Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages

Nagendran, Monica; Arora, Prateek; Gori, Payal; Mulay, Aditya; Ray, Shinjini; Jacob, Tressa; Sonawane, Mahendra

doi:10.1242/dev.118703

Cited by 13 publications

(8 citation statements)

References 61 publications

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“…Furthermore, the outgrowth of the zebrafish’s ( Danio rerio ) median and pectoral fins is achieved by changes of epithelial cell shapes from elongated to round [ 50 ]. Similar cellular dynamics were even observed during the formation and elongation of vertebrate epithelial appendages, such as feathers, scales, hair, claws, and teeth [ 50 – 53 ]. Recent studies on the embryonic formation of octopus appendages have shown that actin-mediated epithelial cell shape changes (i.e., cell elongation and their alignment along the PD axis) are also crucial for the elongation of appendages in a cephalopod [ 22 ].…”

Section: Discussionmentioning

confidence: 59%

The cephalopod arm crown: appendage formation and differentiation in the Hawaiian bobtail squid Euprymna scolopes

et al. 2016

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BackgroundCephalopods are a highly derived class of molluscs that adapted their body plan to a more active and predatory lifestyle. One intriguing adaptation is the modification of the ventral foot to form a bilaterally symmetric arm crown, which constitutes a true morphological novelty in evolution. In addition, this structure shows many diversifications within the class of cephalopods and therefore offers an interesting opportunity to study the molecular underpinnings of the emergence of phenotypic novelties and their diversification. Here we use the sepiolid Euprymna scolopes as a model to study the formation and differentiation of the decabrachian arm crown, which consists of four pairs of sessile arms and one pair of retractile tentacles. We provide a detailed description of arm crown formation in order to understand the basic morphology and the developmental dynamics of this structure.ResultsWe show that the morphological formation of the cephalopod appendages occurs during distinct phases, including outgrowth, elongation, and tissue differentiation. Early outgrowth is characterized by uniform cell proliferation, while the elongation of the appendages initiates tissue differentiation. The latter progresses in a gradient from proximal to distal, whereas cell proliferation becomes restricted to the distal-most end of the arm. Differences in the formation of arms and tentacles exist, with the tentacles showing an expedite growth rate and higher complexity at younger stages.ConclusionThe early outgrowth and differentiation of the E. scolopes arm crown shows similarities to the related, yet derived cephalopod Octopus vulgaris. Parallels in the growth and differentiation of appendages seem to exist throughout the animal kingdom, raising the question of whether these similarities reflect a recruitment of similar molecular patterning pathways.Electronic supplementary materialThe online version of this article (doi:10.1186/s12983-016-0175-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 59%

The cephalopod arm crown: appendage formation and differentiation in the Hawaiian bobtail squid Euprymna scolopes

et al. 2016

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“…In human hepatocellular carcinoma (HCC), SP1 transactivates the LAMC1 promoter 18. Another study indicated that in HCC, LAMC1 expression is modulated by the Wnt signaling pathway 19. Ma NKL et al observed a collaboration between laminins and 3D contexts promoting astrocytoma stemness.…”

Section: Discussionmentioning

confidence: 99%

<p>High LAMC1 expression in glioma is associated with poor prognosis</p>

Liu

Yang

2019

OTT

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Purpose: Glioma is the most common malignant brain tumor. The molecular mechanisms underlying its malignancy are not fully understood. LAMC1, which encodes extracellular matrix protein laminin γ1, has been implicated in some malignant tumors but has not been systematically evaluated in glioma. The aim of this study was to evaluate the expression of LAMC1 and its clinical significance. Patients and methods: LAMC1 protein expression in 52 fresh-frozen specimens of different pathological grade gliomas and 5 normal brain tissues was detected by Western blotting. Immunohistochemistry was used to detect LAMC1 protein expression in another set of 76 glioma tissues and 8 normal brain tissues. The associations between clinicopathological factors and LAMC1 expression were analyzed. A log-rank test and a multivariate Cox proportional hazards model were used to determine the relationship between LAMC1 expression and patient prognosis. The expression of LAMC1 at the mRNA level was analyzed in the TCGA database. Results: LAMC1 was highly expressed in high-grade glioma tissues, with moderate expression in low-grade gliomas, and weak or no expression in normal brain tissues, as detected by Western blotting and immunohistochemistry. A chi-square test indicated that LAMC1 expression was associated with pathological grade but not with other clinicopathological factors, such as age, sex, and tumor size. LAMC1 expression at the mRNA level was upregulated in high-grade gliomas compared with low-grade gliomas and normal brain tissue in the TCGA database. The Kaplan–Meier plot and log-rank test in our patient series showed that high LAMC1 expression was significantly associated with shorter survival, which was consistent with the TCGA database analysis. A multivariate Cox proportional hazards model revealed that LAMC1 expression, WHO grade, and surgery procedure were significantly correlated with overall survival and progression-free survival. Conclusion: These results demonstrated that LAMC1 may play an important role in glioma progression and may be used in the diagnosis, prognosis, and targeted therapy of glioma patients.

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“…While our data implicate fgf20a as an inducer of lamb1a during fin regeneration, there may exist other regulatory inputs for this and distinct ECM factors. For example, Nagendran and colleagues recently reported in a morpholino-based study that lama5 expression is under control of canonical Wnt signaling in the embryonic zebrafish fin epithelium [ 56 ]. Thus, it is possible that the regeneration epidermis is shaped by a network of growth factor-ECM regulatory interactions.…”

Section: Discussionmentioning

confidence: 99%

Transient laminin beta 1a Induction Defines the Wound Epidermis during Zebrafish Fin Regeneration

et al. 2015

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The first critical stage in salamander or teleost appendage regeneration is creation of a specialized epidermis that instructs growth from underlying stump tissue. Here, we performed a forward genetic screen for mutations that impair this process in amputated zebrafish fins. Positional cloning and complementation assays identified a temperature-sensitive allele of the ECM component laminin beta 1a (lamb1a) that blocks fin regeneration. lamb1a, but not its paralog lamb1b, is sharply induced in a subset of epithelial cells after fin amputation, where it is required to establish and maintain a polarized basal epithelial cell layer. These events facilitate expression of the morphogenetic factors shha and lef1, basolateral positioning of phosphorylated Igf1r, patterning of new osteoblasts, and regeneration of bone. By contrast, lamb1a function is dispensable for juvenile body growth, homeostatic adult tissue maintenance, repair of split fins, or renewal of genetically ablated osteoblasts. fgf20a mutations or transgenic Fgf receptor inhibition disrupt lamb1a expression, linking a central growth factor to epithelial maturation during regeneration. Our findings reveal transient induction of lamb1a in epithelial cells as a key, growth factor-guided step in formation of a signaling-competent regeneration epidermis.

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Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages

Cited by 13 publications

References 61 publications

The cephalopod arm crown: appendage formation and differentiation in the Hawaiian bobtail squid Euprymna scolopes

The cephalopod arm crown: appendage formation and differentiation in the Hawaiian bobtail squid Euprymna scolopes

<p>High LAMC1 expression in glioma is associated with poor prognosis</p>

Transient laminin beta 1a Induction Defines the Wound Epidermis during Zebrafish Fin Regeneration

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