Capillary electrophoresis behavior of water-soluble anionic porphyrins in the presence of β-cyclodextrin and itsO-methylated derivatives

Andrighetto, Paolo; Carofiglio, Tommaso; Fornasier, Roberto; Tonellato, Umberto

doi:10.1002/(sici)1522-2683(20000201)21:3<619::aid-elps619>3.0.co;2-y

Cited by 13 publications

(4 citation statements)

References 18 publications

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“…Simple mixtures of sulfonated porphyrins can be separated as a function of charge by capillary electrophoresis [36][37][38]. The sulfonated naphthyl porphyrins are mixtures of numerous compounds, however, making separation more difficult.…”

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Sulfonated naphthyl porphyrins as agents against HIV-1

Dixon

Gill

Giribabu

et al. 2005

Journal of Inorganic Biochemistry

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Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Sulfonated naphthyl porphyrins as agents against HIV-1

Dixon

Gill

Giribabu

et al. 2005

Journal of Inorganic Biochemistry

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“…We used the 5-(p-aminophenyl)-10,15,20-tris(p-sulfonatophenyl) porphyrin, 4, which is water-soluble (due to the three sulfonic groups), and contains a reactive amino group that undergoes a rapid diazotization in the presence of nitrite and may act as an anionic carrier. We have recently reported on the beneficial effect of b-cyclodextrin and its O-methylated derivatives on the concentration zone electrophoresis separation of anionic porphyrins such as meso-tetrakis(4-carboxyphenyl)porphyrin, meso-tetrakis-(4-sulfonatophenyl) and their zinc(II) and copper(II) complexes [18]. Cyclodextrains (CDs) are water-soluble, naturally occurring cyclic oligosaccharides containing 6±8 D-glucose units arranged in such a way as to create a fairly rigid apolar cavity.…”

Section: Introductionmentioning

confidence: 74%

“…Based on our previous results on CE of porphyrins [18], the BGE of choice was a 20 mM phosphate buffer at pH 7.0. Under neutral conditions the three porphyrins 4, 5, and 6 possess total charge of ±3 (neither the pyrrolic nitrogens of the porphyrin core nor the amino group are protonated in neutral solution), ±2 and ±3, respectively.…”

Section: Cementioning

confidence: 99%

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A highly sensitive method for the analysis of nitrite ions by capillary zone electrophoresis using water-soluble aminophenylporphyrin derivative as chromogenic reagent

Andrighetto¹,

Carofiglio²,

Fornasier³

et al. 2000

Electrophoresis

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The water soluble 5-p-aminophenyl)-10,15,20-tris(p-sulfonatophenyl) porphyrin, 4, acts as an extremely efficient chromogenic reagent for the detection of very low amounts of nitrites. The amino group of porphyrin 4 reacts smoothly with nitrite in acidic conditions 0.2 M HCl) producing the corresponding diazo-porphyrin derivative which is stable and does not show any appreciable hydrolysis to phenol within 6 h. The reaction is carried out in the presence of 25 mM heptakis-(2,6-di-O-methyl)-beta-cyclodextrin that prevents precipitation of the protonated form of porphyrins 4 or 5 due to the formation of strong inclusion complexes. The capillary zone electrophoresis of the diazoporphyrin and amino-porphyrin mixture shows severe peak tailing. However, symmetrical peaks can be obtained by adding 5 mM beta-cyclodextrin to the background electrolyte (20 mM phosphate buffer, pH 7.0). Calibration curve for nitrite analysis is linear up to 0.25 mM nitrite and the detection limit (at a signal-to-noise ratio of 3) has been estimated to be a 1 microM (50 ppb) of nitrite concentration in aqueous solutions.

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Capillary electrophoresis separation and native laser-induced fluorescence detection of metallotexaphyrins

Quirino

Dulay

et al. 2002

J. Sep. Science

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Capillary electrophoresis separation and native laser-induced fluorescence detection of metallotexaphyrinsMethods based on capillary zone electrophoresis (CZE) and electrokinetic chromatography (EKC) are developed for the separation of motexafin gadolinium (MGd, Xcytrinm), a novel redox-active drug, under development for cancer therapy, and two closely related metallotexaphyrin impurities, called PCI-0400 and PCI-0430. The three metallotexaphyrins are baseline resolved using EKC in a separation solution containing 400-mM cetyltrimethyl ammonium bromide, 50-mM phosphoric acid, and 20% methanol. Detection is achieved using native laser-induced fluorescence (LIF) by exciting at 488 nm and collecting emission at 750 nm. Analysis of a representative clinical batch of MGd using EKC combined with native LIF allowed for the detection of PCI-0400 and PCI-0430 along with nine other possible MGd impurities, each of which is present at approximately 0.1% (total weight of the sample).

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Capillary electrophoresis behavior of water-soluble anionic porphyrins in the presence of β-cyclodextrin and itsO-methylated derivatives

Cited by 13 publications

References 18 publications

Sulfonated naphthyl porphyrins as agents against HIV-1

Sulfonated naphthyl porphyrins as agents against HIV-1

A highly sensitive method for the analysis of nitrite ions by capillary zone electrophoresis using water-soluble aminophenylporphyrin derivative as chromogenic reagent

Capillary electrophoresis separation and native laser-induced fluorescence detection of metallotexaphyrins

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