Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos

Thiyagarajan, B.; Valivittan, K.

doi:10.1017/s1751731108003455

Cited by 4 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Akçay et al [1] reported similar findings about the maturation and fertilization of bovine oocytes under the effects of serum, steroid and gonadotropins. Similar findings about the effective activities of cis-retinal acids on proportion of oocytes that reached the metaphase II stage and also in vitro embryo production were reported from Spain [20] , India [21] , Iran [22] and United States [23] . Öztürkler et al [2] reported that the percentages of oocyte maturation, cleavage, 8-cell and morula stage embryos in L-ergothioneine supplemented group were significantly higher compared with the other groups.…”

Section: Discussionsupporting

confidence: 85%

Sığır İn Vitro Embriyo Üretiminde Sistamin ve 13-Cis-Retinoik Asitin Etkisi

Ranjbar¹,

Eslampour²,

Moghadam³

2018

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

One of the main factors decreasing the success of an in vitro embryo production is oxidative stress-mediated reactive oxygen species resulting in cellular damages. The present study was carried out to evaluate the effects of addition of different doses of cysteamine and 13-cis-retinoic acid during in vitro maturation on oocyte maturation and blastocyst formation. Five-hundred oocyte complexes were randomly collected from slaughterhouse and assigned to five groups; oocytes matured in vitro maturation (IVM) medium without any supplementation (control), IVM medium supplemented with 50 µM or 100 µM cysteamine, IVM supplemented with 5 nM 13-cis-retinoic acid and IVM supplemented with 10 nM 13-cis-retinoic acid. Matured oocytes were fertilized and cultured. Oocytes and embryos were evaluated for nuclear maturationand blastocyst formation, respectively. The highest numbers of oocytes in the cumulus expansion, cleavage, murola and blastocyst stages were seen in cows treated with 13-cis retinoic (5 nm) (92%), cysteamine (100 µL) (74%), cysteamine (50 and 100 µL) (48%), cysteamine (100 µL) and 13-cis retinoic (24%) and finally cysteamine (100 µL) (22%), respectively.Nuclear maturation of oocytes decreased significantly in the media supplemented with 10 nM 13-cis-retinoic acid (P<0.05). The results of the present study demonstrated that IVM supplementation with cysteamine and a low concentration of 13-cis-retinoic acid improved the efficacy of an in vitro production of bovine embryo.

show abstract

Section: Discussionsupporting

confidence: 85%

Sığır İn Vitro Embriyo Üretiminde Sistamin ve 13-Cis-Retinoik Asitin Etkisi

Ranjbar¹,

Eslampour²,

Moghadam³

2018

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

show abstract

“…As shown in Table 5, t-RA has no effect on goat parthenogenetic cleavage, parthenogenetic blastocysts and total cell numbers. The formation of blastocysts and total cell numbers of buffalo (Thiyagarajan & Valivittan 2009) and bovine (Liu et al 2004) were significantly stimulated by 5 μmol/L (all-trans) retinol treated during maturation of COCs. But all-trans 65.1 ± 0.9 73.3 ± 1.4 N = number; values were the mean ± SEM; within a column, the experimental groups were compared with the control group, **Meant statistically highly significant difference (P < 0.01), *Meant statistically significant difference (P < 0.05).…”

Section: Discussionmentioning

confidence: 99%

“…As shown in Table , t ‐RA has no effect on goat parthenogenetic cleavage, parthenogenetic blastocysts and total cell numbers. The formation of blastocysts and total cell numbers of buffalo (Thiyagarajan & Valivittan ) and bovine (Liu et al . ) were significantly stimulated by 5 μmol/L ( all‐trans ) retinol treated during maturation of COCs.…”

Section: Discussionmentioning

confidence: 99%

All‐trans retinoic acid improves goat oocyte nuclear maturation and reduces apoptotic cumulus cells during in vitro maturation

Wang

Bian

et al. 2014

Animal Science Journal

View full text Add to dashboard Cite

All-trans retinoic acid (t-RA) is a natural component and representative physiologically active metabolite of vitamin A, having multiple physiologic functions. The objective of this study was to evaluate the effect of t-RA on goat oocyte maturation and cumulus cell apoptosis during in vitro maturation (IVM). Immature goat cumulus-oocyte complexes (COCs) were matured in vitro in the absence or presence of t-RA at concentrations of 10 nmol/L, 100 nmol/L and 1000 nmol/L. Oocyte maturation and embryo development were assessed by polar body formation and parthenogenetic activation, respectively. Oocyte survival was checked by Trypan blue staining. Apoptosis of cumulus cells was analyzed by terminal deoxynucleotidyl transferase nick end labeling staining and quantitative real-time PCR. In comparison with the control group, 100 nmol/L and 10 nmol/L t-RA significantly improved goat nuclear oocyte maturation and survival (P < 0.05). Addition of 1000 nmol/L t-RA improved nuclear maturation (P < 0.05), but had no effect on survival of goat oocytes. t-RA had no positive effect on goat parthenogenetic embryonic cleavage, blastocyst formation or total cell numbers. However, t-RA inhibits the apoptosis of cumulus cells (P < 0.01). t-RA treatment up-regulated the expression of B-cell lymphoma 2 (BCL-2), catalase (CAT) (P < 0.05) and down-regulated the expression of Caspase-8 (P < 0.05). In conclusion, t-RA has positive effects on goat oocyte nuclear maturation and reduces apoptotic cumulus cells during IVM.

show abstract

“…Sel diinkubasi selama 24 jam, 9 kemudian diberi retinol pada media perbenihan adiposit dengan kepekatan retinol 0,1 μM, 1 μM, 10 μM. 9 Sel adiposit jumlah sekitar 5,0x10 2 12 yang menyebutkan bahwa adiposit matur dari jaringan adiposis memiliki bentuk sel bulat dengan tetesan lemak yang sangat besar, sehingga menempatkan inti sel (nukleus) dan sitoplasma di bagian tepi sel.…”

Section: -203unclassified

Phosphatidylinositol -3kinase (Pi3k) Di Perbenihan Adiposit Yang Dipajan Glukosa Tinggi Dengan Retinol

Firani¹,

Prijadi²

2018

Indonesian J. Clin. Pathol. Med. Lab.

View full text Add to dashboard Cite

Retinol is one of the active forms of vitamin A. In the previous study, it was known that retinol level in serum of DM patient waslower than in healthy people, which correlated with an increase of the glucose levels in these patients. The importance of retinol in insulinsignaling mechanisms that play a role in the pathogenesis of DM is still unknown. One of the components that play a role in insulinsignaling on adipocytes is phosphatidylinositol-3 kinase (PI3K), which encourages the translocation of glucose transporter-4 (GLUT4) tothe cell surface. The aim of this study was to know the importance of retinol therapy in the levels of PI3K enzyme on visceral adipocyteculture with high glucose exposure (25 mM) as a model of DM in vitro by determination method. Retinol therapy was given at a doseof 0.1 μM, 1 μM , and 10 μM. Measurement of PI3K level was done by ELISA method. The mean (SD) levels of PI3K enzyme were 1.91(0.27), 0.94 (0.15), 1.98 (0.22), 1.69 (0.81), 2.04 (0.16) ng/mL respectively, for adipocyte cultures exposed to 5mM glucose (as aphysiological condition), 25mM glucose, and 25mM glucose concentration with doses of retinol therapy 0.1 μM, 1 μM and10 μM. Theresults of this study indicated that high glucose exposure (25 mM) decreased the level of PI3K compared with adipocyte’s culture on5 mM glucose exposure. Retinol therapy with a dose of 0.1μM, 1μM and10 μM on adipocyte culture exposed with high glucose couldincrease the levels of PI3K.

show abstract

Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos

Cited by 4 publications

References 42 publications

Sığır İn Vitro Embriyo Üretiminde Sistamin ve 13-Cis-Retinoik Asitin Etkisi

Sığır İn Vitro Embriyo Üretiminde Sistamin ve 13-Cis-Retinoik Asitin Etkisi

All‐trans retinoic acid improves goat oocyte nuclear maturation and reduces apoptotic cumulus cells during in vitro maturation

Phosphatidylinositol -3kinase (Pi3k) Di Perbenihan Adiposit Yang Dipajan Glukosa Tinggi Dengan Retinol

Contact Info

Product

Resources

About