Capillary electrophoresis separation of rosemary antioxidants from subcritical water extracts

Recent advances in the application of capillary electromigration methods for food analysis

“…These CE applications can be divided attending to the sample analyzed. Thus, grapes and wines [56][57][58][59][60][61], plants (including spices and medicinal herbs) [62][63][64][65][66][67][68], different teas [58,69,70], and olive oils [71][72][73][74] are the sources of phenols, polyphenols and pigments mainly investigated. However, some others natural samples such as berries [75], fruits [76,77], soy [78], alga and microalga [79,80] and chocolate [81] have also been analyzed by CE.…”

“…Rosemary (Rosmarinus officinalis) has been one of the most studied plants as a natural source of antioxidants (carnosic and rosmarinic acid, carnosol, etc) [66][67][68]. In this area, our group has developed different CE-UV and CE-MS procedures to analyze these polyphenols obtained from rosemary using subcritical water extraction [66,67].…”

“…In this area, our group has developed different CE-UV and CE-MS procedures to analyze these polyphenols obtained from rosemary using subcritical water extraction [66,67].…”

Analysis of natural antioxidants by capillary electromigration methods

“…Both methods were similar and provided faster analysis than HPLC, but did not allow the identification of other minor compounds present in the sample due to the inferior sensitivity of CE methods compared with HPLC. An improved method was later proposed by Crego et al [90] using a 50 mM borate buffer at pH 9.5 and 10 % acetonitrile. By using these conditions [90], it was possible to detect and separate carnosol, rosmarinic acid, carnosic acids, genkwanin and rosmanol from subcritical water rosemary extracts.…”

“…An improved method was later proposed by Crego et al [90] using a 50 mM borate buffer at pH 9.5 and 10 % acetonitrile. By using these conditions [90], it was possible to detect and separate carnosol, rosmarinic acid, carnosic acids, genkwanin and rosmanol from subcritical water rosemary extracts. Apart of CZE, MEKC has also been proposed to analyze rosemary extracts [91].…”