Capability of human mesenchymal cells isolated from different sources to differentiation into tissues of mesodermal origin

Suzdaltseva, Yu. G.; Burunova, V. V.; Вахрушев, И. В.; Yarygin, V. N.; Yarygin, K. N.

doi:10.1007/s10517-007-0030-1

Cited by 28 publications

(21 citation statements)

References 4 publications

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“…Whereas some investigators describe an osteogenic potential comparable to bone marrow MSC [55,56] and even higher [32], others show that cells collected from human UC are poorly osteogenic and only some of them are capable to undergo osteogenic differentiation [57,58]. Our data corroborate the last thesis.…”

Section: Discussionsupporting

confidence: 87%

“…It is, however, conceivable that the differentiation potential of UC-derived cells strongly depends on their location within the UC tissue. Thus, Suzdal'tseva et al [57] and Girdlstone et al [58] reported that only a moderate number of cells isolated from vascular and Wharton's jelly fractions of UC was able to differentiate into osteoblasts. In contrast, cells harvested from perivascular regions showed even higher osteogenic capacity than bone marrow MSC in a comparative study [32].…”

Section: Discussionmentioning

confidence: 99%

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Growth and Differentiation Properties of Mesenchymal Stromal Cell Populations Derived from Whole Human Umbilical Cord

Majore

Moretti

Stahl

et al. 2010

Stem Cell Rev and Rep

149

106

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Up to 2.8 × 10(7) fibroblast-like cells displaying an abundant presence of mesenchymal stem cell (MSC) markers CD73, CD90, CD105 and a low level of HLA-I expression can be isolated from one whole human umbilical cord (UC) using a simple and highly reproducible explant culture approach. Cells derived from whole UC, similar to cells collected from separate compartments of UC, display a distinct chondrogenic and adipogenic potential. Therefore they are potential candidates for cartilage and adipose tissue engineering. Cell differentiation along the osteogenic pathway is, however, less efficient, even after the addition of 1.25-dihydroxyvitamin D3, a potent osteoinductive substance. Isolated cells are highly proliferative, tolerate cryopreservation with an average survival rate of about 75% and after thawing can be propagated further, at least over 20 population doublings before their proliferative activity begins to decline. More importantly, they synthesize numerous trophic factors including neurotrophins and factors which facilitate angiogenesis and hematopoiesis. In conclusion, cells isolated from whole UC satisfies all requirements essential for the generation of stem cell banks containing permanently available cell material for applications in the field of regenerative medicine. Nevertheless, further studies are needed to improve and adjust the methods which are already employed for adult MSC expansion and differentiation to specific properties and requirements of the primitive stem cells collected from UC. So, our data verify that the choice of individual parameters for cell propagation, such as duration of cell expansion and cell seeding density, has a substantial impact on the quality of UC-derived cell populations.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Growth and Differentiation Properties of Mesenchymal Stromal Cell Populations Derived from Whole Human Umbilical Cord

Majore

Moretti

Stahl

et al. 2010

Stem Cell Rev and Rep

149

106

View full text Add to dashboard Cite

show abstract

“…Interestingly, the only major difference is their differentiation potential [6]. UC-MSCs have a very weak osteogenic [18][19][20], chondrogenic [21], and adipogenic [19,20] differentiation capacity compared with BM-MSCs. The reasons for these differences need to be further clarified, but it has been postulated that the reduced differentiation potential of UC-MSCs may depend on their position within the UC tissue they are isolated from [22].…”

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confidence: 99%

Transplanted Umbilical Cord Mesenchymal Stem Cells Modify the In Vivo Microenvironment Enhancing Angiogenesis and Leading to Bone Regeneration

Todeschi

Backly

Capelli

et al. 2015

Stem Cells and Development

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Umbilical cord mesenchymal stem cells (UC-MSCs) show properties similar to bone marrow mesenchymal stem cells (BM-MSCs), although controversial data exist regarding their osteogenic potential. We prepared clinical-grade UC-MSCs from Wharton's Jelly and we investigated if UC-MSCs could be used as substitutes for BM-MSCs in muscoloskeletal regeneration as a more readily available and functional source of MSCs. UCMSCs were loaded onto scaffolds and implanted subcutaneously (ectopically) and in critical-sized calvarial defects (orthotopically) in mice. For live cell-tracking experiments, UC-MSCs were first transduced with the luciferase gene. Angiogenic properties of UC-MSCs were tested using the mouse metatarsal angiogenesis assay. Cell secretomes were screened for the presence of various cytokines using an array assay. Analysis of implanted scaffolds showed that UC-MSCs, contrary to BM-MSCs, remained detectable in the implants for 3 weeks at most and did not induce bone formation in an ectopic location. Instead, they induced a significant increase of blood vessel ingrowth. In agreement with these observations, UC-MSC-conditioned medium presented a distinct and stronger proinflammatory/chemotactic cytokine profile than BM-MSCs and a significantly enhanced angiogenic activity. When UC-MSCs were orthotopically transplanted in a calvarial defect, they promoted increased bone formation as well as BM-MSCs. However, at variance with BM-MSCs, the new bone was deposited through the activity of stimulated host cells, highlighting the importance of the microenvironment on determining cell commitment and response. Therefore, we propose, as therapy for bone lesions, the use of allogeneic UC-MSCs by not depositing bone matrix directly, but acting through the activation of endogenous repair mechanisms.

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“…To shed some light on the biology of MSC, consensus conferences coined some criteria to better define MSC from bone marrow (bmMSC) [27,28] or placenta (pMSC) [29,30]. But an in-depth methodical examination to define MSC from adipose (aMSC) [31] or other tissues was not presented [8,25,[32][33][34]. Although the differences between MSC from different sources constitute an interesting field for research and reflection, in this review we discuss expression of cell surface markers to discriminate MSC from other cells and address the problems and recent studies of multilineage differentiation of both MSC and dermal fibroblasts (DF).…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stromal Cells and Fibroblasts

Ulrich¹

2012

J Tissue Sci Eng

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SummaryAdult mesenchymal stromal cells (MSC), also referred to as mesenchymal stem cells, were detected almost half a century ago in bone marrow and have been studied intensively in the last decade. Different aspects of MSC biology were explored and published. Studies pointed to their localization in different organs during development and in adulthood and described their characteristics in experimental or clinical investigations. Despite intensive research in the field and in sharp contrast to hematopoietic stem cells (HSC), it has become more and more clear that MSC lack a unique cell surface marker. MSC not only share cell surface markers with other types of cells, they also share many features with pericytes and fibroblasts, including their capability to differentiate into, for instance, osteoblasts or adipocytes. In this review we therefore screen the current literature to disclose differences between MSC and fibroblasts and also report on common qualities.

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Capability of human mesenchymal cells isolated from different sources to differentiation into tissues of mesodermal origin

Cited by 28 publications

References 4 publications

Growth and Differentiation Properties of Mesenchymal Stromal Cell Populations Derived from Whole Human Umbilical Cord

Growth and Differentiation Properties of Mesenchymal Stromal Cell Populations Derived from Whole Human Umbilical Cord

Transplanted Umbilical Cord Mesenchymal Stem Cells Modify the In Vivo Microenvironment Enhancing Angiogenesis and Leading to Bone Regeneration

Mesenchymal Stromal Cells and Fibroblasts

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