Capillary electrophoresis of proteins 2001–2003

Hutterer, Katariina M.; Dolnı́k, Vladislav

doi:10.1002/elps.200305635

Cited by 75 publications

(42 citation statements)

References 233 publications

(166 reference statements)

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“…MS data in general are highly desirable not only in stability and purity studies [24,25] but also for revealing and monitoring diagnostic marker peptides and proteins. In capillary zone electrophoresis (CZE)-ESI-MS, mass accuracies down to 0.0008% have been realized for model mixtures of proteins [26].The high number of reviews published in the recent years, dealing not only with CE separations of proteins and peptides [27,28], but also with CE-ESI-MS coupling in general [19,20,23,29,30] …”

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confidence: 99%

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

2005

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High throughput, outstanding certainty in peptide/protein identification, exceptional resolution, and quantitative information are essential pillars in proteome research. Capillary electrophoresis (CE) coupled to mass spectrometry (MS) has proven to meet these requirements. Soft ionization techniques, such as matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI), have paved the way for the story of success of CE-MS in the analysis of biomolecules and both approaches are subject of discussion in this article. Meanwhile, CE-MS is far away from representing a homogeneous field. Therefore the review will cover a vast area including the coupling of different modes of CE (capillary zone electrophoresis, capillary isoelectric foscusing, capillary electrochromatography, micellar electrokinetic chromatography, nonaqueous capillary electrophoresis) to MS as well as on-line preconcentration techniques (transient capillary isotachophoresis, solid-phase extraction, membrane preconcentration) applied to compensate for restricted detection sensitivity. Special attention is given to improvements in interfacing, namely addressing nanospray and coaxial sheath liquid design. Peptide mapping, collision-induced dissociation with subsequent tandem MS, and amendments in mass accuracy of instruments improve information validity gained from MS data. With 2-D on-line coupling of liquid chromatography (LC) and CE a further topic will be discussed. A special section is dedicated to recent attempts in establishing CE-ESI-MS in proteomics, in the clinical and diagnostic field, and in the food sector.

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confidence: 99%

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

2005

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“…Specific reviews or books dedicated to CE analysis of peptides [4][5][6][7][8][9][10] and proteins [7][8][9][10][11][12][13][14][15] have been published, outlining that the extremely small id of the capillary has the advantage of requiring small sample volumes but often presents poor mass sensitivity. This is observed particularly with UV absorbance detection due to the short optical path length across the capillary.…”

Section: Introductionmentioning

confidence: 99%

LIF detection of peptides and proteins in CE

2007

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CE- and microchip-based separations coupled with LIF are powerful tools for the separation, detection and determination of biomolecules. CE with certain configurations has the potential to detect a small number of molecules or even a single molecule, thanks to the high spatial coherence of the laser source which permits the excitation of very small sample volumes with high efficiency. This review article discusses the use of LIF detection for the analysis of peptides and proteins in CE. The most common laser sources, basic instrumentation, derivatization modes and set-ups are briefly presented and special attention is paid to the different fluorogenic agents used for pre-, on- and postcapillary derivatization of the functional groups of these compounds. A table summarizing major applications of these derivatization reactions to the analysis of peptides and proteins in CE-LIF and a bibliography with 184 references are provided which covers papers published to the end of 2005.

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“…The increasing use of this coupled technique can be inferred by the high number of reviews published involving the use of CE-MS for proteins and peptides analysis [5][6][7][8][9][10][11][12] as well as for other biomolecules [7,[13][14][15] or more general CE-MS developments and applications [16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

2008

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In the present work, an exhaustive review of the main developments and applications of CE-MS for peptide analysis is given. This review includes the use of different CE separation modes, MS analyzers, capillary coatings, preconcentration techniques, on-chip applications as well as other different multidimensional strategies for peptide analysis. Key applications are critically discussed and relevant works published from January 2000 to May 2007 are summarized including information concerning the type of sample, CE-MS parameters as well as some figures of merit of the different CE-MS procedures developed for peptide analysis and peptidomics.

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Capillary electrophoresis of proteins 2001–2003

Cited by 75 publications

References 233 publications

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

LIF detection of peptides and proteins in CE

Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

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