Cannabinoid receptor gene detection by electrochemical genosensor

Berti, Francesca; Eisenkolbl, Claudia; Minocci, D.; Nieri, Paola; Rossi, Anna Maria; Mascini, Marco; Marrazza, Giovanna

doi:10.1016/j.jelechem.2011.01.021

Cited by 14 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a different approach, Berti et al [121] combined the remarkable properties of carbon nanotubes with the high stability of LNA probes. The resulting biosensor was applied to the detection of a PCR amplicon belonging to a region of the CB2 cannabinoid receptor gene.…”

Section: Lna-based Biosensorsmentioning

confidence: 99%

Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development

2012

View full text Add to dashboard Cite

Nucleic acid biosensors have a growing number of applications in genetics and biomedicine. This contribution is a critical review of the current state of the art concerning the use of nucleic acid analogues, in particular peptide nucleic acids (PNA) and locked nucleic acids (LNA), for the development of high-performance affinity biosensors. Both PNA and LNA have outstanding affinity for natural nucleic acids, and the destabilizing effect of base mismatches in PNA- or LNA-containing heterodimers is much higher than in double-stranded DNA or RNA. Therefore, PNA- and LNA-based biosensors have unprecedented sensitivity and specificity, with special applicability in DNA genotyping. Herein, the most relevant PNA- and LNA-based biosensors are presented, and their advantages and their current limitations are discussed. Some of the reviewed technology, while promising, still needs to bridge the gap between experimental status and the harder reality of biotechnological or biomedical applications.

show abstract

Section: Lna-based Biosensorsmentioning

confidence: 99%

Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development

2012

View full text Add to dashboard Cite

show abstract

“…For its specific detection and good stability, LNA was also used in electrochemical biosensors 91,92 and carbon nanotube biosensors 93.…”

Section: Xna Molecular Beacons For Nucleic Acidsmentioning

confidence: 99%

Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid

Wang¹,

Chen²,

Long³

et al. 2013

Theranostics

View full text Add to dashboard Cite

Molecular beacons (MBs) of DNA and RNA have aroused increasing interest because they allow a continuous readout, excellent spatial and temporal resolution to observe in real time. This kind of dual-labeled oligonucleotide probes can differentiate between bound and unbound DNA/RNA in homogenous hybridization with a high signal-to-background ratio in living cells. This review briefly summarizes the different unnatural sugar backbones of oligonucleotides combined with fluorophores that have been employed to sense DNA/RNA. With different probes, we epitomize the fundamental understanding of driving forces and these recognition processes. Moreover, we will introduce a few novel and attractive emerging applications and discuss their advantages and disadvantages. We also highlight several perspective probes in the application of cancer therapeutics.

show abstract

“…10,11 Numerous detection methods have been proposed for SNP genotyping, such as enzyme-mediated SNP detection assays, TaqMan assay, allelespecific polymerase chain reaction (PCR), endonuclease cleavage, microarray-based screening, fluorescence resonance energy transfer (FRET)-based assays, colorimetric assays and electrochemical biosensors. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] As a relatively mature high throughput analytical method, the oligonucleotide microarray technology is able to screen hundreds to thousands of different SNP events in one experiment with minuscule consumption of analytes and reagents. [28][29][30][31][32] During the last two decades, gold nanoparticles (GNPs) have been extensively employed for the development of ultrasensitive detection and imaging methods in analytical or biological sciences because GNPs have unique optical properties (i.e.…”

Section: Introductionmentioning

confidence: 99%

Multiple detection of single nucleotide polymorphism by microarray-based resonance light scattering assay with enlarged gold nanoparticle probes

Gao

Lei

2016

Analyst

View full text Add to dashboard Cite

The mapping of specific single nucleotide polymorphisms (SNPs) in patients' genome is a critical process for the development of personalized therapy. In this work, a DNA microarray-based resonance light scattering (RLS) assay has been developed for multiplexed detection of breast cancer related SNPs with high sensitivity and selectivity. After hybridization of the desired target single-stranded DNAs (ssDNAs) with the ssDNA probes on a microarray, the polyvalent ssDNA modified 13 nm gold nanoparticles (GNPs) are employed to label the hybridization reaction through the formation of a three-stranded DNA system. The H2O2-mediated enlargement of GNPs is then used to enhance the RLS signal. The microarray-based RLS assay provides a detection limit of 10 pM (S/N = 3) for the target ssDNA and determines an allele frequency as low as 1.0% in the target ssDNA cocktail. Combined with an asymmetric PCR technique, the proposed assay shows good accuracy and sensitivity in profiling 4 SNPs related to breast cancer of three selected cell lines.

show abstract

Cannabinoid receptor gene detection by electrochemical genosensor

Cited by 14 publications

References 34 publications

Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development

Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development

Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid

Multiple detection of single nucleotide polymorphism by microarray-based resonance light scattering assay with enlarged gold nanoparticle probes

Contact Info

Product

Resources

About