Capillary Electrophoresis in Chiral Separations

Abstract

This chapter starts with an overview of the theory behind capillary electrophoresis and its derived methods. The capillary electrophoresis instrumentation, its separation principle, the theory behind it, and its modes of operation are discussed. This overview is followed by an illustration of the applicability of capillary electrophoresis and its derived techniques in the analysis of chiral compounds using different types of chiral selectors. Some examples are given for each type of chiral selector. Details are provided about the capillaries used, the chiral selectors, and the stationary and mobile phases.

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis

“…In the case of small‐molecule drugs, capillary electromigration techniques have been employed for the analysis of all aspects in drug development and quality control including the determination of the content of an active pharmaceutical ingredient; the analysis of drug‐related impurities, stereochemical purity, organic and inorganic counter ions; or the determination of physicochemical parameters such as p K a values, lipophilicity (log P values), or drug protein binding . Furthermore, CE‐based enzyme assays have been developed for the screening of inhibitory activity in drug discovery as, for example, summarized in .…”

“…These analyses have been summarized elsewhere, for example, in and are not included in the present review either. For earlier compilations of the use of capillary electromigration methods in drug analysis see, for example, .…”

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis (2013–2015)

“…CE is an important technique for analyzing many pharmaceutical and biopharmaceutical substances . Consequently, protocols for method validation and instrument qualification have been developed and CE methods have been applied during all stages of drug discovery as well as quality control of the finished products .…”

“…In pharmaceutical analysis, CE has been applied to the determination of the main component as well as the purity of drugs with regard to related substances and stereoisomeric impurities, of drug counter‐ions, of physicochemical properties such as log P and pK a . Drug‐macromolecule binding properties were studied by ACE .…”

“…These analyses have been summarized, for example, in and are not discussed in the present review either. For earlier compilations of the use of capillary electromigration methods in drug analysis see, for example .…”