Canine Ehrlichiosis Caused Simultaneously by <i>Ehrlichia canis</i> and <i>Ehrlichia platys</i>

Pan, Hua; Ma, Yue; Tong, Shide; Sun, Ying; Wen, Bin; Xiangrui, C.

doi:10.1111/j.1348-0421.2000.tb02557.x

Cited by 45 publications

(25 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Co-infecções por A. platys e E. canis têm sido relatadas em diferentes regiões do mundo, como China (HUA et al, 2000), Caribe (YABSLEY et al, 2008), Venezuela (SUKSAWAT et al, 2001) e Brasil (SANTOS et al, 2009 A. platys e E. canis podem ser transmitidos pelo mesmo vetor (HUANG et al, 2005). O principal vetor de E. canis para cães é o carrapato Rhipicephalus sanguineus (LEWIS et al, 1977) e, embora a transmissão experimental de A. platys por este carrapato tenha falhado (SIMPSON et al, 1991), ele é apontado como o principal transmissor para cães em diversas regiões do mundo (SANOGO et al, 2003;YABSLEY et al, 2008).…”

Section: Resultsunclassified

ComparaÇão de nested-PCR com o diagnóstico direto na detecÇão de Ehrlichia canis e Anaplasma platys em cães

Ramos

Ramos²,

Araújo³

et al. 2009

RBPV

View full text Add to dashboard Cite

ResumoOs sinais clínicos das infecções por Ehrlichia canis e Anaplasma platys são similares, e o diagnóstico desses patógenos feito por esfregaços sanguíneos corados é difícil devido à sensibilidade e especificidade. Por outro lado, os diagnósticos moleculares são altamente sensíveis e específicos, e nested-PCRs têm sido otimizadas para o diagnóstico preciso desses patógenos em cães. Em um Hospital Veterinário Escola, amostras de sangue total com EDTA foram obtidas de 100 cães, e esfregaços foram feitos das amostras de sangue para busca dos parasitos intracelulares. Para cada amostra, DNA foi extraído e submetido à nPCR para detecção de E. canis e A. platys. Os resultados dos esfregaços sanguíneos mostraram que 9% dos animais foram positivos para E. canis e 21% para A. platys. Com relação à nPCR, 57 e 55% dos cães foram positivos para E. canis e A. platys, respectivamente. Quando comparados com a nPCR, os esfregaços sanguíneos corados revelaram resultados falso-negativos para E. canis e A. platys. Os resultados indicam que a nPCR é altamente sensível e específica para detecção de ambos os patógenos, e os diagnósticos moleculares podem ser mais úteis nos Hospitais Veterinários.Palavras-chave: nPCR, cães, Ehrlichia, Anaplasma. AbstractThe clinical signs of Ehrlichia canis and Anaplasma platys infection are similar, and the diagnosis of these pathogens made by stained blood smears is poor due sensibility and specificity. On the other hand, the molecular diagnosis is highly sensitive and specific and nested-PCR have been optimized for accurate diagnosis these pathogens in dogs. At the veterinary teaching hospital, whole-blood samples with EDTA were obtained from 100 dogs and smears were made from blood samples for evaluation for intracellular parasites. For each sample, DNA was extracted and submitted to nPCR analysis for detection of E. canis and A. platys. The results of stained blood smears showed 9% of the animals were positive for E. canis and 21% for A. platys. Regarding of nPCR analysis, 57 and 55% of dogs were positive for E. canis and A. platys respectively. As compared to a nested PCR, the stained blood smears revealed false-negative results for both E. canis and A. platys. The results indicate that the nPCR is highly sensitive and specific for detection of both pathogens and the molecular diagnosis could be more useful at veterinary hospital.

show abstract

Section: Resultsunclassified

ComparaÇão de nested-PCR com o diagnóstico direto na detecÇão de Ehrlichia canis e Anaplasma platys em cães

Ramos

Ramos²,

Araújo³

et al. 2009

RBPV

View full text Add to dashboard Cite

show abstract

“…Co-infections by A. platys and E. canis have been reported in several regions of Brazil such as Ribeirão Preto, São Paulo (SANTOS et al, 2009), andCuiabá (SOUSA et al, 2009), as well as other countries such as China (HUA et al, 2000) and Venezuela (SUKSAWAT et al, 2001). Simultaneous infections by Babesia canis vogeli and Anaplasma platys were observed in Australia by Brown et al (2006).…”

Section: Resultsmentioning

confidence: 99%

Parasitological and molecular detection of Babesia canis vogeli in dogs of Recife, Pernambuco and evaluation of risk factors associated

et al. 2016

View full text Add to dashboard Cite

This work aims to detect the presence of Babesia canis vogeli in dogs from Recife, Pernambuco via molecular and parasitological detection methods, and to assess the risk factors associated with this parasite. A total of 146 dogs (male and female) of varying breeds and ages that presented clinical symptoms of babesiosis were assessed at a clinical care center in the Veterinary School Hospital. Blood was obtained via venopuncture for hemoparasite detection and polymerase chain reaction (PCR). Using a commercial kit, DNA was extracted from blood samples. For the PCR reaction, an approximately 590 base pair long genetic sequence was used to detect the presence of B. canis vogeli. The forward primer, denoted as BAB1 (5'-GTG AAC CTT ATC ACT TAA AGG-3'), was specific for a conserved region on the 18S rRNA gene of Babesia spp., and the antisense primer was denoted as BAB4 (5'-CAA CTC CTC CAC GCA ATC G-3'). PCR results suggested that the percentage of Babesia canis vogeli infection was 4.8%. Through descriptive statistical analysis of the data, we observed that there was higher frequency of parasite infection associated with male dogs above two years of age, with a defined breed, from the countryside, are domiciled, and also suffer from tick infestation. We conclude that regardless of the type of risk factor, babesiosis can be found throughout Recife, Pernambuco, and its prevalence does not vary in most regions of Brazil. Our results indicate that PCR is a sensitive test for the detection of blood parasites, and should be performed as a clinical routine.

show abstract

“…vogeli antibodies, and 4 (5.1%) had antibodies for both hemoparasites, indicating that, although it is a characteristic finding, thrombocytopenia is not exclusive to canine ehrlichiosis and may be present in different diseases (18) . Thus, those dogs seronegative to E. canis and B. vogeli, but with values for hematological parameters and/or the platelet count that are low for canines, are likely infected by other hemoparasites, such as Anaplasma platys and Hepatozoon canis, which are also transmitted by the tick vector R. sanguineus (33,34) .…”

Section: Resultsmentioning

confidence: 99%

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

et al. 2017

View full text Add to dashboard Cite

The aim of this study was to determine the prevalence of anti-Ehrlichia canis and anti-Babesia vogeli IgG antibodies in dogs and correlate this prevalence with risk factors to evaluate the relation of serological status to hematological findings. Blood samples of dogs attended from September 2011 to March 2012 at the veterinary hospital of the Federal University of Lavras, Brazil, were analyzed using an indirect fluorescence antibody test (IFAT). Of the total 160 dog serum samples, 23.7% (38 dogs; CI 95 17.7% -30.7%) were seropositive for E. canis, 40.0% (64 dogs; CI 95 40.0% -59.2%) for B. vogeli, and 5.6% (9 dogs) for both hemoparasites. None of the epidemiological variables showed a significant association (P>0.05) with seropositivity to E. canis and B. vogeli. Dogs seropositive for E. canis showed lower values for hematocrit (P<0.05). However, for the erythrogram, the platelet count, the leukogram and clinical signs, no significant difference (P>0.05) was observed between dogs that were seropositive and seronegative for E. canis or for B. vogeli. Serological results suggest that infection with E. canis and B. vogeli is endemic in the canine population in question, with a prevalence of the subclinical phase (asymptomatic) in dogs that are seropositive for ehrlichiosis or babesiosis. Keywords: babesiosis; canine; ehrlichiosis; IFAT; laboratory findings. ResumoO objetivo deste estudo foi determinar a prevalência de anticorpos IgG anti-Ehrlichia canis e antiBabesia vogeli em cães e correlacionar com a prevalência e fatores de risco para avaliar a relação do estado sorológico com os achados hematológicos. Amostras de sangue de cães coletadas de setembro de 2011 a março de 2012, no Hospital Veterinário da Universidade Federal de Lavras, Brasil, foram analisadas usando o teste de reação de imunofluorescência indireta (RIFI). Do total de amostras de soro de 160 cães, 23,7% (38 cães; IC95 17,7% -30,7%) foram soropositivos para E. canis, 40,0% (64 cães; IC95 40,0% -59,2%) para B. vogeli, e 5,6% (9 cães) para os dois hemoparasitas. Nenhuma das variáveis epidemiológicas mostrou associação significativa (P> 0,05) com a soropositividade para E. canis e B. vogeli. Cães soropositivos para E. canis mostraram valores médios mais baixos para hematócrito (P<0,05). No entanto, para o eritrograma, a contagem de plaquetas, o leucograma e os sinais clínicos, nenhuma diferença significativa (P> 0,05) foi observada entre cães soropositivos e soronegativos para E. canis ou para B. vogeli. Os resultados sorológicos deste estudo sugerem que a Cienc. anim. bras., Goiânia, v.18, 1-9, e-36095, 2017 2 FONSECA, J.P., et al.infecção por E. canis e B. vogeli é endêmica na população canina em questão, com uma prevalência da fase subclínica (assintomática) em cães soropositivos para erliquiose ou babesiose.

show abstract

Canine Ehrlichiosis Caused Simultaneously by Ehrlichia canis and Ehrlichia platys

Cited by 45 publications

References 8 publications

ComparaÇão de nested-PCR com o diagnóstico direto na detecÇão de Ehrlichia canis e Anaplasma platys em cães

ComparaÇão de nested-PCR com o diagnóstico direto na detecÇão de Ehrlichia canis e Anaplasma platys em cães

Parasitological and molecular detection of Babesia canis vogeli in dogs of Recife, Pernambuco and evaluation of risk factors associated

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

Contact Info

Product

Resources

About