In vitro and in vivo anticancer properties of a Calcarea carbonica derivative complex (M8) treatment in a murine melanoma model

Abstract

BackgroundMelanoma is the most aggressive form of skin cancer and the most rapidly expanding cancer in terms of worldwide incidence. Chemotherapeutic approaches to treat melanoma have had only marginal success. Previous studies in mice demonstrated that a high diluted complex derived from Calcarea carbonica (M8) stimulated the tumoricidal response of activated lymphocytes against B16F10 melanoma cells in vitro.MethodsHere we describe the in vitro inhibition of invasion and the in vivo anti-metastatic potential after M8 treatment by inhalation in the B16F10 lung metastasis model.ResultsWe found that M8 has at least two functions, acting as both an inhibitor of cancer cell adhesion and invasion and as a perlecan expression antagonist, which are strongly correlated with several metastatic, angiogenic and invasive factors in melanoma tumors.ConclusionThe findings suggest that this medication is a promising non-toxic therapy candidate by improving the immune response against tumor cells or even induce direct dormancy in malignancies.

Anti-proliferative effects of homeopathic medicines on human kidney, colon and breast cancer cells

“…A recent study showing in vitro and in vivo anticancer properties of Calcarea carbonica derivative complex M8 (highly diluted natural product) in a murine melanoma model, demonstrated inhibition of tumor growth and metastasis by decrease in perlecan expression (component of basement matrix and its expression correlates strongly with the expression of several metastatic, angiogenic and invasive factors in tumor cells, particularly melanoma cells) and a direct inhibition of cancer cell adhesion and invasion. 22,23 Another study revealed that on treatment with a complex homeopathic medication (Calcarea carbonica CH5) there was an indirect activation of lymphocytes through their interaction with macrophages, even without direct cellecell contact. The co-culture of macrophages and lymphocytes in the presence of CH5 promoted immunostimulation of lymphocytes, resulting in enhanced tumoricidal performance against a very aggressive line of melanoma cells.…”

“…Guimarães et al attributed the anti tumoricidal effect of homeopathic preparations on their ability to prime the immune system to seek out and destroy cancer cells. 23 It is possible that in vivo ultra-diluted higher potencies alter the immune system resulting in enhanced cytotoxicity against cancer cells. But our in vitro study was based on homogenous cell populations, with no immune cell component.…”

Inhalation therapy with M1 inhibits experimental melanoma development and metastases in mice

“…14 This pre-clinical study presented here provides data supporting the efficacy of M1 for melanoma therapy. Using a method based on combinatory and high occurrence rates and low doses of M1 inhaled, as previously described, 15 we show for the first time that M1 treatment reduced the tumor burden of an experimental pulmonary metastasis model and primary tumor formation of mouse melanoma. In summary, we identified physiologic factors leading to the control of initial pathways of tumor metastasis and growth.…”

“…Solutions were vigorously homogenized and immediate applied to animals via oral inhalation as previously described. 15 Briefly, animals were placed into oral inhalation chambers and treated for 10 min, twice a day, 12 h apart for a period of 14 days. Following 15 days of cell inoculation, mice were anesthetized with 100 mg/kg ketamine (DopaleneVetbrands) and 20 mg/kg xilazin (AnasedaneVetbrands) followed by euthanasia by cervical dislocation.…”

Homeopathy: Curative, Concurrent and Supportive Cancer Treatment Potential

“…In vivo C. carbonica (M8) diluent directly inhibits B16F10 murine melanoma cell adhesion and invasion, and inhibits melanoma growth and metastasis by decreasing perlecan (heparin sulfate proteoglycan 2 [HSPG2]) expression [4,20]. C. carbonica 6C produces 30% to 35% cancer cell apoptosis in Ehrlich's ascites carcinoma (EAC), Sarcoma-180 (S-180), MCF-7, MDA-MB-231, and HBL-100 breast cancer bearing Swiss albino mice, providing a survival advantage, p<0.001, by Student's t test [21].…”