Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009–2014)

Durney, Brandon C.; Crihfield, Cassandra L.; Holland, Lisa

doi:10.1007/s00216-015-8703-5

Cited by 71 publications

(45 citation statements)

References 176 publications

(173 reference statements)

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“…50 Minor modifications to instruments for genome sequencing have adapted these instruments to profiling oligosaccharides using photometric detectors. 51 The high separation efficiency and resolution of CE opens the possibility of high throughput if the method can be robustly coupled to MS.…”

Section: Methods For Profiling Glycans and Glycoprotein Cancer Markersmentioning

confidence: 99%

Recent Advances in the Mass Spectrometry Methods for Glycomics and Cancer

Kailemia

Wong

et al. 2017

Anal. Chem.

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Section: Methods For Profiling Glycans and Glycoprotein Cancer Markersmentioning

confidence: 99%

Recent Advances in the Mass Spectrometry Methods for Glycomics and Cancer

Kailemia

Wong

et al. 2017

Anal. Chem.

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“…Since the development of CE, many researchers have been attracted to it for various applications, especially for analyzing DNA (Durney and others ). However, for more than 30 y, CE has been used primarily in laboratory research rather than in industrial and commercial applications.…”

Section: Limitations Advantages and Future Development Of Ce‐based mentioning

confidence: 99%

Capillary Electrophoresis Based on Nucleic Acid Detection as Used in Food Analysis

Lian

Zeng

2017

Comp Rev Food Sci Food Safe

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Food safety and food production are closely related to the health of consumers. Food-related accidents often cause tremendous losses of personnel and property. Thus, rapid detection and analysis of ingredients in food, tracing food sources, studying the optimal conditions for food production, and more are vital for preventing incidents related to safety. Conventional analysis based on proteomics, microbial cultures, and morphology, as well as biochemical tests based on metabonomics, are considered gold standards and used frequently, but they are labor-intensive, time-consuming, tedious, error-prone, and incapable of meeting the demand for rapid and precise detection at a large scale. Alternative detection methods that utilize capillary electrophoresis have the advantages of high efficiency, high throughput, high speed, and automation; these methods are coupled with various nucleic acid detection strategies to overcome the drawbacks of traditional identification methods, and to prevent false results. Therefore, this review focuses on the application of capillary electrophoresis based on nucleic acid detection in food analysis and provides an introduction to the limitations, advantages, and future developments of this approach.

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“…CE is employed in diverse applications including DNA and protein separation [3–5], detection of disease biomarkers [6,7], environment monitoring on earth and other planets [8,9], food (e.g. wine) analysis [10], and pharmaceutical analysis [11–13].…”

Section: Introductionmentioning

confidence: 99%

Novel volumetric method for highly repeatable injection in microchip electrophoresis

Jones

et al. 2017

Analytica Chimica Acta

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A novel injector for microchip electrophoresis (MCE) has been designed and evaluated that achieves very high repeatability of injection volume suitable for quantitative analysis. It eliminates the injection biases in electrokinetic injection and the dependence on pressure and sample properties in hydrodynamic injection. The microfluidic injector, made of poly(dimethylsiloxane) (PDMS), operates similarly to an HPLC injection valve. It contains a channel segment (chamber) with a well-defined volume that serves as an “injection loop”. Using on-chip microvalves, the chamber can be connected to the sample source during the “loading” step, and to the CE separation channel during the “injection” step. Once the valves are opened in the second state, electrophoretic potential is applied to separate the sample. For evaluation and demonstration purposes, the microinjector was connected to a 75 µm ID capillary and UV absorbance detector. For single compounds, a relative standard deviation (RSD) of peak area as low as 1.04% (n=11) was obtained, and for compound mixtures, RSD as low as 0.40% (n=4) was observed. Using the same microchip, the performance of this new injection technique was compared to hydrodynamic injection and found to have improved repeatability and less dependence on sample viscosity. Furthermore, a non-radioactive version of the positron-emission tomography (PET) imaging probe, FLT, was successfully separated from its known 3 structurally-similar byproducts with baseline resolution, demonstrating the potential for rapid, quantitative analysis of impurities to ensure the safety of batches of short-lived radiotracers. Both the separation efficiency and injection repeatability were found to be substantially higher when using the novel volumetric injection approach compared to electrokinetic injection (performed in the same chip). This novel microinjector provides a straightforward way to improve the performance of hydrodynamic injection and enables extremely repeatable sample volume injection in MCE. It could be used in any MCE application where volume repeatability is needed, including the quantitation of impurities in pharmaceutical or radiopharmaceutical samples.

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Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009–2014)

Cited by 71 publications

References 176 publications

Recent Advances in the Mass Spectrometry Methods for Glycomics and Cancer

Recent Advances in the Mass Spectrometry Methods for Glycomics and Cancer

Capillary Electrophoresis Based on Nucleic Acid Detection as Used in Food Analysis

Novel volumetric method for highly repeatable injection in microchip electrophoresis

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