Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum

Arya, Sunil K.; Zhurauski, Pavel; Jolly, Pawan; Batistuti, Marina R.; Mulato, Marcelo; Estrela, Pedro

doi:10.1016/j.bios.2017.11.013

Cited by 133 publications

(69 citation statements)

References 42 publications

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“…Physiological levels of HER2 in the blood of healthy people range from 4 to 14 ng mL −1 , while its concentration in the blood of cancer patients is higher (14–75 ng mL −1 ). HER2 is over‐expressed in around 20–30 % of breast cancer patients and is also associated with an aggressive form of the disease and a poor prognosis ,. Higher levels of HER2 were also observed during gastric, oesophageal, ovarian and endometrial cancers.…”

Section: Eis As a Powerful Tool For Sensitive Detection Of Cancer Biomentioning

confidence: 99%

“…A thiol‐terminated DNA aptamer with an affinity towards HER2 was used to prepare the bio‐recognition layer via self‐assembly on interdigitated gold surfaces . Non‐Faradaic EIS measurements were used as a detection scheme by detecting changes in capacitance.…”

Section: Eis As a Powerful Tool For Sensitive Detection Of Cancer Biomentioning

confidence: 99%

“…Non‐Faradaic EIS measurements were used as a detection scheme by detecting changes in capacitance. The aptasensor exhibited a linear range for the determination of HER2 from 1 pM to 100 nM in both a buffer and in undiluted serum with LOD below 1 pM …”

Section: Eis As a Powerful Tool For Sensitive Detection Of Cancer Biomentioning

confidence: 99%

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Electrochemical Impedance Spectroscopy Based Biosensors: Mechanistic Principles, Analytical Examples and Challenges towards Commercialization for Assays of Protein Cancer Biomarkers

et al. 2018

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affinity biosensors are, without any doubt, among the most sensitive analytical devices available, offering low limits of detection and wide linear response ranges. There are, however, only a few papers detailing the application of impedimetric biosensors for the analysis of clinically relevant samples with due clinical performance. The fact that these devices have not found their way to any commercial or clinical use to date might be surprising, since an electrochemical assay platform based on portable potentiostats is a success story for monitoring a range of clinical parameters such as ions, haematological indicators and glucose. This review discusses the reasons behind this discrepancy and addresses the barriers to be overcome in order to achieve the point-of-care diagnostics using such devices for detection of protein oncomarkers approved by FDA. The final part of the review covers the most recent progress in the area.[a] Dr.

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Section: Eis As a Powerful Tool For Sensitive Detection Of Cancer Biomentioning

confidence: 99%

Section: Eis As a Powerful Tool For Sensitive Detection Of Cancer Biomentioning

confidence: 99%

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Electrochemical Impedance Spectroscopy Based Biosensors: Mechanistic Principles, Analytical Examples and Challenges towards Commercialization for Assays of Protein Cancer Biomarkers

et al. 2018

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“…They were commonly used as the immobilization matrix in the fabrication of biosensors to increase the efficiency of biosensors, which promotes biosensor stability and full functionality. [26][27][28][29] Gold microstructures have lately attracted considerable attention due to their unique physical and chemical characteristics distinct from the bulk Au and broad range of uses in the optical, digital, catalytic, electrochemical, and biomedical industries. Several efficient pathways may be used for the preparing of flower-like, cube, sphere, plate, and rod gold micro-/ nanostructures.…”

Section: Introductionmentioning

confidence: 99%

<p>An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker</p>

Maghsoudi¹,

Hassani²,

Akmal³

et al. 2020

IJN

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In the present study, a highly sensitive and simple electrochemical (EC) aptasensor for the detection of serpin A12 as a novel biomarker of diabetes was developed on a platform where flower-like gold microstructures (FLGMs) are electrodeposited onto a disposable screen-printed carbon electrode. Meanwhile, serpin A12-specific thiolated aptamer was covalently immobilized on the FLGMs. Methods: The electrochemical activity of a fabricated aptasensor under various conditions were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Aptamer concentration, deposition time, self-assembly time, and incubation time were optimized for assay of serpin A12. The differential pulse voltammetry (DPV) was implemented for quantitative detection of serpin A12 in K 3 [Fe (CN) 6 ]/K 4 [Fe (CN) 6 ] solution (redox probe). Results: The label-free aptasensor revealed a linear range of serpin A12 concentration (0.039-10 ng/mL), detection limit of 0.020 ng/mL (S/N=3), and 0.031 ng/mL in solution buffer and plasma, respectively. Conclusion: The results indicate that this aptasensor has a high sensitivity, selectivity, stability, and acceptable reproducibility for detection of serpin A12 in diabetic patients.

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“…In the biosensing application, gold is a promising material and plays a tremendously role for the better and high-performance sensing by acting as electroactive intermediates 23 . This is because of the better conductivity and capable of providing a biocompatible surface for the immobilization of biomolecules in a better arrangement 24 . Additionally, this arrangement may increase the affinity of antigen-antibody binding 25,26 .…”

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confidence: 99%

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

Letchumanan

Arshad

Gopinath

et al. 2020

Sci Rep

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Field of generating a surface thin film is emerging broadly in sensing applications to obtain the quick and fast results by forming the high-performance sensors. Incorporation of thin film technologies in sensor development for the better sensing could be a promising way to attain the current requirements. This work predominantly delineates the fabrication of the dielectric sensor using two different sensing materials (Gold and Aluminium). conventional photolithography was carried out using silicon as a base material and the photo mask of the dielectric sensor was designed by AutocAD software. the physical characterization of the fabricated sensor was done by Scanning electron Microscope, Atomic Force Microscope, High Power Microscope and 3D-nano profiler. The electrical characterization was performed using Keithley 6487 picoammeter with a linear sweep voltage of 0 to 2 V at 0.01 V step voltage. By pH scouting, i-V measurements on the bare sensor were carried out, whereby the gold electrodes conducts a least current than aluminium dielectrodes. comparative analysis with pH scouting reveals that gold electrode is suitable under varied ionic strengths and background electrolytes, whereas aluminium electrodes were affected by the extreme acid (pH 1) and alkali (pH 12) solutions.

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Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum

Cited by 133 publications

References 42 publications

Electrochemical Impedance Spectroscopy Based Biosensors: Mechanistic Principles, Analytical Examples and Challenges towards Commercialization for Assays of Protein Cancer Biomarkers

Electrochemical Impedance Spectroscopy Based Biosensors: Mechanistic Principles, Analytical Examples and Challenges towards Commercialization for Assays of Protein Cancer Biomarkers

<p>An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker</p>

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

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