a single intravenous administration of dBTc, and the accumulation of collagen and apoptotic rates of pancreatic acinar cells were analyzed. according to the results, aScs markedly reduced dBTc-induced pancreatic damage and collagen deposition in the rat model of cP. Moreover, aScs significantly decreased pancreatic cell apoptosis by regulating the expression levels of caspase-3, BaX and Bcl-2. These effects were observed regardless of whether the injection was in the inferior vena cava or the left gastric artery. it was also found that the expression levels of phosphorylated Pi3K, aKT and mTor in pancreatic tissues of the dBTc-induced cP model group were significantly increased, while the expression levels of phosphorylated Pi3K, aKT and mTor in the two treatment groups were markedly decreased. aScs noticeably suppressed the Pi3K/aKT/mTor pathway in the pancreatic tissue of dBTc-induced cP. This study indicated that ASCs protect against pancreatic fibrosis by modulating the Pi3K/aKT/mTor pathway, and have the potential to be a new strategy for the treatment of cP in the future.

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“…Mesenchymal stem cell (MSC) therapies are emerging as potential adjunct or single‐agent treatments for a variety of inflammatory and immune‐mediated conditions in veterinary medicine, including osteoarthritis, tendonopathy, spinal cord trauma, keratoconjunctivitis sicca, pancreatitis, inflammatory bowel disease (IBD), and feline gingivostomatitis . In these applications, stem cells are used for their autocrine and paracrine immunomodulatory effects . Adipose‐derived MSC (AD‐MSC) have gained in popularity because of the abundance and ease of harvesting adipose tissue, documented multipotency of the cells, greater self‐renewal capacity, and superior MSC:volume ratio compared to bone marrow‐derived MSC …”

Section: Introductionmentioning

confidence: 99%

Isolation of canine adipose‐derived mesenchymal stem cells from falciform tissue obtained via laparoscopic morcellation: A pilot study

et al. 2019

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Objective To evaluate the feasibility of stem cell isolation from falciform fat harvested via laparoscopic morcellation. Study design Pilot study. Animals Eleven client‐owned dogs. Methods Falciform was harvested traditionally via laparotomy and laparoscopically via tissue morcellation. Harvested tissue was processed with a commercially available adipose tissue dissociation kit to obtain a stromal vascular fraction (SVF). Cells were subsequently labeled for CD90, CD45, and CD44 cell surface antigens by using magnetic‐activated cell sorting (MACS) and fluorescence‐activated cell sorting flow cytometry. CD90+ cells were quantitated, and their viability was assessed with a hemocytometer and a trypan blue exclusion test of cell viability. Results No perioperative complications occurred in dogs undergoing laparoscopic morcellation. Laparoscopically and traditionally harvested samples yielded an average of 0.39 (±0.1) × 106 and 0.33 (±0.1) × 106 CD90+ cells, respectively, per 10 million SVF cells. CD90+ cell viability after MACS was 89% (±11%) for morcellated and 86% (±7%) for traditionally harvested samples. Neither CD90+ cell quantity nor viability was different between samples obtained via traditional laparotomy vs laparoscopic morcellation (P = .38 and P = .63, respectively). Populations of CD90+ cells isolated with each harvest technique had similar CD44 and CD45 expression profiles. Conclusion Viable populations of CD90+ cells with similar CD44/CD45 expression profiles were isolated from laparoscopically morcellated and traditionally harvested falciform tissue. No appreciable morbidity was associated with laparoscopic falciform morcellation. Clinical significance Laparoscopic morcellation is a safe and effective minimally invasive approach to falciform tissue harvest for adipose‐derived mesenchymal stem cell isolation.

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Canine adipose tissue-derived mesenchymal stem cells ameliorate severe acute pancreatitis by regulating T cells in rats

Cited by 30 publications

References 38 publications

Adipose Stem Cell Therapy Mitigates Chronic Pancreatitis via Differentiation into Acinar-like Cells in Mice

Adipose Stem Cell Therapy Mitigates Chronic Pancreatitis via Differentiation into Acinar-like Cells in Mice

Adipose‑derived mesenchymal stem cells ameliorate dibutyltin dichloride‑induced chronic pancreatitis by inhibiting the PI3K/AKT/mTOR signaling pathway

Isolation of canine adipose‐derived mesenchymal stem cells from falciform tissue obtained via laparoscopic morcellation: A pilot study

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