Capillary Electrophoresis: Principles and Practice

Principles, Concepts and Strategies of Stereoselective Synthesis

“…In capillary electrophoresis (CE), the separation is performed in a capillary tube, and it is based on the formation of Capillary electrophoresis is one of the most efficient separation techniques available for the analysis of both large and small molecules, and it offers fast separations with exceptional efficiency. [100][101][102] Using chiral selectors as the running buffer additives or immobilized ligands, capillary electrophoresis has found a wide range of applications. CE is applied as an efficient and versatile approach for the physicochemical characterization of natural bioactive molecules, 103,104 and chiral resolution of charged substances such as biomolecules 105,106 and low-molecularweight basic or acidic drugs.…”

Precisely Controlled Smart Polymer Scaffold for Nanoscale Manipulation of Biomolecules

“…Since an uncharged molecule has zero electrophoretic mobility, the velocity, at which this specie moves from the inlet to the UV detector, corresponds to the electro-osmotic velocity of the buffer. 20 The EOF mobility was calculated as μ = (L D /t r )(L t /V), where L D is the distance from the inlet reservoir to the detector, V is the voltage difference applied across the length of the capillary (L t ), and t r is the migration time. A P/ACETM MDQ capillary electrophoresis system from Beckman Coulter Inc. was used for EOF measurements.…”

High‐sensitive protein analysis by FESI‐CE‐MALDI‐MS

“…Since the electrophoretic mobility of a protein changes with the pH of the BGE, pH is certainly the most important separation parameter as it defines the selectivity of the system [33]. As a first step, various ammonium acetate BGEs with pHs 3.5, 3.75, 4.0, 4.25, 4.5, 4.75 and 5.0 were preliminary used to determine the optimum separation pH.…”