Capillary electrophoresis in chemical/pharmaceutical quality control

Pluym, A.; Ael, Willy Van; Smet, Maurits De

doi:10.1016/0165-9936(92)80116-n

Cited by 50 publications

(7 citation statements)

References 4 publications

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“…However, using HPLC for 8OHdG often employed complicated and time-consuming double or triple column switching [16,18,22] and two or three-step solid phase extraction (SPE) [16,18]. Capillary electrophoresis (CE) has been interested in life sciences due to its high efficiency, high speed, and small sample size requirement [23][24][25][26][27]. Combination of electrochemical detection with capillary electrophoresis (CE-ECD) will be more suitable to analyze complex biological samples.…”

Section: Introductionmentioning

confidence: 99%

Determination of urinary oxidative DNA damage marker 8-hydroxy-2′-deoxyguanosine and the association with cigarette smoking

Yao

Mei

Weng

et al. 2004

Talanta

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Section: Introductionmentioning

confidence: 99%

Determination of urinary oxidative DNA damage marker 8-hydroxy-2′-deoxyguanosine and the association with cigarette smoking

Yao

Mei

Weng

et al. 2004

Talanta

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“…The influence of the addiCorrespondence: Professor J. Crommen, Laboratory of Drug Analysis, Institute of Pharmacy, University of Liege, rue Fusch 5, B-4000 LiBge, Belgium Abbreviations: BGE, background electrolyte; CD, 8-cyclodextrin; CZE, capillary zone electrophoresis; DMCD, dimethyl-6-cyclodextrin; HPCD, hydroxypropyl-P-cyclodextrin; TMCD, trimethyl-B-cyclodextrin tion of organic solvents, such as methanol and acetonitrile, has been examined in several papers [3,5,131. The effects of other additives, such as methylhydroxyethyl cellulose [6], quaternary ammonium ions [12] and urea [3, 8, 10, 191, as well as of operating parameters such as temperature [7][8][9]191 and field strength [14] on chiral separation by CZE with cyclodextrins have also been studied. Quantitative aspects have been treated by different authors 12, 11, 12, 151.…”

Section: Introductionmentioning

confidence: 99%

Chiral separation of basic drugs by capillary zone electrophoresis with cyclodextrin additives

et al. 1994

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The enantiomers of a series of basic drugs were separated in capillary zone electrophoresis (CZE) in phosphate buffers, pH 3, containing beta-cyclodextrin or one of its derivatives as chiral selectors and uncoated fused silica capillaries thermostated at 15 degrees C. The nature of the cationic component of the background electrolyte was found to have a significant influence on achiral resolution and peak symmetry. The best results were obtained with triethanolamine, which was then used to adjust the buffer pH in all further experiments. The effects on chiral resolution of the nature and concentration of cyclodextrin, of the addition of methanol, and of capillary temperature were studied. Maximum resolution was obtained at a particular cyclodextrin concentration for each analyte, depending on the affinity of the analyte for this cyclodextrin. On the basis of the results, the effects of methanol addition and temperature on enantiomeric resolution could be explained and predicted. Numerous chiral separations are presented and suggestions for the rapid optimization of CZE enantioseparations with cyclodextrin additives are given.

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“…This study complements previous work demonstrating that CZE-ESI-MS is a complementary or alternative technique to chromatography for impurity profiling of drugs. For example, Pluym et al [40] have reported the full profiling of domperidone impurities using a combination of CZE and HPLC, whereas the individual methods did not resolve all the impurities. Similarly in our studies, CZE methods best separated polar analytes (such as mixture B1), while HPLC methods best resolved hydrophobic compounds (such as mixture B2).…”

Section: Cze-esi-ms Analysis Of Test Mixturesmentioning

confidence: 98%

A generic approach to the impurity profiling of drugs using standardised and independent capillary zone electrophoresis methods coupled to electrospray ionisation mass spectrometry

et al. 2005

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Three standardised, capillary zone electrophoresis-electrospray ionisation mass spectrometry (CZE-ESI-MS) methods were developed for the analysis of six drug candidates and their respective process-related impurities comprising a total of 22 analytes with a range of functional groups and lipophilicities. The selected background electrolyte conditions were found to be: 60/40 v/v 10 mM ammonium formate pH 3.5/organic, 60/40 v/v 10 mM ammonium acetate pH 7.0/organic and 10 mM piperidine, pH 10.5, where the organic solvent is 50/50 v/v methanol/acetonitrile. The coaxial sheath flow consisted of either 0.1% v/v formic acid in 50/50 v/v methanol/water, or 10 mM ammonium acetate in 50/50 v/v methanol/water, depending on the mixture being analysed. Factor analysis and informational theory were used to quantify the orthogonality of the methods and predict their complementarities. The three selected CZE-ESI-MS methods allowed the identification of 21 out of 22 of all the drug candidates and their process-related impurities and provided orthogonality with four established high-performance liquid chromatography-mass spectrometry (HPLC-MS) methods. These methodologies therefore form the basis of a generic approach to impurity profiling of pharmaceutical drug candidates and can be applied with little or no analytical method development, thereby offering significant resource and time savings.

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Capillary electrophoresis in chemical/pharmaceutical quality control

Cited by 50 publications

References 4 publications

Determination of urinary oxidative DNA damage marker 8-hydroxy-2′-deoxyguanosine and the association with cigarette smoking

Determination of urinary oxidative DNA damage marker 8-hydroxy-2′-deoxyguanosine and the association with cigarette smoking

Chiral separation of basic drugs by capillary zone electrophoresis with cyclodextrin additives

A generic approach to the impurity profiling of drugs using standardised and independent capillary zone electrophoresis methods coupled to electrospray ionisation mass spectrometry

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