Capacitation of Rabbit Spermatozoa in vitro

Brackett, Benjamin G.; Oliphant, Gene

doi:10.1095/biolreprod12.2.260

Cited by 1,048 publications

(469 citation statements)

References 47 publications

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“…Brackett & Oliphant's (BO) medium,17 containing 1% bovine serum albumin (Wako, Tokyo, Japan) was used for the washing and dilution of the sperm and for the column construction. The sperm capacitation‐inducing medium, or HC, contained both 15 μg/mL of heparin (Mochida, Tokyo, Japan) and 5 mmol/L of caffeine (SIGMA‐Aldrich, St. Louis, MO, USA) with the BO medium.…”

Section: Methodsmentioning

confidence: 99%

Possible ability of bovine follicular fluid to attract migrating bull spermatozoa

Mondal

Takagi

Baba

et al. 2017

Reprod Medicine & Biology

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AimTo examine the potential of bovine follicular fluid (BFF) to attract bull spermatozoa.MethodsThe ability of the BFF to attract bull sperm was evaluated by observing changes in sperm migration after being placed in a cross‐column chamber. The movement parameters of the heads and flagella of the sperm that were attracted to the BFF were analyzed by using the Computer Assisted Sperm Analysis system.ResultsIt was observed that 61.6% of the bull sperm migrated toward the BFF when the BFF was used at a concentration of 0.1%, but 67.2% of the sperm did not migrate toward the BFF at a concentration of 10%. Relatively larger numbers of both precapacitated and postcapacitated bull sperm migrated toward the BFF (0.1%). The ability of the 0.1% BFF to attract sperm probably affected both the normal artificial insemination (AI) fertility sperm and the poor AI fertility spermatozoa. The flagellar curvilinear ratio of the sperm winding to the 0.1% BFF was significantly higher than that of the prewinding sperm.ConclusionThese results could suggest that BFF potentially attracts bull sperm at a certain concentration, irrespective of the capacitation status of the sperm. Although the mechanism by which this attraction occurs remains unclear, these data imply that it could be related to BFF‐dependent changes in the sperm flagellar curvilinear ratio.

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Section: Methodsmentioning

confidence: 99%

Possible ability of bovine follicular fluid to attract migrating bull spermatozoa

Mondal

Takagi

Baba

et al. 2017

Reprod Medicine & Biology

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“…Two 0.5 ml straws thawed for 60 s at 358C were emptied into 15 ml air-tight tube and washed with Brackett and Oliphant (BO) medium containing heparin 10 mg/ml (Brackett and Oliphant, 1975) without BSA by centrifugation at 700 3 g for 5 min. The sperm pellet was mixed with 2 ml of BSA-free BO medium containing 10 mM of caffeine (sodium caffeine benzoate) and 10 mg/ml heparin in air tight tube and placed into the incubator at 38.58C for swim-up for 1 h, after which the top 1.8 ml of the semen suspension containing motile spermatozoa were removed through micropipettes and washed twice with BSA-free BO medium.…”

Section: Methodsmentioning

confidence: 99%

Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos

Thiyagarajan

Valivittan

2009

Animal

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Vitamin A is a well-known antioxidant and is essential for embryonic development, growth and differentiation. Oxidative stress is involved in the etiology of defective embryo development. The present study evaluated whether the presence of all-trans retinol (0, 1, 2, 5 and 10 mM) in maturation medium or embryo culture medium would enhance the developmental competence of preimplantation buffalo embryos in vitro. In experiment I, cumulus oocytes complex were matured with varying concentrations of all-trans retinol. Treatment with 5 mM all-trans retinol improved the blastocyst formation ( P , 0.001) when compared with control and significant increase ( P , 0.01) in total cell number was observed in 5 mM group when compared with control. Supplementation of all-trans retinol in embryo culture medium for the entire culture period under 5% O 2 and 20% O 2 was tested in experiments II and III, respectively. Supplementation of 10 mM all-trans retinol under 5% O 2 , significantly reduced blastocyst formation and cell numbers. Presence of 5 mM all-trans retinol under 20% O 2 enhanced the frequency of blastocyst formation and total cell number ( P , 0.001) when compared with control. DNA damage of individual embryos cultured under 20% oxygen concentration was measured by the comet assay. Supplementation of 5 mM all-trans retinol significantly reduced the comet tail ( P , 0.001) when compared with control. Supplementation of all-trans retinol in embryo culture medium for first 72 h of the 8-day culture period under 5% O 2 was tested in experiment IV. Addition of 5 mM all-trans retinol resulted in significant increase in blastocyst rate and total cell number ( P , 0.001) when compared with control. Our results demonstrate that addition of all-trans retinol to maturation or embryo culture medium may enhance the developmental competence of buffalo embryos in vitro by enhancing blastocyst formation rate and total cell number.

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“…Spermatozoa were capacitated using Brackett and Oliphant medium (Bracket and Oliphant 1975). Frozen-thawed semen from the same bull was used in all experiments.…”

Section: Sperm Capacitationmentioning

confidence: 99%

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

et al. 2016

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The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented with 10 % FBS and defined PVA-myo-inositol-phosphate-EGF medium. No (P [ 0.05) differences at 2-cell, 4-cell and 8-cell to 16-cell stages were observed among the three cultured media used, however, increased (P \ 0.05) blastocyst yield, cell number and hatching rate were found in defined medium compared to undefined media. The expression patterns of genes implicated in embryo metabolism (GLUT-1), anti-apoptosis (BCL-2), imprinting (IGF-2R), DNA methylation (DNMT-3A) and maternal recognition of pregnancy (IFNT) were increased (P \ 0.05) in hatched blastocysts derived from defined medium compared to undefined media. In conclusion, serum-free, defined medium improved developmental competence of in vitro cultured buffalo embryos. Whether these differences in morphological development and gene expression have long-term effects on buffalo calves born after embryo transfer remains unknown. However, it is possible that early adaptations of the preimplantation embryo to its environment persist during fetal and post-natal development.

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Capacitation of Rabbit Spermatozoa in vitro

Cited by 1,048 publications

References 47 publications

Possible ability of bovine follicular fluid to attract migrating bull spermatozoa

Possible ability of bovine follicular fluid to attract migrating bull spermatozoa

Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

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