Capillary Electrophoresis as Tool for Diastereomeric Separation in a<i>Trichilia catigua</i>Fraction

Sereia, Ana Luiza; Longhini, Renata; Lopes, Gisely Cristiny; Mello, João Carlos Palazzo de

doi:10.1002/pca.2653

Cited by 12 publications

(10 citation statements)

References 27 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As we observed in another study [32], the capillary electrophoresis methodologies developed in this work were faster, less expensive and less polluting than the equivalent HPLC methods currently in use [21,26].…”

Section: Resultssupporting

confidence: 53%

“…Our research group also published a methodology using capillary electrophoresis to analyse the EAF of T . catigua [32].…”

Section: Resultsmentioning

confidence: 99%

“…pluviosa and S . adstringens was performed following the steps previously described by our research group [32].…”

Section: Resultsmentioning

confidence: 99%

“…An appropriate amount of each EAF was weighed, dissolved in 10.0 mL of a 20% methanol solution and eluted through a solid-phase extraction (SPE) cartridge (Strata C18-E, Phenomenex), as described previously [32]. All solutions for analysis were filtered through a 0.45 μm Millipore filter.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

et al. 2019

Self Cite

View full text Add to dashboard Cite

Alzheimer’s disease (AD) is the most common form of dementia and has no cure. Therapeutic strategies focusing on the reduction of oxidative stress, modulation of amyloid-beta (Aβ) toxicity and inhibition of tau protein hyperphosphorylation are warranted to avoid the development and progression of AD. The aim of this study was to screen the crude extracts (CEs) and ethyl-acetate fractions (EAFs) of Guazuma ulmifolia , Limonium brasiliense , Paullinia cupana , Poincianella pluviosa , Stryphnodendron adstringens and Trichilia catigua using preliminary in vitro bioassays (acetylcholinesterase inhibition, antioxidant activity and total polyphenol content) to select extracts/fractions and assess their protective effects against Aβ 25–35 toxicity in SH-SY5Y cells. The effect of the EAF of S . adstringens on mitochondrial membrane potential, lipid peroxidation, superoxide production and mRNA expression of 10 genes related to AD was also evaluated and the electropherogram fingerprints of EAFs were established by capillary electrophoresis. Chemometric tools were used to correlate the in vitro activities of the samples with their potential to be evaluated against AD and to divide extracts/fractions into four clusters. Pretreatment with the EAFs grouped in cluster 1 ( S . adstringens , P . pluviosa and L . brasiliense ) protected SH-SY5Y cells from Aβ 25-35 -induced toxicity. The EAF of S . adstringens at 15.62 μg/mL was able completely to inhibit the mitochondrial depolarization (69%), superoxide production (49%) and Aβ 25-35 -induced lipid peroxidation (35%). With respect to mRNA expression, the EAF of S . adstringens also prevented the MAPT mRNA overexpression (expression ratio of 2.387x) induced by Aβ 25–35 , which may be related to tau protein hyperphosphorylation. This is the first time that the neuroprotective effects of these fractions have been demonstrated and that the electropherogram fingerprints for the EAFs of G . ulmifolia , L . brasiliense , P . cupana , P . pluviosa and S . adstringens have been established. The study expands knowledge of the in vitro protective effects and quality control of the evaluated fractions.

show abstract

Section: Resultssupporting

confidence: 53%

“…Our research group also published a methodology using capillary electrophoresis to analyse the EAF of T . catigua [32].…”

Section: Resultsmentioning

confidence: 99%

“…pluviosa and S . adstringens was performed following the steps previously described by our research group [32].…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, they developed a CZE method with dual CDs as additives for the separation and determination of uncaria alkaloids in Uncaria samples . HP‐β‐CD was also used to improve the separation of diastereomeric pairs, the separation of catechin, epicatechin, cinchonains Ia, Ib, IIa, and IIb from Trichilia catigua samples on the same run could be realized in less than 12 min by using voltage reduction . In our previous study, a simple CZE method with an IL, BMImBF 4 as an additive was developed for the simultaneous determination of eight isoflavones including ononin, daidzin, genistin, biochanin A, formononetin, puerarin, genistein and daizein in Radix Puerariae .…”

Section: Methods Developmentmentioning

confidence: 99%

Capillary electrophoresis in phytochemical analysis (2014–2017)

Zhou

Zhang

2018

Separation Science Plus

View full text Add to dashboard Cite

This updated review is focused on the applications of CE in phytochemical analysis during the period of 2014 to April 2018 (about 130 papers were included). The article mainly consists of three main sections, including sample pretreatment (extraction and on‐line pre‐concentration), method development (separation and detection) and applications in quality evaluation of medicinal plants, screening of active compounds (enzyme inhibitors) and studying interaction between phytochemicals and bio‐macromolecules.

show abstract

Alternative method for microenzymatic inhibition activities monitoring fromBaccharis trimeraextracts by CZE‐UV

Campos

Lima

Campos

et al. 2022

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction: Capillary zone electrophoresis with direct UV detection (CZE-UV) was used to investigate the hypothesis about the extract of Baccharis trimera enzymatic activities as an analytical approach to monitoring the phenomenon.Objective: The aim of this work was to investigate enzymatic bioactivities of the hydroalcoholic and infusion extracts of B. trimera through screening evaluation of the inhibition of the enzymes acetylcholinesterase (AChE) and α-glycosidase (α-GLY).Method: An alternative approach using CZE-UV to hydroalcoholic and infusion extracts of B. trimera monitoring was applied to evaluate the inhibition ability of the enzymes AChE and α-GLY. The result of the reaction of acetylthiocholine (AThCh) with AChE was thiocholine (TCh) and acetic acid, and from the amount of TCh generated, the AChE inhibition was calculated. For the inhibition study of the two enzymes, the reactions of the extracts were optimised to be performed in situ, inside the capillary column, and the introduction of the solutions was performed through ordered sequential plug injections.Results: Samples extracted with 70% ethanol presented 7.80% inhibition for AChE and 0.51% for α-GLY, while samples extracted with 96% ethanol resulted in 6.89% inhibition for AChE and no inhibition activity for α-GLY. Conclusion:In the present work, the potentialities of CZE-UV for the study of hydroalcoholic and infusion extracts of B. trimera were demonstrated. The experimental results were useful for the calculation of the percentage of the inhibition activities of the AChE and α-GLY enzymes.

show abstract

Capillary Electrophoresis as Tool for Diastereomeric Separation in aTrichilia catiguaFraction

Abstract: Our results demonstrate that this method could be employed in a quality-control laboratory for the quantification of polyphenols in EAF of Trichilia catigua. Copyright © 2016 John Wiley & Sons, Ltd.

Cited by 12 publications

References 27 publications

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Capillary electrophoresis in phytochemical analysis (2014–2017)

Alternative method for microenzymatic inhibition activities monitoring fromBaccharis trimeraextracts by CZE‐UV

Contact Info

Product

Resources

About