Capillary electrophoresis based on nucleic acid detection for diagnosing human infectious disease

Abstract

Rapid transmission, high morbidity, and mortality are the features of human infectious diseases caused by microorganisms, such as bacteria, fungi, and viruses. These diseases may lead within a short period of time to great personal and property losses, especially in regions where sanitation is poor. Thus, rapid diagnoses are vital for the prevention and therapeutic intervention of human infectious diseases. Several conventional methods are often used to diagnose infectious diseases, e.g. methods based on cultures or morphology, or biochemical tests based on metabonomics. Although traditional methods are considered gold standards and are used most frequently, they are laborious, time consuming, and tedious and cannot meet the demand for rapid diagnoses. Disease diagnosis using capillary electrophoresis methods has the advantages of high efficiency, high throughput, and high speed, and coupled with the different nucleic acid detection strategies overcomes the drawbacks of traditional identification methods, precluding many types of false positive and negative results. Therefore, this review focuses on the application of capillary electrophoresis based on nucleic detection to the diagnosis of human infectious diseases, and offers an introduction to the limitations, advantages, and future developments of this approach.

Lock-in Amplifier Dairy Sensor for Detection of Ciprofloxacin

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Capillary electrophoresis of PCR fragments with 5´-labelled primers for testing the SARS-Cov-2

“…Moreover, the absence/presence of a PCR-fragment is interpreted by a software that measures the fluorescent intensity, that is proportional to the amount of the target sequence in the sample. FCE has also advantages relative to the qtPCR, such as the rapid and sensitive detection of dozens of different PCR fragments in single runs (Lian and Zhao, 2016). During the first months of the COVID19 pandemic we faced a demand for SARS-Cov-2 testing that exceeded our qtPCR capacity.…”

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

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