“…Surface modification can be essential to the operation of a microfluidic channel. Many surface modification techniques have been developed, including plasma pretreatment and silanization, chemical vapor deposition, UV irradiation, photochemical grafting, nanomaterial deposition, and atomic layer deposition, among others. , Some key methods of surface preparation require exposure to an environmental treatment, such as plasma treatment to render the surface wettable ,,,, or chemical vapor deposition to protect the material from solvents ,,,, (Figure B). In closed systems, the surface modification may be performed before bonding, in which case it may affect the bonding efficiency or be affected by the bonding process (e.g., temperature or solvents) .…”