Cannabinoids vs. whole metabolome: Relevance of cannabinomics in analyzing Cannabis varieties

Vásquez-Ocmín, Pedro; Marti, Guillaume; Bonhomme, Maxime; Mathis, Fabienne; Fournier, Sylvie; Bertani, Stéphane; Maciuk, Alexandre

doi:10.1016/j.aca.2021.339020

Cited by 26 publications

(13 citation statements)

References 64 publications

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“…‘Earlina 8FC’ and ‘S. Jubileu’ are a little recognized variety among 600 Cannabis cultivars [ 50 , 51 ]. They are used industrially for vegetable oil production; however, there is little information about these varieties in the literature.…”

Section: Methodsmentioning

confidence: 99%

Quality of Oil Pressed from Hemp Seed Varieties: ‘Earlina 8FC’, ‘Secuieni Jubileu’ and ‘Finola’

et al. 2022

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In the last decade, the demand for edible niche oils has increased. Therefore, the aim of this study was to characterize the seeds hemp (Cannabis sativa L.) varieties: ‘Finola’ (FIN-314)’, ‘Earlina 8FC’, and ‘Secuieni Jubileu’, and cold and hot pressed oils were prepared from each seed. The seeds were examined for moisture content, granulometric distribution, bulk density, and fat content. Seeds were pressed without and with preconditioning (60 °C), and oil yield and pressing time were recorded. The oil was filtered through cellulose membranes. Oil–water content, oil color, fatty acid profile, and sterol content were studied. From the study conducted, there are significant differences in the parameters of oil recovery and its quality compared to ‘Finola’ seed oil, which is widely reported in the literature. ‘Finola’ oil yield was the lowest, with an average of 79% compared to ‘Earlina’ (82%) and ‘S. Jubileu’ (84%). All oil samples contained a comparable amount of sterols, with campesterol (0.32 mg/g), β-sitosterol (1.3 mg/g) and Δ5-avenasterol (0.15 mg/g) predominating. From the organoleptic evaluation, it was evident that both varieties hemp oils and marc (‘Earlina’ and ‘S. Jubileu’) were not bitter like the “Finola” oil and marc. More detailed studies in this direction have to be undertaken.

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Section: Methodsmentioning

confidence: 99%

Quality of Oil Pressed from Hemp Seed Varieties: ‘Earlina 8FC’, ‘Secuieni Jubileu’ and ‘Finola’

et al. 2022

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“…Despite their potential to enhance the food basket, the chemodiversity of pseudocereals has received little attention so far. To explore this chemodiversity, we used an untargeted metabolomics approach referred to as “metabotyping” as we did in a previous work investigating Cannabis cultivars (Vásquez-Ocmín et al, 2021). We thus analyzed the whole metabolomes of several pseudocereal grains collected in the Andes of Peru, as well as one quinoa sample from France ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…LC-MS data were processed according to the MSCleanR workflow (Fraisier-Vannier et al, 2020; Vásquez-Ocmín et al, 2021). Briefly, batches in PI and NI were processed separately with MS-DIAL version 4.80 (Tsugawa, Cajka, et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

“…Metabolomics based on technology like hyphenated liquid chromatography-mass spectrometry (LC-MS) assisted by bioinformatics and statistical analysis is a powerful approach for both the detection and the annotation of key compounds, as well as for deciphering their biological functions. Our team has previously implemented a workflow based on untargeted LC-MS-based metabolomics able to achieve the metabotyping of plants by identifying their main biomarkers (Vásquez-Ocmín et al, 2021). In the present study, we applied this approach to both achieve a comprehensive chemical mapping of pseudocereal samples collected in the Andes, and to classify them according to their chemotype.…”

Section: Introductionmentioning

confidence: 99%

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Metabotyping of Andean pseudocereals and characterization of emerging mycotoxins

Vásquez-Ocmín

Marti

Gadea

et al. 2022

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Pseudocereals are best known for three crops derived from the Andes: quinoa (Chenopodium quinoa, Chenopods I), canihua (C. pallidicaule, Chenopods I), and kiwicha (Amaranthus caudatus). Their grains are recognized for their nutritional benefits; however, there is a higher level of polyphenism and the chemical foundation that would rely with such polyphenism has not been thoroughly investigated. Meanwhile, the chemical food safety of pseudocereals remains poorly documented. Here we applied untargeted and targeted metabolomics approach by LC-MS to achieve both: i) a comprehensive chemical mapping of pseudocereal samples collected in the Andes to classify them according to their chemotype; ii) a quantification of their contents in emerging mycotoxins. An inventory of the fungal community was also realized with the aims to better know the filamentous fungi present in these grains and try to parallel this information with the presence of the molecules produced, especially mycotoxins. Metabotyping permitted to add new insights into the chemotaxonomy of pseudocereals, confirming the previously established phylotranscriptomic clades: Chenopods I (clusters quinoa and canihua), and Amaranthaceae s.s. (cluster kiwicha). Moreover, we report for the first time the presence of mycotoxins in pseudocereals. Sixteen samples of Peru (out of 27) and one sample from France (out of one) were contaminated with Beauvericin, an emerging mycotoxin. There were several mycotoxigenic fungi detected, including Aspergillus sp., Penicillium sp., and Alternaria sp., but not Fusaria.

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“…The recent advance in metabolomics including application devices of liquid or gas chromatography-coupled with mass spectrometry (LC- or GC-MS) or nuclear magnetic resonance spectroscopy (NMR) together with bioinformatic and chemometric approaches has prompted the understanding of the metabolite profiling of cannabis plants and related species [ 1 , 2 , 3 ]. More than 500 constituents have recently been elucidated in the cannabis species, including terpenes (mono-, di-, sesqui-and triterpenes), flavonoids, alkanes, sugars, nitrogenous compounds (such as spermidine alkaloids or muscarine), non-cannabinoid phenols, phenylpropanoids, steroids, fatty acids, and other compounds like stilbenes, lignans and phytosterols [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Mass Spectrometry-Based Metabolomics of Phytocannabinoids from Non-Cannabis Plant Origins

et al. 2022

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Phytocannabinoids are isoprenylated resorcinyl polyketides produced mostly in glandular trichomes of Cannabis sativa L. These discoveries led to the identification of cannabinoid receptors, which modulate psychotropic and pharmacological reactions and are found primarily in the human central nervous system. As a result of the biogenetic process, aliphatic ketide phytocannabinoids are exclusively found in the cannabis species and have a limited natural distribution, whereas phenethyl-type phytocannabinoids are present in higher plants, liverworts, and fungi. The development of cannabinomics has uncovered evidence of new sources containing various phytocannabinoid derivatives. Phytocannabinoids have been isolated as artifacts from their carboxylated forms (pre-cannabinoids or acidic cannabinoids) from plant sources. In this review, the overview of the phytocannabinoid biosynthesis is presented. Different non-cannabis plant sources are described either from those belonging to the angiosperm species and bryophytes, together with their metabolomic structures. Lastly, we discuss the legal framework for the ingestion of these biological materials which currently receive the attention as a legal high.

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Cannabinoids vs. whole metabolome: Relevance of cannabinomics in analyzing Cannabis varieties

Cited by 26 publications

References 64 publications

Quality of Oil Pressed from Hemp Seed Varieties: ‘Earlina 8FC’, ‘Secuieni Jubileu’ and ‘Finola’

Quality of Oil Pressed from Hemp Seed Varieties: ‘Earlina 8FC’, ‘Secuieni Jubileu’ and ‘Finola’

Metabotyping of Andean pseudocereals and characterization of emerging mycotoxins

Mass Spectrometry-Based Metabolomics of Phytocannabinoids from Non-Cannabis Plant Origins

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