Cannabis sativa CBD Extract Shows Promising Antibacterial Activity against Salmonella typhimurium and S. newington

Abstract

Products derived from Cannabis sativa L. have gained increased interest and popularity. As these products become common amongst the public, the health and potential therapeutic values associated with hemp have become a premier focus of research. While the psychoactive and medicinal properties of Cannabis products have been extensively highlighted in the literature, the antibacterial properties of cannabidiol (CBD) have not been explored in depth. This research serves to examine the antibacterial potential of CBD against Salmonella newington and S. typhimurium. In this study, we observed bacterial response to CBD exposure through biological assays, bacterial kinetics, and fluorescence microscopy. Additionally, comparative studies between CBD and ampicillin were conducted against S. typhimurium and S. newington to determine comparative efficacy. Furthermore, we observed potential resistance development of our Salmonella spp. against CBD treatment.

The Antimicrobial Properties of Cannabis and Cannabis-Derived Compounds and Relevance to CB2-Targeted Neurodegenerative Therapeutics

“…Gildea et al have reported that CBD is active against Salmonella newington as well as S . Typhimurium [ 44 ]. The phytocannabinoid, cannabichromenic acid (CBCA), has been reported to exert potent antibacterial activity against clinical strains of Enterobacter faecalis and both methicillin-resistant and sensitive strains of S. aureus .…”

<em>Cannabis sativa</em> CBD Extract Exhibits Synergy with Broad-Spectrum Antibiotics against <em>Salmonella typhimurium</em>

“…Recent studies have observed CBD's antibacterial activity against several clinically relevant pathogens including Staphylococcus aureus, Streptococcus pneumoniae, Clostridium difficile, Neisseria spp., Moraxella catarrhalis, Legionella pneumophila, and Salmonella spp. [13][14][15][16][17]. With the increased prevalence and occurrence of antimicrobial resistance posing a significant threat to public health, the development of novel antibacterial agents is a necessity [18][19][20][21].…”

“…While many notable resistant bacteria are results of hospital-acquired infections, the threat of resistant foodborne bacteria is a significant threat. Salmonella species are one of the most common and prevalent foodborne pathogens worldwide, found in several food products including poultry, seafood, and other fresh or processed meats [15,21,[30][31][32][33]. Accounting for 1.35 million infections, 26,500 hospitalizations, and 420 deaths annually in the United States alone, Salmonella species have a significant impact on public health [22].…”

“…Accounting for 1.35 million infections, 26,500 hospitalizations, and 420 deaths annually in the United States alone, Salmonella species have a significant impact on public health [22]. The mass and often inappropriate use of antibiotics, especially in the food industry, is a major concern and could lead to mass exposure of the public to antibiotic and multidrug resistance pathogens [15,[18][19][20][21]34,35]. S. typhimurium, the strain of focus in this study, is notable for its ability to develop resistance to antibiotics.…”

Proteolytic Analysis of Epsilon 34 Phage Tailspike protein indicates Partial Sensitivity to Proteinase K

“…Salmonella has been demonstrated to cause several acute and chronic infections in humans [1] . These bacteria is known to be responsible for over 1.2 million illnesses in the US each year [2, 3, 4, 5] . Salmonella species are prone to acquiring resistance to various classes of antibiotics this sparks the need to look for methods of treating salmonella related infections other than the use of antibiotics, thus calling for the application of bacteriophages to manage, control and treat bacterial infections [6, 7] .…”