Capacity of octacalcium phosphate to promote osteoblastic differentiation toward osteocytes in vitro

Sai, Yuko; Shiwaku, Yukari; Anada, Takahisa; Tsuchiya, Kaori; Takahashi, Tsuneo; Сузукі, Осаму

doi:10.1016/j.actbio.2018.01.026

Cited by 67 publications

(44 citation statements)

References 57 publications

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“…Several lines of evidence have confirmed that the appearance of octacalcium phosphate phase (OCP) during hydroxyapatite (HA) formation increases osteoblast activity if it is present near the surface of OCP. [1][2][3] Observations that partial hydrolysis of OCP enhances bone formation more than that of the original salt supports this proposition. 4 Analyses of OCP under physiological conditions have shown that OCP induces dynamic physicochemical processes, including inorganic ion exchange, 5,6 serum proteins adsorption, [7][8][9] and a progressive crystallographic change from OCP to HA without the involvement of phases other than OCP and HA.…”

Section: Introductionmentioning

confidence: 74%

“…As reported in a previous study, the central peak at 1077 cm −1 has been shown to be obscured when OCP hydrolysis advances and becomes more apatitic. 1,3 However, the FTIR analysis did not show clear changes of spectral features in HA20OCP80 and OCP after osteoclast culture, even though there was XRD evidence of advancement of the hydrolysis (Fig. 6).…”

Section: Changes Of Crystal Structures Of Cap Disksmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

“…[Ca],[P], and pH were used. The calculation also assumed the presence of HCO3 − in the media. The ion pairs consid-DS was defined in terms of their mean ionic activity products with respect to OCP, HA, DCPD, and β-TCP.…”

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confidence: 99%

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Effect of calcium phosphate phases affecting the crosstalk between osteoblasts and osteoclasts in vitro

Shiwaku

Tsuchiya

Xiao

et al. 2019

J Biomedical Materials Res

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Previous studies have reported that octacalcium phosphate (OCP) enhances osteoblast differentiation and osteoclast formation during the hydrolysis process to hydroxyapatite (HA). However, the crystal phases that affect the crosstalk between osteoclasts and osteoblasts are unknown, which should determine the bone substitute material's property of OCP. The present study was designed to investigate whether the chemical composition and crystal structure of calcium phosphates affect osteoclast formation and the osteoclast–osteoblast crosstalk. Biodegradable β‐tricalcium phosphate (β‐TCP) was used as the control material. Osteoclasts were cultured on HA/OCP or HA/TCP disks and their cellular responses were assessed. Both OCP and β‐TCP had a similar ability to create multinucleated osteoclasts. However, OCP promoted the expression of complement component 3a (C3a), a positive coupling factor, in osteoclasts, whereas β‐TCP enhanced that of EphrinB2 (EfnB2) and collagen triple helix repeat containing 1 (Cthrc1). During osteoclast culture, phosphate ions were released from the crystals, and OCP‐HA conversion was advanced in HA/OCP mixtures and OCP. X‐ray diffraction analysis revealed no remarkable changes in the crystal structures of HA/TCP mixtures and β‐TCP before and after osteoclast culture. These results indicate that the distinct chemical environment induced by the calcium phosphate phases affects the crosstalk between osteoclasts and osteoblasts. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1001–1013, 2019.

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Section: Introductionmentioning

confidence: 74%

Section: Changes Of Crystal Structures Of Cap Disksmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

mentioning

confidence: 99%

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Effect of calcium phosphate phases affecting the crosstalk between osteoblasts and osteoclasts in vitro

Shiwaku

Tsuchiya

Xiao

et al. 2019

J Biomedical Materials Res

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“…However, little is known about the interaction between the osteocytes and Ca-Ps. In order to investigate the effect of OCP and other Ca-Ps on the transition of osteoblasts to late osteocytes, mouse IDG-SW3 cells were cultured in transwells with OCP, commercially available β-TCP, or HA 122) . OCP increased the activity of ALP, an osteoblastic marker, on IDG-SW3 cells, compared to β-TCP and HA.…”

Section: Osteocytesmentioning

confidence: 99%

Octacalcium phosphate bone substitute materials: Comparison between properties of biomaterials and other calcium phosphate materials

2020

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Octacalcium phosphate (OCP) is a material that can be converted to hydroxyapatite (HA) under physiological environments and is considered a mineral precursor to bone apatite crystals. The structure of OCP consists of apatite layers stacked alternately with hydrated layers, and closely resembles the structure of HA. The performance of OCP as a bone substitute differs from that of HA materials in terms of their osteoconductivity and biodegradability. OCP manifests a cellular phagocytic response through osteoclastlike cells similar to that exhibited by the biodegradable material β-tricalcium phosphate (β-TCP). The use of OCP for human cranial bone defects involves using its granule or composite form with one of the natural polymers, viz., the reconstituted collagen. This review article discusses the differences and similarities in these calcium phosphate (Ca-P)-based materials from the viewpoint of the structure and their material chemistry, and attempts to elucidate why CaP materials, particularly OCP, display unique osteoconductive property.

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A Graded, Porous Composite of Natural Biopolymers and Octacalcium Phosphate Guides Osteochondral Differentiation of Stem Cells

Amann

Amirall

Franco

et al. 2021

Adv Healthcare Materials

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Lesions involving the osteochondral unit are difficult to treat. Biomimetic scaffolds are previously shown as promising alternative. Such devices often lack multiple functional layers that mimic bone, cartilage, and the interface. In this study, multilayered scaffolds are developed based on the use of natural extracellular matrix (ECM)‐like biopolymers. Particular attention is paid to obtain a complex matrix that mimics the native osteochondral transition. Porous, sponge‐like chitosan‐collagen‐octacalcium phosphate (OCP) scaffolds are obtained. Collagen content increases while the amount of OCP particles decreases toward the cartilage layer. The scaffolds are bioactive as a mineral layer is deposited containing hydroxyapatite at the bony side. The scaffolds stimulate proliferation of human adipose‐derived mesenchymal stem cells, but the degree of proliferation depends on the cell seeding density. The scaffolds give rise to a zone‐specific gene expression. RUNX2, COL1A1, BGLAP, and SPP1 are upregulated in the bony layer of the scaffold. SOX9 is upregulated concomitant with COL2A1 expression in the cartilage zone. Mineralization in presence of the cells is prominent in the bone area with Ca and P steadily increasing over time. These results are encouraging for the fabrication of biomimetic scaffolds using ECM‐like materials and featuring gradients that mimic native tissues and their interface.

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Capacity of octacalcium phosphate to promote osteoblastic differentiation toward osteocytes in vitro

Cited by 67 publications

References 57 publications

Effect of calcium phosphate phases affecting the crosstalk between osteoblasts and osteoclasts in vitro

Effect of calcium phosphate phases affecting the crosstalk between osteoblasts and osteoclasts in vitro

Octacalcium phosphate bone substitute materials: Comparison between properties of biomaterials and other calcium phosphate materials

A Graded, Porous Composite of Natural Biopolymers and Octacalcium Phosphate Guides Osteochondral Differentiation of Stem Cells

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