Capillary electrokinetic chromatography with a suspension of Chromatographic particles

Bächmann, K.; Göttlicher, B.; Haag, I.; Han, K.-Y.; Hensel, Wolfgang; Mainka, A.

doi:10.1016/0021-9673(94)00853-1

Cited by 38 publications

(26 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Carney et al [5] have recently detailed the role of frits in outgassing and how to minimize the problem by silanisation of the frits. Other potential solutions to the problem of adverse frit effects and the general fragility of CEC columns involve the use of in situ generated polymeric (monolithic) columns [6±8], fused and rederivatized octadecylated silica particles [9], suspended chromatographic particles [10] or a replaceable charged polymer [11]. Such methods have wide scope for development but have yet to become widely adopted.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrochromatography for pesticide analysis: Effects of environmental matrices

Cooper¹,

Jessop²,

Moffatt³

2000

Electrophoresis

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Capillary electrochromatography for pesticide analysis: Effects of environmental matrices

Cooper¹,

Jessop²,

Moffatt³

2000

Electrophoresis

View full text Add to dashboard Cite

“…In the first demonstration of this technique, Bächmann et al [9] used 1.5 mm reversed-phase particles coated with SDS to separate nine phenols. The surfactant was necessary to impart some charge and consequently electrophoretic mobility to the particles so that the analytes could be separated by their selective interactions with the stationary phase.…”

Section: Introductionmentioning

confidence: 98%

Manipulation of separation selectivity for alkali metals and ammonium in ion-exchange capillary electrochromatography using a suspension of cation exchange particles in the electrolyte as a pseudostationary phase

Breadmore

Macka²,

Haddad³

1999

Electrophoresis

View full text Add to dashboard Cite

The viability of using ion‐exchange particles as a pseudostationary phase in capillary electrochromatography for the separation of monovalent inorganic cations has been investigated. Using sulfonated polymeric particles (average diameter 225 nm) as the pseudostationary phase, the separation selectivity for alkali metals and ammonium was examined under a range of background electrolyte compositions and employing indirect absorbance detection. Addition of ion‐exchange particles to the background electrolyte resulted in a reduction in the observed electrophoretic mobility of the analytes due to the establishment of ion‐exchange interaction with the pseudostationary phase, with the decrease in mobilities following the ion‐exchange interaction order for these analytes with a sulfonated stationary phase. Increasing the concentration of the particles resulted in a uniform reduction in the electrophoretic mobility of the analytes, similar to that observed in micellar electrokinetic chromatography. Conversely, increasing the concentration of the cationic indirect detection probe (which also acted as an ion‐exchange competing cation) resulted in a decrease in the ion‐exchange interaction with the particles and a reduction of the relative ion‐exchange contribution to the overall separation mechanism. Plots of log[retention factor] versus log[electrolyte concentration] were linear, as is the case for ion‐exchange chromatography, but the observed slopes were greater than predicted from ion‐exchange theory. Indirect absorbance detection was found to give poor sensitivity due to light scattering effects caused by the particles of pseudostationary phase.

show abstract

“…Since the early separations of uncharged molecules with CE using micellar electrokinetic chromatography (MEKC) introduced by Terabe et al [1], various organic molecular aggregates, such as micelles [2±4] and microemulsions (MEEKC) [5±7] have been studied as chromatographic carriers. Discrete molecules such as oligomerized sodium-10-undecylenate [8], cyclodextrins [9,10], starbust dendrimers [11], ionic polymers [12,13] or chromatographic particles [14] have also been used as pseudostationary phases in CE, offering distinct advantages over micelles, such as higher stability with various electrolyte compositions, specific selectivities, or higher efficiencies. Due to the high diversity of separation problems and analytical requirements, research in the field of EKC and pseudostationary phases is still continuing.…”

Section: Introductionmentioning

confidence: 99%

Discontinuous electrokinetic chromatography of parabens using different substituted resonances as pseudostationary phases

et al. 1999

View full text Add to dashboard Cite

Capillary electrokinetic chromatography with a suspension of Chromatographic particles

Cited by 38 publications

References 13 publications

Capillary electrochromatography for pesticide analysis: Effects of environmental matrices

Capillary electrochromatography for pesticide analysis: Effects of environmental matrices

Manipulation of separation selectivity for alkali metals and ammonium in ion-exchange capillary electrochromatography using a suspension of cation exchange particles in the electrolyte as a pseudostationary phase

Discontinuous electrokinetic chromatography of parabens using different substituted resonances as pseudostationary phases

Contact Info

Product

Resources

About