Caprin-1 is a novel microRNA-223 target for regulating the proliferation and invasion of human breast cancer cells

Gong, Bo; Hu, Heyu; Chen, Jia; Cao, Shuang; Yu, Jing; Xue, Jianxiang; Chen, Fuhua; Cai, Ye; He, Hong; Zhang, Lei

doi:10.1016/j.biopha.2013.06.006

Cited by 62 publications

(64 citation statements)

References 26 publications

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“…In our study, miR-223 was identified as a regulator of STIM1 via qRT-PCR, western blot analysis and luciferase reporter assay. Additionally, HAX-1 [27] and Caprin-1 [22] have also been identified as the target genes of miR-223 in breast cancer.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have shown that miRNAs act as a means of anticancer and play an important role in regulating the invasion and migration of cancer [16-18]. Notably, recent studies revealed that a variety of miRNAs, including miR-152 [19], miR-381 [20], miR-211 [21], miR-223 [22] and so on, could inhibit tumor proliferation, invasion and metastasis in breast cancer by targeting various genes. However, the miRNAs targeting STIM1 in breast cancer are still poorly characterized.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast Cancer

Yang¹,

Jiang

Ma³

et al. 2018

Cell Physiol Biochem

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Background/Aims: To investigate the cellular effects and clinical significance of microRNA-223 (miR-223) in breast cancer by targeting stromal interaction molecule1 (STIM1). Methods: Breast cancer cell lines (T47D, MCF-7, SKB-R3, MDA-MB-231 and MDA-MB-435) and a normal breast epithelial cell line (MCF-10A) were prepared for this study. MiR-223 mimics, anti-miR-223 and pcDNA 3.1-STIM1 were transiently transfected into cancer cells independently or together, and then RT-qPCR was performed to detect the expressions of miR-223 and STIM1 mRNA, dual-luciferase reporter assay was conducted to examine the effects of miR-223 on STIM1, Western blotting was used to measure the expressions of the STIM1 proteins, MTT and Trans-well assays were performed to detect cell proliferation and invasion. Finally, the correlation of miR-223 and STIM1 was investigated by detecting with ISH and IHC in breast cancer specimens or the corresponding adjacent normal tissues. Results: Compared with normal cells and tissues, breast cancer tissues and cells exhibited significantly lower expression of miR-223, but higher expression of STIM1. MiR-223 could inhibit the proliferation and invasiveness of breast cancer cells by negatively regulating the expressions of STIM1. Reimplantation with STIM1 partially rescued the miRNA-223-induced inhibition of breast cancer cells. Clinical data revealed that high expression of STIM1 and miR-223 was respectively detrimental and beneficial factor impacting patient’s disease-free survival (DFS) rather than overall survival (OS). Moreover, Pearson correlation analysis also confirmed that STIM1 was inversely correlated with miR-223. Conclusion: MiR-223 inhibits the proliferation and invasion of breast cancer by targeting STIM1. The miR-223/STIM1 axis could possibly be a potential therapeutic target for treating breast cancer patients.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast Cancer

Yang¹,

Jiang

Ma³

et al. 2018

Cell Physiol Biochem

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“…They control the expression of target genes by binding to the 3'-untranslated region (UTR) of their associated mRNAs and serve an important role in a variety of biological processes, including cell proliferation, apoptosis, differentiation, invasion and migration (5)(6)(7)(8). Accumulating studies are demonstrating that miRNAs are dysregulated in a variety of cancers and serve a critical role in tumorigenesis (9)(10)(11)(12)(13)(14). Recent studies demonstrated that miRNAs are critical regulators in the development and progression of cancer, including cervical cancer (15,16).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of miR‑30a is associated with proliferation and invasion via targeting MEF2D in cervical cancer

Zhao

Shu

et al. 2017

Oncol Lett

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Abstract. Accumulating studies have revealed that microRNAs serve crucial roles in cancer development and progression. MicroRNA-30a (miR-30a) has been implicated in various cancer types. However, the role of miR-30a in cervical cancer remains unclear. In the current study, a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay revealed that miR-30a was significantly downregulated in cervical cancer tissues compared with adjacent normal tissues, and in the cervical cancer cell lines HeLa, SiHa and Ca-Ski compared with GH329 normal cervical epithelial cells. A functional assay using miR-30a mimic demonstrated that miR-30a could inhibit the growth and invasion of cervical cancer cells. Additionally, bioinformatics-based prediction and luciferase reporter assays indicated that MEF2D is a direct target of miR-30a. Transfection with miR-30a reduced the mRNA expression and protein levels of MEF2D, as determined using RT-qPCR and western blot analyses. Furthermore, MEF2D expression was negatively correlated with that of miR-30a in cervical cancers. Overall, the present study demonstrated that miR-30a functions as a tumor suppressor by targeting MEF2D in cervical cancer, which may provide the basis for a prognostic biomarker or therapeutic strategy for cervical cancer.

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“…Caprin-1 mRNA is a target of the microRNAs miR-223 and miR-16 (Kaddar et al, 2009;Gong et al, 2013). In a study on the expression of Caprin-1 and miR-223 in several breast cancer cells and normal breast cells, miR-223 expression levels were significantly lower in cancer cells than in normal cells, while Caprin-1 expression was higher in cancer cells than in normal cells (Gong et al, 2013). Overexpression of Caprin-1 promoted the proliferation and invasion of breast cancer cells, which was inhibited by miR-223.…”

Section: Introductionmentioning

confidence: 99%

Bacterial expression and preliminary crystallographic studies of a 149-residue fragment of human Caprin-1

Zhu

Huang

et al. 2015

Acta Cryst Sect F

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Caprin-1 is an RNA-binding protein which plays critical roles in several important biological processes, including cellular proliferation, the interferonmediated antiviral innate immune response, the maintenance of synaptic plasticity and the formation of RNA stress granules. Caprin-1 has been implicated in the pathogenesis of several human diseases, including osteosarcoma, breast cancer, viral infections, hearing loss and neurodegenerative disorders. Despite the emerging biological and physiopathological significance of Caprin-1, no structural information is available for this protein. Moreover, Caprin-1 does not have sequence similarity to any other protein with a known structure. It is therefore expected that structural studies will play a particularly crucial role in revealing the functional mechanisms of Caprin-1. Here, a protein fragment of human Caprin-1 consisting of residues 112-260 was expressed, purified and crystallized. Native and Se-SAD data sets were collected to resolutions to 2.05 and 2.65 Å , respectively, in different space groups.

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Caprin-1 is a novel microRNA-223 target for regulating the proliferation and invasion of human breast cancer cells

Cited by 62 publications

References 26 publications

MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast Cancer

MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast Cancer

Downregulation of miR‑30a is associated with proliferation and invasion via targeting MEF2D in cervical cancer

Bacterial expression and preliminary crystallographic studies of a 149-residue fragment of human Caprin-1

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