Canine-Origin Platelet-Rich Fibrin as an Effective Biomaterial for Wound Healing in Domestic Cats: A Preliminary Study

Abstract

Platelet-rich fibrin (PRF) is a recent platelet-based biomaterial, poised as an innovative regenerative strategy for the treatment of wounds from different etiologies. PRF is defined as a biodegradable scaffold containing elevated amounts of platelets and leukocytes having the capability to release high concentrations of bioactive structural proteins and acting as a temporal release healing hemoderivative. This study aimed to evaluate the performance of canine-origin PRF, obtained from blood of screened donors, as a regenerative biomaterial suitable for the treatment of critical wounds in felines. Four short-hair felines with naturally occurring wounds were enrolled in this study. Three of the wounds were considered infected. Each PRF treatment was the result of the grafting of newly produced PRFs at the recipient area. The PRF treatment was initially performed two to three times per week, followed by single weekly treatments. The study was finalized when complete wound closure was achieved. No topical antimicrobial/antiseptic treatment was applied. The present research demonstrated that xenogenic PRFs significantly induced healthy vascularized granulation tissue in lesions with soft tissue deficit, also prompting the epithelization at the injured site. No rejection, necrosis, or infection signs were recorded. Additionally, PRF-therapy was revealed to be a biological cost-effective treatment, accelerating the wound healing process.

Autologous platelet-rich fibrin promotes wound healing in cats

“…Single use of platelet concentrates may not provide satisfactory clinical results, particular in infected wounds with high demands on healing processes (44). Repeated application of PRF has been employed in a previous study with heterologous canine PRF to manage wounds in compromised felines, with the intent to increase in-site concentration of cytokines and GFs (23). As such, we decided to have two applications of PRF per cat for our treatment protocol.…”

“…This clinical success, in addition to other appealing properties of being autologous, and simple and inexpensive to produce, has led to an interest in using PRF in veterinary medicine (19,20). Early, limited studies have examined autologous PRF use in donkeys (21) and dogs (22), with one preliminary study using heterologous canine-origin PRF in four cats with naturally occurring wounds (23).…”

Effects of Autologous Platelet-Rich Fibrin (PRF) Therapy on Wound Healing in Dogs

“…Protocol for the production of the PRFs Each PRF clot was using an aseptic technique as previous described (14,18,20). Brie y, peripheric venous blood was collected to sterilized conical base polypropylene tubes (57 x 15.3 mm), without clot activator.…”

“…[(Wound Area at day 1 − Wound Area at Speci c Time point) / Wound Area at day 1] × 100 (20). Results were expressed as the median, and interquartile ranges (IQR).…”

In Vitro Assessment of Lyophilized Advanced Platelet-Rich Fibrin from Dogs in Promotion of Growth Factor Release and Wound Healing

“…Both in vitro and in vivo research have supported PRF as a biomaterial strategy for the treatment of a wide variety of types of wounds in humans and animals [ 9 , 25 , 26 ]. Recently, information on the application of canine PRF to wound healing has been encouraged for veterinary use [ 27 , 28 ]. However, the application of PRF remains reliant upon fresh preparations due to the short half-life of the product [ 29 ].…”