Capillary Electrophoresis Coupled To Mass Spectrometry for Forensic Analysis

Huck, Christian; Huck-Pezzei, V. A.; Bakry, Rania; Bachmann, Stefan; Najam‐ul‐Haq, Muhammad; Rainer, Matthias; Bonn, Günther K.

doi:10.2174/1874123100701010030

Cited by 6 publications

(4 citation statements)

References 67 publications

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“…The intraassay precision ranged from 4.1 to 7.3% RSD. The intra-assay accuracy ranged from 94.0 to 104% 13,14 .…”

Section: Determination Of Drugs In Human Plasmamentioning

confidence: 99%

Untitled

2020

IJPSR

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Capillary Electrophoresis-Mass Spectroscopy (CE-MS) is a hyphenated technique as it combines the advantages of both CE and MS. It provides high-resolution separations with high detection selectivity and sensitivity, which allows detailed characterization of many biomolecules in a single analysis. CE-MS improves the ability to deal with the analysis of complex biological samples containing trace amounts of analytes. CE coupled MS has become a widely used analytical technique in fields such as proteomics, metabolomics, and genomics. It is also increasingly used in various other fields like environmental science, food analysis, biotechnology and forensic science etc. However, the development of online CE-MS is not without challenges. Understanding of CE, the interface setup, ionization technique and mass detection system is important to tackle problems while coupling capillary electrophoresis to mass spectrometry. In this article, instrumentation details of CE-MS, the technical aspects of CE-MS interface, applications and the advancements made in recent years are discussed. INTRODUCTION: Capillary electrophoresis (CE) is a versatile technique. The separation is based on their electrophoretic mobility and electroosmotic flow with the use of applied voltage. It was first introduced by HJERTON in the year 1967. CE plays an ideal role in the analysis of highly polar charged analyte, analysis of both basic and clinical pharmaceutical and in analysis and characterization of macromolecules. CE uses tubes 20-100µm diameter and 20-100cm in length. Higher electric fields result in high efficiency and narrow peaks.

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“…The intraassay precision ranged from 4.1 to 7.3% RSD. The intra-assay accuracy ranged from 94.0 to 104% 13,14 .…”

Section: Determination Of Drugs In Human Plasmamentioning

confidence: 99%

Untitled

2020

IJPSR

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“…The low sensitivity and reproducibility of the old CE‐MS, as well as popularity of LC‐MS caused the forensic drug analysts to shy away from CE‐MS. In spite of this CE‐MS was applied to the analysis of a wide range of illicit drug in various matrices .…”

Section: Applications Of Liquid Separation Ms To the Analysis Of Illimentioning

confidence: 99%

Applications of liquid‐based separation in conjunction with mass spectrometry to the analysis of forensic evidence

Moini

2018

Electrophoresis

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In the past few years, there has been a significant effort by the forensic science community to develop new scientific techniques for the analysis of forensic evidence. Forensic chemists have been spearheaded to develop information-rich confirmatory technologies and techniques and apply them to a broad array of forensic challenges. The purpose of these confirmatory techniques is to provide alternatives to presumptive techniques that rely on data such as color changes, pattern matching, or retention time alone, which are prone to more false positives. To this end, the application of separation techniques in conjunction with mass spectrometry has played an important role in the analysis of forensic evidence. Moreover, in the past few years the role of liquid separation techniques, such as liquid chromatography and capillary electrophoresis in conjunction with mass spectrometry, has gained significant tractions and have been applied to a wide range of chemicals, from small molecules such as drugs and explosives, to large molecules such as proteins. For example, proteomics and peptidomics have been used for identification of humans, organs, and bodily fluids. A wide range of HPLC techniques including reversed phase, hydrophilic interaction, mixed-mode, supercritical fluid, multidimensional chromatography, and nanoLC, as well as several modes of capillary electrophoresis mass spectrometry, including capillary zone electrophoresis, partial filling, full filling, and micellar electrokenetic chromatography have been applied to the analysis drugs, explosives, and questioned documents. In this article, we review recent (2015-2017) applications of liquid separation in conjunction with mass spectrometry to the analysis of forensic evidence.

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“…Although many detectors are used in CE, high sensitivity detection of neurodrugs and their metabolites in biological, clinical and forensic samples at ultra-trace level only can be achieved by CE combined with mass spectrometric techniques [108-111]. Recent developments on CE-MS methods for analysis of neurological substances in various samples are discussed below.…”

Section: Capillary Electrophoresis-mass Spectro-metrymentioning

confidence: 99%

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

Kailasa

2013

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Recently, mass spectrometric related techniques have been widely applied for the identification and quantification of neurochemicals and their metabolites in biofluids. This article presents an overview of mass spectrometric techniques applied in the detection of neurological substances and their metabolites from biological samples. In addition, the advances of chromatographic methods (LC, GC and CE) coupled with mass spectrometric techniques for analysis of neurochemicals in pharmaceutical and biological samples are also discussed.

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Capillary Electrophoresis Coupled To Mass Spectrometry for Forensic Analysis

Cited by 6 publications

References 67 publications

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Applications of liquid‐based separation in conjunction with mass spectrometry to the analysis of forensic evidence

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

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