Canine corpus luteum regression: Apoptosis and caspase-3 activity

Luz, Marcelo Rezende; Cesário, Maria Dalva; Binelli, Mário; Lopes, Maria Denise

doi:10.1016/j.theriogenology.2006.02.025

Cited by 16 publications

(15 citation statements)

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“…The final stages of luteal regression were characterised by very small luteal cells and a prevalence of connective tissue as shown for CLs in April (non-pregnant or group B). This final structural luteal regression begins with programmed cell death (apoptosis) and involution of the CL, and culminates in an ovarian scar called corpus albicans (Davis & Rueda 2002, Luz et al 2006, Stocco et al 2007.…”

Section: Discussionmentioning

confidence: 99%

Histological and endocrine characterisation of the annual luteal activity in Eurasian lynx (Lynx lynx)

et al. 2012

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Lynx presents a unique sexual cycle with persistent corpora lutea (CLs) and elevated serum progesterone (P 4 ) throughout parturition and lactation. In other mammals, CLs normally disintegrate after parturition, therefore the aim of our study was to characterise the annual life cycle of lynx CLs. Ovaries from Eurasian lynxes were obtained from the National Veterinary Institute in Sweden, where tissues from killed lynx were stored at K20 8C. Ovaries from 66 animals were weighed; each corpus luteum was segmented for histology and hormone analysis. Ovary and CLs weights were constant throughout the year, peaking during pregnancy. In non-pregnant lynxes, the seasonal level of intraluteal steroids was steady for P 4 (3.2G1.9 S.D. mg/g, nZ53) and total oestrogens (18.3G15.5 S.D. ng/g, nZ53). Within histology slides, structurally intact luteal cells were found throughout the year with the highest incidence in March/April; evidence of luteal regression was predominantly found in post-breeding season. Ovaries from pregnant animals contained two types of CLs. Group A was bigger in size with large luteal cells (P 4 , 72.3G65.4 S.D. mg/g; oestrogen, 454.0G52.4 S.D. ng/g). In contrast, group B were smaller, with greater luteal regression and lower steroid concentrations (P 4 , 8.3G2.9 S.D. mg/g; oestrogen, 31.5G20.4 S.D. ng/g). Our results suggest that structural luteolysis proceeds throughout the year and into next breeding cycle, resulting in two CLs types on the same ovary.

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Section: Discussionmentioning

confidence: 99%

Histological and endocrine characterisation of the annual luteal activity in Eurasian lynx (Lynx lynx)

et al. 2012

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“…The relationship among apoptosis, follicular atresia, and luteolysis has been well documented in mammals (Luz et al 2006;Rolaki et al 2005). In birds, recruited follicles undergo ovulation followed by postovulatory regression including the apoptosis of granulosa cells and the resorption of the follicular remains (Tilly et al 1991;Murdoch et al 2005;Sundaresan et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…However, caspase-independent apoptotic pathways have also been found including those involving mitochondrial apoptosisinducing factor and non-caspase proteases such as cathepsins, granzymes, and endonuclease G, which can induce apoptosis either independently or cooperatively with caspases (Zhang et al 2003). Caspase 3 is expressed in luteal and thecal cells of healthy corpus luteum and in the granulosa of atretic follicles of mammals (Hussein 2005;Luz et al 2006). In P. argenteus, the immunoreactivity for caspase 3 has been identified mainly in the follicular cells of postovulatory follicles.…”

Section: Discussionmentioning

confidence: 99%

“…Pro-apoptotic members of the B-cell lymphoma 2 (Bcl-2) family, tumor necrosis factor (TNF), and caspases are related to follicular atresia, whereas Bcl-2, Bax, the Fas ligand, and caspases are associated with folliculogenesis, and the Fas/Fas ligand, caspase-3, and Bax have been implicated in luteolysis (Antonsson 2001;Hussein 2005;Luz et al 2006). In teleosts, apoptosis is important in the selection and recruitment of follicles for vitellogenesis and in ovarian regression after spawning (Drummond et al 2000;Van Der Kraak 2001, 2002;Santos et al 2005;Thomé et al 2006).…”

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confidence: 99%

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Relationship among follicular apoptosis, integrin β1 and collagen type IV during early ovarian regression in the teleost Prochilodus argenteus after induced spawning

Santos

Sato²,

Moro³

et al. 2008

Cell Tissue Res

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Early ovarian regression was analyzed in the neotropical freshwater teleost, curimatã-pacu (Prochilodus argenteus), in order to evaluate follicular apoptosis, basement membrane morphology, and integrin beta1 and collagen type IV immunostainning in postovulatory follicles. Mature females were induced to spawn by using carp pituitary extract for study of ovarian regression up to 5 days post-spawning. Morphometric analyses showed that the postovulatory follicle area decreased progressively after spawning and was coupled to the gonadosomatic index (r=0.92). During ovarian regression, follicular cells detached from the neighboring cells and basement membrane and then died by apoptosis. The follicular basement membrane became thicker and diffuse and was breached during regression of the postovulatory follicles. Follicular apoptosis was detected by TUNEL, histology, and electron microscopy. The ladder pattern of apoptotic DNA was revealed by agarose gel electrophoresis. The apoptotic index for the follicular cells increased until 3 days post-spawning and decreased thereafter. Immunohistochemistry reactions detected caspase 3, integrin beta1, and collagen type IV in the follicular layer of the postovulatory follicles. Labeling for integrin beta1 and collagen type IV decreased significantly, whereas a peak in cell death occurred 3 days post-spawning. At 4-5 days post-spawning, the connective theca was more thickened and vascularized. Simultaneously, granulocytes migrated toward the follicular lumen. Thus, follicular apoptosis contributes to early ovarian regression in P. argenteus. Additionally, our findings suggest integrin beta1 and collagen type IV as possible survival factors for follicular cells in teleost ovary.

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“…Once peripheral P4 reaches levels below 1 ng/ml, the canine reproductive cycle, per defi nition, enters anestrus (see review Kowalewski 2012Kowalewski , 2014. In contrast to the actively regulated prepartum luteolysis, which is associated with strong apoptotic signals occurring concomitantly with the prepartum PGF2α increase, during the extended luteal regression only sporadic apoptotic signals can be observed (Hoffmann et al 2004b ;Luz et al 2006 ;Kowalewski 2014 ).…”

Section: Endocrine Patterns During Pregnancy and Pseudopregnancymentioning

confidence: 99%

The Dog: Nonconformist, Not Only in Maternal Recognition Signaling

Kowalewski

Gram

Kautz

et al. 2015

Regulation of Implantation and Establishment of Pregnancy in Mammals

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Although similar at the molecular and cellular levels, endocrine mechanisms governing reproductive function in the domestic dog (Canis familiaris) differ markedly at the regulatory level from those known in other domestic animal species. Some of the events, e.g., the lack of luteolysis in the absence of pregnancy, resulting in similar luteal function and, therefore, hormonal profiles in early pregnant and nonpregnant animals, are species-specific. Consequently, no early gestation marker has so far been identified for the dog. Following implantation, relaxin of fetal placental origin can be detected and used for pregnancy diagnosis. Characterized by the lack of an active luteolytic principle from intra- or extra-luteal sources, the canine reproductive cycle appears to represent a "basic" form of mammalian reproductive function with apparently reduced opportunities for facilitating fecundity and hastening reproduction. Nevertheless, in the dog some kind of mechanism for synchronization between blastocyst development and uterine preparation for pregnancy must have evolved in order to support gestation. Driven by this assumption, studies including our recent investigations have been initiated aimed at characterizing some of the embryo-mediated effects of the preimplantation embryo on the canine uterus. Moreover, the lack of a uterine luteolysin and consequently the absence of a need to develop an antiluteolytic strategy make the dog an interesting model for investigating early evolutionary mechanisms involved in the preparation for implantation and ensuring embryo survival. These mechanisms result in an inverse relationship between the duration of pregnancy and of the nonpregnant cycle in the dog, compared with all other domestic animal species.

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Canine corpus luteum regression: Apoptosis and caspase-3 activity

Cited by 16 publications

References 16 publications

Histological and endocrine characterisation of the annual luteal activity in Eurasian lynx (Lynx lynx)

Histological and endocrine characterisation of the annual luteal activity in Eurasian lynx (Lynx lynx)

Relationship among follicular apoptosis, integrin β1 and collagen type IV during early ovarian regression in the teleost Prochilodus argenteus after induced spawning

The Dog: Nonconformist, Not Only in Maternal Recognition Signaling

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