Capillary electrophoretic determination of amino acids improvement by cyclodextrin additives

Lee, Y.-H.; Lin, Tsung‐I

doi:10.1016/0021-9673(95)00412-g

Cited by 35 publications

(12 citation statements)

References 21 publications

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“…Owing to the formation of inclusion complexes between β-CD and a variety of guest molecules, β-CD was widely used as an additive to improve selectivity in CE [29,30]. The effect of β-CD concentration on the migration time of each amino acid was examined over a concentration range from 2 to 5 mM, using a buffer of 30 mM phosphate at pH 7.4, and an operating voltage of 20 kV.…”

Section: Optimization Of Hpce Separation Conditionsmentioning

confidence: 99%

Analysis of free amino acids during fermentation by Bacillus subtilis using capillary electrophoresis

Ren

Zhou

Zhang

et al. 2012

Biotechnol Bioproc E

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A high performance capillary electrophoresis (HPCE) method was presented to identify and quantitate free amino acids during fermentation by Bacillus subtilis. Amino acids, pre-column derivatized with phenylisothicyanate, were separated and characterized by HPCE. In order to optimize separation conditions, the assay was developed by varying the β-cyclodextrin concentration and pH of the background electrolyte. A buffer system comprising 30 mM phosphate and 3 mM β-cyclodextrin at pH 7.0, voltage of 20 kV and detection wavelength of 254 nm showed the best results, with 17 out of 20 phenylthioncarbamyl amino acids in a solution adequately separated. For quantification, p-aminobenzoic acid was added as an internal standard. Analysis of free amino acids in Bacillus subtilis culture medium using this method revealed good consistency with the values obtained using conventional ninhydrin-based amino acid analyzer. Four free amino acids (aspartic acid, glutamic acid, proline, and tyrosine) concentration in an extracellular matrix during fermentation by Bacillus subtilis were mainly monitored using this method.

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Section: Optimization Of Hpce Separation Conditionsmentioning

confidence: 99%

Analysis of free amino acids during fermentation by Bacillus subtilis using capillary electrophoresis

Ren

Zhou

Zhang

et al. 2012

Biotechnol Bioproc E

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“…Bruin et al [ 19] reported the use of salicylate as a BGE at pH 11.0 for the indirect UV detection of 7 amino acids in CZE. Lee et al [15] investigated several BGEs for CZE separation of amino acids with indirect UV detection, their results indicated that p-aminosalicylic and 4-(N,N-dimethyl amino)-benzoic acid with cyclodextrine additives [20] were the most suitable BGEs. More recently, Chen et al [21 ] reported the use of salicylate and benzoate as the BGEs for indirect UV detection of 14-18 amino acids, and its application to the determination of amino acids in urine samples.…”

Section: Introductionmentioning

confidence: 98%

Separation of amino acids in plant tissue extracts by capillary zone electrophoresis with indirect UV detection using aromatic carboxylates as background electrolytes

2000

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“…showed that the migration behavior of some amino acids in basic solution can be influenced by the addition of cyclodextrins (CDs) to the carrier electrolyte [9]. In particular, the presence of a-CD in the running buffer led to selectivity changes that increased the number of amino acids that could be separated within one single electropherogram.…”

Section: Effect Of Cyclodextrinsmentioning

confidence: 99%

Separation of native amino acids at low pH by capillary electrophoresis

Thorntion

Fritz

Klampfl

1997

J. High Resol. Chromatogr.

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SummaryAmino acids are cations at low pH and can be readily separated by capillary electrophoresis provided an alkanesulfonic acid is added to the electrolyte carrier. Formation of a positive net charge on the bare fused-silica surface at low pH was confirmed by measurement of an anodic electroosmotic flow. The addition of ethanesulfonic acid or octanesulfonic acid to the electrolyte carrier causes a reversal of the EOF. A mechanism is proposed in which the alkanesulfonic acid adsorbs to the positively-charged capillary wall through electrostatic attraction. Adsorption of a second molecule of alkanesulfonate by hydrophobic attraction to the carbon chain forms a negatively-charged coating on the capillary wall. The alkanesulfonate also imparts selectivity to the system by participation in ionpairing interactions with the native amino acids to improve resolution. The CE separation of a mixture of the twenty common amino acids at pH 2.8 with direct absorbance detection at 185 nm resulted in 17 amino acid peaks in 20 minutes with a 30 kV applied voltage. The effect of several variables was studied including electrolyte carriers containing different alkanesulfonic acids, the influence of pH, applied voltage, and concentration of electrolyte carrier.

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Capillary electrophoretic determination of amino acids improvement by cyclodextrin additives

Cited by 35 publications

References 21 publications

Analysis of free amino acids during fermentation by Bacillus subtilis using capillary electrophoresis

Analysis of free amino acids during fermentation by Bacillus subtilis using capillary electrophoresis

Separation of amino acids in plant tissue extracts by capillary zone electrophoresis with indirect UV detection using aromatic carboxylates as background electrolytes

Separation of native amino acids at low pH by capillary electrophoresis

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