Capillary Electrophoresis Mass Spectrometry as A Tool for Untargeted Metabolomics

Garcı́a, Antonia; Godzień, Joanna; López‐Gonzálvez, Ángeles; Barbas, Coral

doi:10.4155/bio-2016-0216

Cited by 72 publications

(44 citation statements)

References 119 publications

(328 reference statements)

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“…A striking aspect of the latter, as already mentioned above, is the recognition that only a thorough screening of as many metabolites as possible can provide useful information on all the possible altered biomolecular pathways to go beyond the mere Aβ cascade described by the amyloid hypothesis of AD. In this perspective, in the attempt to be as comprehensive and open‐minded as possible regarding all possible cofactors that cause AD, untargeted metabolomics has been pursued alongside a targeted one that is, metabolomics investigations without predefining which metabolites should be measured. Analysis of this kind conducted in human plasma revealed alterations in 22 biochemical pathways, in particular polyamine metabolism and L‐arginine metabolism disrupted in mild cognitive impairment subjects converting to AD .…”

Section: Beyond the Aβ Peptides: The Contribution Of Mass Spectrometrmentioning

confidence: 99%

Mass spectrometry is a multifaceted weapon to be used in the battle against Alzheimer's disease: Amyloid beta peptides and beyond

Grasso

2018

Mass Spectrometry Reviews

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Amyloid-β peptide (Aβ) accumulation and aggregation have been considered for many years the main cause of Alzheimer's disease (AD), and therefore have been the principal target of investigation as well as of the proposed therapeutic approaches (Grasso [2011] Mass Spectrom Rev. 30: 347-365). However, the amyloid cascade hypothesis, which considers Aβ accumulation the only causative agent of the disease, has proven to be incomplete if not wrong. In recent years, actors such as metal ions, oxidative stress, and other cofactors have been proposed as possible co-agents or, in some cases, main causative factors of AD. In this scenario, MS investigation has proven to be fundamental to design possible diagnostic strategies of this elusive disease, as well as to understand the biomolecular mechanisms involved, in the attempt to find a possible therapeutic solution. We review the current applications of MS in the search for possible Aβ biomarkers of AD to help the diagnosis of the disease. Recent examples of the important contributions that MS has given to prove or build theories on the molecular pathways involved with such terrible disease are also reviewed.

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Section: Beyond the Aβ Peptides: The Contribution Of Mass Spectrometrmentioning

confidence: 99%

Mass spectrometry is a multifaceted weapon to be used in the battle against Alzheimer's disease: Amyloid beta peptides and beyond

Grasso

2018

Mass Spectrometry Reviews

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“…The increasing data showing the strong capabilities of CE emphasizes the need of a review in the art of gut microbiota metabolomics analysis based on this technique. Indeed, according to the literature, CE as separation technique for gut microbiota metabolomics analysis is used by less than 5% of the studies (Table ), although this number is expected to increase.…”

Section: Ce As Complementary Metabolomics Tool: Some Considerationsmentioning

confidence: 99%

Phenotyping of gut microbiota: Focus on capillary electrophoresis

et al. 2017

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The analysis of the microbial metabolome is crucial to fully understand the symbiotic relation between humans and microbes. That is why an explosion of metabolomics took place in the area. So far, at least several hundreds of microbial metabolites have been shown to be statistically altered when humans undergo a plethora of commonly faced perturbations. NMR and MS, usually coupled with GC, LC and CE have revealed their identities. CE is a robust analytical platform for the analysis of polar and ionic metabolites that are essential in order to assess the cells' activity. Due to its novelty, only 5% of the metabolomics studies investigate gut microbiota using CE, even though the metabolites found by CE as being significantly altered in human microbiota represent around 23% of the total number of metabolites identified by metabolomics tools. Herein, we discuss the advances of metabolomics in the frame of other OMICS techniques for human gut microbiota analysis. Afterwards, we focus on sample treatment, analytical methods and data processing in CE coupled to any detector that have been reported to date in order to enhance metabolite coverage in the art, and to identify metabolite markers that cannot be covered by other platforms but are of key importance for determining microbial activity and human health.

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“…It provides complementary metabolic information compared to chromatography‐based techniques. Until now, CE coupled to MS has been utilized for metabolic profiling of a wide range of biological samples in various application fields . However, in comparison to other analytical techniques the use of CE–MS in metabolomics is still underrepresented .…”

Section: Introductionmentioning

confidence: 99%

Assessing the suitability of capillary electrophoresis‐mass spectrometry for biomarker discovery in plasma‐based metabolomics

et al. 2019

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The actual utility of capillary electrophoresis‐mass spectrometry (CE‐MS) for biomarker discovery using metabolomics still needs to be assessed. Therefore, a simulated comparative metabolic profiling study for biomarker discovery by CE‐MS was performed, using pooled human plasma samples with spiked biomarkers. Two studies have been carried out in this work. Focus of study I was on comparing two sets of plasma samples, in which one set (class I) was spiked with five isotope‐labeled compounds, whereas another set (class II) was spiked with six different isotope‐labeled compounds. In study II, focus was also on comparing two sets of plasma samples, however, the isotope‐labeled compounds were spiked to both class I and class II samples but with concentrations which differ by a factor two between both classes (with one compound absent in each class). The aim was to determine whether CEMS‐based metabolomics could reveal the spiked biomarkers as the main classifiers, applying two different data analysis software tools (MetaboAnalyst and Matlab). Unsupervised analysis of the recorded metabolic profiles revealed a clear distinction between class I and class II plasma samples in both studies. This classification was mainly attributed to the spiked isotope‐labeled compounds, thereby emphasizing the utility of CE‐MS for biomarker discovery.

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Capillary Electrophoresis Mass Spectrometry as A Tool for Untargeted Metabolomics

Cited by 72 publications

References 119 publications

Mass spectrometry is a multifaceted weapon to be used in the battle against Alzheimer's disease: Amyloid beta peptides and beyond

Mass spectrometry is a multifaceted weapon to be used in the battle against Alzheimer's disease: Amyloid beta peptides and beyond

Phenotyping of gut microbiota: Focus on capillary electrophoresis

Assessing the suitability of capillary electrophoresis‐mass spectrometry for biomarker discovery in plasma‐based metabolomics

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