Capillary Electrophoresis as a Monitoring Tool for Flow Composition Determination

Abstract

Flow analysis is the science of performing quantitative analytical chemistry in flowing streams. Because of its efficiency and speed of analysis, capillary electrophoresis (CE) is a prospective method for the monitoring of a flow composition withdrawn from various processes (e.g., occurring in bioreactors, fermentations, enzymatic assays, and microdialysis samples). However, interfacing CE to a various flow of interest requires further study. In this paper, several ingenious approaches on interfacing flow from various chemical or bioprocesses to a capillary electrophoresis instrument are reviewed. Most of these interfaces can be described as computer-controlled autosamplers. Even though most of the described interfaces waste too many samples, many interesting and important applications of the devices are reported. However, the lack of commercially available devices prevents the wide application of CE for flow analysis. On the contrary, this fact opens up a potential avenue for future research in the field of flow sampling by CE.

A gravity‐independent single‐phase electrode reservoir for capillary electrophoresis applications

“…Applied HV can short the fluidic path to electrical ground, damaging system components if the HV fluidic system is not properly isolated from the rest of the instrument fluidic components. Flow-through reservoirs have been used in previous portable CE systems [14][15][16], but the problem of HV isolation of the fluidic path was avoided by manually decoupling the system ahead of HV application [16], using lower HV levels [15], or by employing a liquid-distribution mechanism only at the ground side [14][15][16][17]. No previous work has demonstrated a HV compatible flow-through reservoir for an automated CE system.…”