Capacitive sensing of microcystin variants of Microcystis aeruginosa using a gold immunoelectrode modified with antibodies, gold nanoparticles and polytyramine

Lebogang, Lesedi; Mattìasson, Bo; Hedström, Martin

doi:10.1007/s00604-014-1199-4

Cited by 29 publications

(13 citation statements)

References 35 publications

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“…AntiBSA is the specific antibody for BSA and it is characterized by a molecular weight of 160 kDa, more than double that of BSA. Finally, in order to regenerate the sensor, at the end of every experiment, we flowed glycine, pH 2.5, at a concentration of 10 mM in PBS in order to break the interaction between protein A and antiBSA [ 29 ]. Glycine buffer solution, 100 mM, pH 2–2.5 is a solution commonly used for eluting antibodies in various antibody affinity purification procedures, such as protein A-agarose and protein G-agarose [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer

Caroselli

Castelló

Escorihuela

et al. 2018

Sensors

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A proper antibody immobilization on a biosensor is a crucial step in order to obtain a high sensitivity to be able to detect low target analyte concentrations. In this paper, we present an experimental study of the immobilization process of antibodies as bioreceptors on a photonic ring resonator sensor. A protein A intermediate layer was created on the sensor surface in order to obtain an oriented immobilization of the antibodies, which enhances the interaction with the target antigens to be detected. The anti-bovine serum albumin (antiBSA)-bovine serum albumin (BSA) pair was used as a model for our study. An opto-fluidic setup was developed in order to flow the different reagents and, simultaneously, to monitor in real-time the spectral response of the photonic sensing structure. The antiBSA immobilization and the BSA detection, their repeatability, and specificity were studied in different conditions of the sensor surface. Finally, an experimental limit of detection for BSA recognition of only 1 ng/mL was obtained.

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Section: Methodsmentioning

confidence: 99%

Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer

Caroselli

Castelló

Escorihuela

et al. 2018

Sensors

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show abstract

Section: Transducer Functionalization and Characterizationmentioning

confidence: 99%

“…The electrode was then rinsed with 10 mM phosphate buffer saline, stored in buffer, and placed in the refrigerator until further use. The total thickness of the TGA/EDC/NHSS/MCLR mAb/BSA formation on the gold patterned electrode was ~7 nm, based on the expected thicknesses of each layer [26][27][28]. Cyclic voltammetry was used to characterize the surface chemistry functionalization by measuring the current that developed in the electrochemical cell from the WaveNow xv Potentiostat/Galvanostat System (Pine Research Instrumentation Inc).…”

Section: Transducer Functionalization and Characterizationmentioning

confidence: 99%

AC and DC Differential Bridge Structure Suitable for Electrochemical Interfacial Capacitance Biosensing Applications

et al. 2020

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This paper presents a capacitive differential bridge structure with both AC and DC excitation and balancing capability for low cost electrode-solution interfacial capacitance biosensing applications. The proposed series RC balancing structure offers higher sensitivity, lower susceptibility to common-mode interferences, and drift control. To evaluate the bridge performance in practice, possible effects of initial bridge imbalance due to component mismatches are investigated considering the required resolution of the balancing networks, sensitivity, and linearity. This evaluation is also a guideline to designing the balancing networks, balancing algorithm and the proceeding readout interface circuitry. The proposed series RC bridge structure is implemented along with a custom single frequency real-time amplification/filtering readout board with real-time data acquisition and sine fitting. The main specifications for the implemented structure are 8-bit detection resolution if the total expected fractional capacitance change at the interface is roughly 1%. The characterization and measurement results show the effectiveness of the proposed structure in achieving the design target. The implemented structure successfully achieves distinct detection levels for tiny total capacitance change at the electrode-solution interface, utilizing Microcystin-(Leucine-Arginine) toxin dilutions as a proof of concept.

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“…These capacitive biosensors are the electrochemical sensors that measure changes in the dielectric properties when an analyte interacts with a biorecognition element on the sensor surface, causing a decrease in the capacitance 2 4 . Capacitive biosensors have been used for the detection of various analytes like antigens, antibodies, proteins, and heavy metal ions 6 26 27 28 . These types of biosensors have many advantages like inherent rapidity, high sensitivity, simplicity, low cost, easy manipulation, and real-time measurement without labeling 29 .…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ertürk

Lood

2018

JoVE

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The ability to detect and quantitate biomolecules in complex solutions has always been highly sought-after within natural science; being used for the detection of biomarkers, contaminants, and other molecules of interest. A commonly used technique for this purpose is the Enzyme-linked Immunosorbent Assay (ELISA), where often one antibody is directed towards a specific target molecule, and a second labeled antibody is used for the detection of the primary antibody, allowing for the absolute quantification of the biomolecule under study. However, the usage of antibodies as recognition elements limits the robustness of the method; as does the need of using labeled molecules. To overcome these limitations, molecular imprinting has been implemented, creating artificial recognition sites complementary to the template molecule, and obsoleting the necessity of using antibodies for initial binding. Further, for even higher sensitivity, the secondary labeled antibody can be replaced by biosensors relying on the capacitance for the quantification of the target molecule. In this protocol, we describe a method to rapidly and label-free detect and quantitate low-abundant biomolecules (proteins and viruses) in complex samples, with a sensitivity that is significantly better than commonly used detection systems such as the ELISA. This is all mediated by molecular imprinting in combination with a capacitance biosensor.

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Capacitive sensing of microcystin variants of Microcystis aeruginosa using a gold immunoelectrode modified with antibodies, gold nanoparticles and polytyramine

Cited by 29 publications

References 35 publications

Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer

Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer

AC and DC Differential Bridge Structure Suitable for Electrochemical Interfacial Capacitance Biosensing Applications

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

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