Capacitive immunosensor for the detection of host cell proteins

Microcontact-BSA imprinted capacitive biosensor for real-time, sensitive and selective detection of BSA

“…Proper insulation of the electrode surface is an important step in the capacitive biosensor assay [40], [41], [42], [43], [44], [45], [46], [47]. Cyclic voltammetry (CV) is the generally used method in the presence of a permeable redox couple to evaluate the degree of insulation of the electrode surface.…”

“…Capacitive biosensors are the electrochemical sensors that measure changes in the dielectric properties when an analyte interacts with a biorecognition element on the sensor surface, causing a decrease in the capacitance [36], [37], [38], [39], [40], [41]. Capacitive biosensors have been used for the detection of various analytes like antigens, antibodies, proteins and heavy metal ions [42], [43], [44], [45], [46], [47]. These types of biosensors have a lot of advantages like inherent rapidity, high sensitivity, simplicity, low cost, easy manipulation and real-time measurement without labeling.…”

Host cell protein analysis in therapeutic protein bioprocessing – methods and applications

“…Among these, ELISA, electrophoresis, and MS are the most common techniques and are sometimes used in parallel for detailed characterization [28]. A range of other methods, suitable for on‐ and offline detection have also been developed, but are not yet in widespread use [25, 29–32].…”

“…An overview of the different biosensor technologies can be found in Mattiasson et al [43]. Teeparuksapun et al [31] developed a capacitive immunosensor to measure HCP concentrations. A flow injection method using sputtered gold electrodes was used.…”

Affinity sensor based on immobilized molecular imprinted synthetic recognition elements

“…Disposable electrodes were prepared by use of thermal evaporation to coat a silicon wafer with chromium (50 nm) and gold (200 nm) as described by Teeparuksapun et al (2009Teeparuksapun et al ( , 2012. Before coupling, the electrode surface was cleaned to remove protective coatings on the surface.…”