Canova, a Brazilian medical formulation, alters oxidative metabolism of mice macrophages

Oliveira, Carolina Camargo de; Oliveira, Simone Martins de; Godoy, Lyris Martins Franco de; Gabardo, Juarez; Buchi, Dorly de Freitas

doi:10.1016/j.jinf.2005.08.017

Cited by 40 publications

(36 citation statements)

References 45 publications

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“…On the other hand, they play an indirect role in these anti-microbial and anti-tumor activities by secretion of cytokines or by antigen processing and presentation, thereby regulating the immune system [Klimp et al, 2002]. We already found that CA treatment alters some of these Mf properties [Piemonte and Buchi, 2002;de Oliveira et al, 2006;Lopes et al, 2006]. We have now demonstrated that in vivo treatment with CA decreases the production of IL-2 and IL-4.…”

Section: Discussionmentioning

confidence: 71%

Gene expression profiling of macrophages following mice treatment with an immunomodulator medication

Oliveira

Góes

et al. 2008

J of Cellular Biochemistry

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Canova (CA) is a complex homeopathic medication used in diseases where the immune system is depressed. Previous studies demonstrated that it is neither toxic nor mutagenic and activates macrophages. We now evaluate CA effects on cytokine production and gene expression from mice macrophages. The global view of changes in expression of genes with known functions can provide a vivid picture of the way in which cell adapts to a changing environment or a challenge. We found a decrease in IL-2 and IL-4 production and a differential expression in 147 genes from CA group. These genes are mainly involved in transcription/translation, cell structure and dynamics, immune response, cytoprotection, enzymatic process, and receptors/ligands. With gene expression analysis we state that this medication provokes a reaction that involves alterations in gene expression profile mainly in the ones involved with macrophages activation, corroborating the laboratorial research and the clinical data.

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Section: Discussionmentioning

confidence: 71%

Gene expression profiling of macrophages following mice treatment with an immunomodulator medication

Oliveira

Góes

et al. 2008

J of Cellular Biochemistry

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“…Our group also previously observed that CA induces macrophage activation and indirectly leads to lymphocyte proliferation in vitro [1] . CA activated macrophages present an increase in the intracellular activity of the NAD(P)H oxidase and Nitric Oxide Sintetase (NOS) enzymes, therefore stimulating the production of reactive oxygen species (ROS) and nitric oxide, which might induce gene expression and/or protein modification that are likely to be an alternative signaling pathway to activate other macrophages, favoring the immune response [9] .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Biochemical, Hematological and Genotoxic Parameters of Cebus Apella Treated with N-Methyl-Nitrosurea (NMU) Followed by Treatment with a Complex Homeopathic Compound (Canova)

Burbano¹

2016

JBT

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Canova is a homeopathic compound that presents antimutagenic and anticancer properties. N-methylnitrosourea is a carcinogenic agent that causes alterations on DNA. This study was conducted to evaluate the antimutagenic effects of Canova on Cebus apella non-human primates treated with N-methyl-nitrosourea. Six animals were randomly assigned in two groups, one composed by two animals, who received only N-methyl-nitrosourea (NMU group) and another one consisting on four animals treated with N-methyl-nitrosourea followed by Canova treatment (CA group). Body weight, biochemical and hematological analysis were performed. Micronuclei test and Comet assay were carried out to evaluate genotoxicity. N-methyl-nitrosourea treatment led to an overall reduction in the hematologic cell count and the administration of Canova limited the adverse effects in the hematologic system on CA group. Canova also reduced micronuclei frequency and the DNA damage index. It suggests that Canova may have a role as an immune response modulator by inducing leukocyte proliferation.

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“…An initial dose of 20% of M8 treatment was administered to the cells and, after 24 h, a reinforcement dose of 1% was administered according to a previous standard treatment protocol [10,16]. …”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo anticancer properties of a Calcarea carbonica derivative complex (M8) treatment in a murine melanoma model

et al. 2010

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BackgroundMelanoma is the most aggressive form of skin cancer and the most rapidly expanding cancer in terms of worldwide incidence. Chemotherapeutic approaches to treat melanoma have had only marginal success. Previous studies in mice demonstrated that a high diluted complex derived from Calcarea carbonica (M8) stimulated the tumoricidal response of activated lymphocytes against B16F10 melanoma cells in vitro.MethodsHere we describe the in vitro inhibition of invasion and the in vivo anti-metastatic potential after M8 treatment by inhalation in the B16F10 lung metastasis model.ResultsWe found that M8 has at least two functions, acting as both an inhibitor of cancer cell adhesion and invasion and as a perlecan expression antagonist, which are strongly correlated with several metastatic, angiogenic and invasive factors in melanoma tumors.ConclusionThe findings suggest that this medication is a promising non-toxic therapy candidate by improving the immune response against tumor cells or even induce direct dormancy in malignancies.

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Canova, a Brazilian medical formulation, alters oxidative metabolism of mice macrophages

Cited by 40 publications

References 45 publications

Gene expression profiling of macrophages following mice treatment with an immunomodulator medication

Gene expression profiling of macrophages following mice treatment with an immunomodulator medication

Evaluation of the Biochemical, Hematological and Genotoxic Parameters of Cebus Apella Treated with N-Methyl-Nitrosurea (NMU) Followed by Treatment with a Complex Homeopathic Compound (Canova)

In vitro and in vivo anticancer properties of a Calcarea carbonica derivative complex (M8) treatment in a murine melanoma model

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