Capillary gas chromatography of amino acids as their tert‐butyldimethylsilyl derivatives in the analysis of serum amino acids in metabolic diseases

Abstract

A splitless injection technique, allowing 0.5 pI injectionson 50 pm i.d. columns, has been developed.

Neurochemical analysis of amino acids, polyamines and carboxylic acids: GC–MS quantitation of tBDMS derivatives using ammonia positive chemical ionization

“…tert-Butyldimethylsilyl (tBDMS) derivatives have been utilized to monitor amino acids [1][2][3][4][5], sulfur amino acids [6,7], urea, carboxylic acids including lactate and pyruvate, ␣-keto acids, ketone bodies and free fatty acids [3,8]. This simple and robust derivatization procedure has proven extremely useful in the selected ion monitoring (SIM) quantitation of all of these compounds as well as in studies of amino acid synthesis and dynamics [9][10][11].…”

Analysis of heterocyclic aromatic amines in foods by gas chromatography–mass spectrometry as their tert.-butyldimethylsilyl derivatives

“…The mass spectra were always characterized by an intense [M-57] + ions, originated directly from the molecular ion by loss of a tert.-butyl group [C(CH 3 ) 3 ] + , and used for quantification purposes [28,30]. The [M-57] + ion is also indicative of the molecular mass.…”

“…Therefore, 1% TBDMCS is usually used as catalyst [28]. The standard or sample residues were always dried under a gentle stream of dry nitrogen and kept overnight over phosphorous pentoxide in vacuum [30] in order to prevent the presence of water. Anhydrous acetonitrile was also used as solvent in the derivatization procedure [29].…”

Isotope Dilution Analysis of Combined Nitrogen in Natural Waters. III. Nitrate and nitrite

“…The latter is facilitated by an increased solvent to reagent ratio in the sample, typical of carboxylic acids and phenols (9:1), as opposed to 1:1 commonly used for amine and amino acid analysis. 27 This reduces ion source contamination by silylation reagents and therefore reduces maintenance down-time. The superiority of the TBDMS derivative over the trimethylsilyl derivative used in our ammonium method 4 has caused us to introduce this derivative for the latter method also.…”