Canonical Wnt signaling differently modulates osteogenic differentiation of mesenchymal stem cells derived from bone marrow and from periodontal ligament under inflammatory conditions

Liu, Wenjia; Konermann, Anna; Guo, Tao; Jäger, Andreas; Zhang, L; Jin, Yan

doi:10.1016/j.bbagen.2013.11.003

Cited by 96 publications

(91 citation statements)

References 32 publications

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“…For osteogenesis assays, HPDLSCs and PPDLSCs were exposed to different magnitudes of SMS for 12 h, and after reaching 80% confluence, HPDLSCs and PPDLSCs were cultured in osteogenic medium for 7–21 days and prepared for the differentiation analysis as previously described [21]. Alkaline phosphatase (ALP) staining was performed using the BCIP/NBT Alkaline Phosphatase Color Development Kit (Beyotime, Shanghai, China) at day 7, and ALP activity was determined using an Alkaline Phosphatase (AKP/ALP) Detection Kit (Jiancheng Bioengineering, Nanjing, China) at day 7.…”

Section: Methodsmentioning

confidence: 99%

Periodontal Ligament Stem Cells in the Periodontitis Microenvironment Are Sensitive to Static Mechanical Strain

Liu

et al. 2017

Stem Cells International

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During orthodontic treatment, periodontium remodeling of periodontitis patients under mechanical force was abnormal. We have previously confirmed the function impairment of periodontal ligament stem cells (PDLSCs) in the periodontitis microenvironment which might be involved in this pathological process. However, the response of PDLSCs in periodontitis microenvironment to mechanical force remains unclear. Therefore, in the present study, we introduced a Flexcell tension apparatus and investigated the response of PDLSCs obtained from periodontal tissues of periodontitis patients (PPDLSCs) and of those obtained from healthy periodontal tissues (HPDLSCs) to different magnitudes of static mechanical strain (SMS). PPDLSCs showed increased proliferation, decreased osteogenic activity, activated osteoclastogenesis, and greater secretion of inflammatory cytokines. Different magnitudes of SMS exerted distinct effects on HPDLSCs and PPDLSCs. An SMS of 12% induced optimal effects in HPDLSCs, including the highest proliferation, the best osteogenic ability, the lowest osteoclastogenesis, and the lowest secretion of inflammatory cytokines, while the optimal SMS for PPDLSCs was 8%. Excessive SMS damaged PPDLSCs function, including decreased proliferation, an imbalance between osteogenesis and osteoclastogenesis, and an activated inflammatory response. Our data suggest that PPDLSCs are more sensitive and less tolerant to SMS, and this may explain why mechanical force results in undesirable effects in periodontitis patients.

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Section: Methodsmentioning

confidence: 99%

Periodontal Ligament Stem Cells in the Periodontitis Microenvironment Are Sensitive to Static Mechanical Strain

Liu

et al. 2017

Stem Cells International

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“…Although TNF-α-induced inflammation inhibited osteogenic differentiation, PDLSC osteogenic potential was restored by blocking Wnt via use of recombinant Dkk-1 [37] . Likewise, under a chronic inflammatory microenvironment, PDLSC showed reduced Runx2 expression and enhanced NF-kB activity in comparison to BMSC [37,38] . PDLSC have also been implicated in the formation of cementum, one of the three mineralized substances of the tooth.…”

Section: Profiling and Identitymentioning

confidence: 99%

“…Inhibition of Notch signaling in PDLSC, via treatment with γ-secretase inhibitor (a protease that cleaves the Notch protein), showed decreased osteogenic differentiation [35] . In addition, the canonical Wnt signaling pathway, which typically regulates bone homeostasis, has been shown to modulate PDLSC osteogenesis [36,37] . Although TNF-α-induced inflammation inhibited osteogenic differentiation, PDLSC osteogenic potential was restored by blocking Wnt via use of recombinant Dkk-1 [37] .…”

Section: Profiling and Identitymentioning

confidence: 99%

“…In addition, the canonical Wnt signaling pathway, which typically regulates bone homeostasis, has been shown to modulate PDLSC osteogenesis [36,37] . Although TNF-α-induced inflammation inhibited osteogenic differentiation, PDLSC osteogenic potential was restored by blocking Wnt via use of recombinant Dkk-1 [37] . Likewise, under a chronic inflammatory microenvironment, PDLSC showed reduced Runx2 expression and enhanced NF-kB activity in comparison to BMSC [37,38] .…”

Section: Profiling and Identitymentioning

confidence: 99%

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Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

Nguyen

James

et al. 2015

International Journal of Orthopaedics

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Tissue specific mesenchymal stem cells are of great scientific interest across diverse fields in stem cell biology and medicine. Sources of mesenchymal stem cells (MSC) in the craniomaxillofacial skeleton are numerous and are of significant interest to craniofacial and oral surgeons, among other medical and dental specialties. Mesenchymal stem cells are defined by their characteristic cell surface marker expression, multipotentiality and ability for self-renewal. Bone marrow derived MSC (BMSC) are most commonly studied, but are of low abundance in the craniomaxillofacial (CMF) skeleton. Alternative CMF sources of MSC are diverse and include dental pulpal stem cells (DPSC), periodontal ligament stem cells (PDLSC), suture-derived mesenchymal stromal cells (SMC), gingival mesenchymal stem cells (GMSC)and adipose-derived MSC (ASC). This brief review will introduce various MSC sources derived from the head and neck, along with a discussion of their identity, characteristics and results of preclinical studies in tissue engineering.

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“…Native bone marrow and periosteum play an essential role in this process by providing the grafted area with osteogenic elements and a vascular supply. Many authors have shown that bone marrow contains a population of pluripotent mesenchymal stem cells, able to commit into bone-forming elements [234]. Pluripotent mesenchymal cells from the inner layer of periosteum may also migrate to the grafted area and participate in forming a basophilic collagen matrix, which is progressively vascularized and substituted with new bone [56].…”

Section: Introductionmentioning

confidence: 99%

“Over-inlay” block graft and differential morphometry: a novel block graft model to study bone regeneration and host-to-graft interfaces in rats

Ghiacci

Graiani

Ravanetti

et al. 2016

J Periodontal Implant Sci

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PurposeThe aim of this study was to present new a model that allows the study of the bone healing process, with an emphasis on the biological behavior of different graft-to-host interfaces. A standardized “over-inlay” surgical technique combined with a differential histomorphometric analysis is presented in order to optimize the use of critical-size calvarial defects in pre-clinical testing.MethodsCritical-size defects were created into the parietal bone of 8 male Wistar rats. Deproteinized bovine bone (DBBM) blocks were inserted into the defects, so that part of the block was included within the calvarial thickness and part exceeded the calvarial height (an “over-inlay” graft). All animals were sacrificed at 1 or 3 months. Histomorphometric and immunohistochemical evaluation was carried out within distinct regions of interest (ROIs): the areas adjacent to the native bone (BA), the periosteal area (PA) and the central area (CA).ResultsThe animals healed without complications. Differential morphometry allowed the examination of the tissue composition within distinct regions: the BA presented consistent amounts of new bone formation (NB), which increased over time (24.53%±1.26% at 1 month; 37.73%±0.39% at 3 months), thus suggesting that this area makes a substantial contribution toward NB. The PA was mainly composed of fibrous tissue (71.16%±8.06% and 78.30%±2.67%, respectively), while the CA showed high amounts of DBBM at both time points (78.30%±2.67% and 74.68%±1.07%, respectively), demonstrating a slow remodeling process. Blood vessels revealed a progressive migration from the interface with native bone toward the central area of the graft. Osterix-positive cells observed at 1 month within the PA suggested that the periosteum was a source of osteoprogenitor elements. Alkaline phosphatase data on matrix deposition confirmed this observation.ConclusionsThe present model allowed for a standardized investigation of distinct graft-to-host interfaces both at vertically augmented and inlay-augmented sites, thus possibly limiting the number of animals required for pre-clinical investigations.

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Canonical Wnt signaling differently modulates osteogenic differentiation of mesenchymal stem cells derived from bone marrow and from periodontal ligament under inflammatory conditions

Cited by 96 publications

References 32 publications

Periodontal Ligament Stem Cells in the Periodontitis Microenvironment Are Sensitive to Static Mechanical Strain

Periodontal Ligament Stem Cells in the Periodontitis Microenvironment Are Sensitive to Static Mechanical Strain

Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

“Over-inlay” block graft and differential morphometry: a novel block graft model to study bone regeneration and host-to-graft interfaces in rats

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