Capillary Electrophoresis of Nucleotides on Ucon-Coated Fused Silica Columns

O’Neill, Kim L.; Shao, Xiaowen; Zhao, Zhiwei; Malik, Abdul; Lee, M. L.

doi:10.1006/abio.1994.1471

Cited by 37 publications

(32 citation statements)

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“…All solutes could be separated within 16 rain with baseline resolution. This represents less than half of the analysis time previously reported [23,24]. Figure 2 shows the calculated retention factors on various columns according to Eq.…”

Section: Effect Of Sample-wall Interaction On Migration Speedmentioning

confidence: 98%

“…Accurate identification and quantitation of cellular ribonucleotides in real biological samples have proven to be difficult using conventional techniques [21,22]. We have previously reported the application of capillary electrophoresis for the separation of purine and pyrimidine ribonucleotides [23,24]. Columns were statically coated with a mixture of polyethylene oxide, dicumylperoxide and hexamethyldisilazane (HMDS).…”

Section: Introductionmentioning

confidence: 99%

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Column wall modifications using polyethylene oxide for capillary electrophoresis of ribonucleotides

et al. 1999

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SummaryIn this study, a variety of fused silica capillaries with different combinations and sequences of treatments with HMDS and polyethylene oxide were prepared in order to develop an optimized column modification method for analysis of ribonucleotides. The 12 most common ribonucleotides (UTP, CTP, ATP, GTP, UDP, CDP, ADP, GDP, UMP, CMP, AMP, and GMP) in human cells were used as test solutes. Column performance measurements, including electroosmotic flow '(EOF), solute migration speed and retention, column efficiency, peak shape, and resolution were investigated. By analyzing solute migration speed and retention of various hydrophilic/hydrophobic solutes, the column wall effects (EOF and adsorption) can be distinguished. This analysis method can give guidance in optimizing polymer coating properties (hydrophilicity/hydrophobicity) for CE columns. By studying the performance of these columns after various surface treatments, we were able to improve the separation of ribonucleotides from real samples to within 16 minutes with high efficiency and stability (over 300 analyses) using columns first deactivated with hexamethyldisilazane, and then coated with polyethylene oxide.

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Section: Effect Of Sample-wall Interaction On Migration Speedmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Column wall modifications using polyethylene oxide for capillary electrophoresis of ribonucleotides

et al. 1999

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“…It is important to separate the analytes from the interfering background for accurate quantitation. Capillary electrophoresis (CE), whose separation is based on the different mobility of charged analytes in the presence of an external electric field, is often a suitable approach for separating the highly charged compounds such as nucleotides [16][17][18]. A number of volatile buffers such as ammonium acetate and acetic acid have been shown to provide adequate resolution for nucleotide separation [19][20][21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis‐ion trap mass spectrometry analysis of Ziagen® and its phosphorylated metabolites

Cai

Fung²,

Sinhababu

2003

Electrophoresis

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A capillary electrophoresis-ion trap mass spectrometry method with a time-segment program was developed to simultaneously analyze Ziagen and its phosphorylated metabolites such as carbovir monophosphate, carbovir diphosphate, and carbovir triphosphate. By using the time-segment program, the positively charged nucleoside analog and negatively charged nucleotides were separated and detected in a single electrophoretic run. The limits of detection were less than 2 micro M for all of the analytes. Calibration curves of the compounds showed excellent linearity over the range of 2-100 micro M. The capability of the method was demonstrated by analyzing Ziagen and its phosphorylated metabolites that were spiked in cellular extracts of human peripheral blood mononuclear cells at 20 micro M levels. Some endogenous nucleotides such as adenosine 5'-triphosphate, adenosine 5'-diphosphate, and adenosine 5'-monophosphate, were also detected in the cellular extracts.

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“…Dynamic coating is typically prepared by rinsing the capillary with a solution containing the coating material that is either a polymer or a small molecular mass compound [2]. Many polymers, such as poly(vinyl alcohol) [3], poly(alkylene glycol) [4], poly(ethylene oxide) [5], PVP [6], polyamine [7], copoly(ethylpyrrolidine methacrylate and N,N-dimethylacrylamide) [8], copoly(2-ethyl-(2-pyrrolidine)methacrylate and N,N-dimethylacrylamide) [9], and cellulose [10], have been used as dynamic coatings or physisorption materials successfully. Pallandre et al [11] have amply reviewed the modification tools of capillary surface and the main strategies for surface coating in CE and lab-onchips, and compared the advantages of physisorption, wellorganized thin self-assembled monolayers, and conversely thick polymer brushes.…”

Section: Introductionmentioning

confidence: 99%

Quaternary ammonium chitosan derivative dynamic coating for the separation of veterinary sulfonamide residues by CE with field‐amplified sample injection

Zhang

Xiao

et al. 2007

Electrophoresis

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A quaternary ammonium chitosan, 2-hydroxypropyltrimethylammonium chloride chitosan (HACC), has been developed for the dynamic coating material in CE for the first time. It presented many advantages such as favorable water solubility, satisfactory coating efficiency, and EOF toward the anode at pH >7.0. Using the modified fused-silica capillary, sulfonamides (SAs), an important group of veterinary drugs, were separated and detected by CE combined with field-amplified sample injection (FASI). The LODs of sulfonamides with UV detection were less than 0.5 ng/mL. The proposed method has been applied to the determination of veterinary sulfonamide residues in samples such as chicken, beef, and honey with fast separation (15 sulfonamides within 20 min), low LODs (0.1-0.5 ng/mL), and good reliability compared to the criteria of China (GB/T 18932.17-2003).

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Capillary Electrophoresis of Nucleotides on Ucon-Coated Fused Silica Columns

Cited by 37 publications

References 0 publications

Column wall modifications using polyethylene oxide for capillary electrophoresis of ribonucleotides

Column wall modifications using polyethylene oxide for capillary electrophoresis of ribonucleotides

Capillary electrophoresis‐ion trap mass spectrometry analysis of Ziagen® and its phosphorylated metabolites

Quaternary ammonium chitosan derivative dynamic coating for the separation of veterinary sulfonamide residues by CE with field‐amplified sample injection

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