Capsaicin sensitizes TRAIL-induced apoptosis through Sp1-mediated DR5 up-regulation: Involvement of Ca2+ influx

Moon, Dong‐Oh; Kang, Chang‐Hee; Kang, Sang-Hyuck; Choi, Yung Hyun; Hyun, Jin‐Won; Chang, Weon-Young; Kang, Hee‐Kyoung; Koh, Young‐Sang; Maeng, Young-Hee; Kim, Young-Ree; Kim, Gi‐Young

doi:10.1016/j.taap.2011.12.010

Cited by 45 publications

(30 citation statements)

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“…DDP (sub-toxic dose) also has little influence on the activity of the cells. However, the combination of TRAIL and DDP could inhibit the survival rate and significantly increase the apoptosis rate of SGC7901/VCR cells, and it shows a synergy effect, which is consistent with the conclusions of previous studies [18, 19]. In the present study, we found that either TRAIL or the combination of TRAIL and DDP could inhibit the expression of MDR1 and P-gp in SGC7901/VCR cells, and when compared with the TRAIL group, the difference is significant.…”

Section: Discussionsupporting

confidence: 92%

Effect of TRAIL in combination with DDP on the expression of MDR1 gene in gastric cancer cells

Cui

Wang

et al. 2014

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IntroductionGastric cancer is one of the most common malignant tumor, and gastric cancer is the second most common cause of cancer mortality worldwide. Although chemotherapy is one of the most important treatment options for gastric cancer, and could improve the overall survival rate and quality of live, one significant reason for its failure is multidrug resistance (MDR).AimTo study the effect of tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) combined with chemotherapeutic drug cisplatin (DDP) on the expression of multidrug resistance gene 1 (MDR1) in the gastric cancer cell line SGC-7901/VCR.Material and methodsSGC-7901/VCR cells were cultured with DDP and TRAIL in various concentrations. The apoptosis rate was separately measured by a flow cytometer in DDP (sub-toxic dose) alone, TRAIL (200 µg/l) alone and in a combination of the two. Expression levels of MDR1 mRNA and P-glycoprotein (P-gp) were detected by RT-PCR and ELISA analysis, respectively.ResultsThe apoptosis rate in the combination group was significantly higher than that in the other groups (p < 0.05). According to the results of RT-PCR and ELISA, the expressions of MDR1 mRNA and P-gp in the combination group were statistically significant different compared with other groups (p < 0.05).ConclusionsThe combination of TRAIL with DDP could reverse MDR phenotype in gastric cancer cell line SGC7901/VCR. The mechanism may be involved in the down-regulation of MDR1 mRNA and P-gp, which may play an essential role in overcoming the chemotherapeutic resistance of gastric cancer cells. This study indicates that a combination of chemotherapy and TRAIL may be an effective strategy to treat MDR gastric cancer.

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Section: Discussionsupporting

confidence: 92%

Effect of TRAIL in combination with DDP on the expression of MDR1 gene in gastric cancer cells

Cui

Wang

et al. 2014

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“…In the same cells, capsaicin increased ROS production and induced expression of heme oxygenase-1 (HO-1) (Lee et al 2004;Joung et al 2007). Likewise, capsaicin sensitized hepatocellular carcinoma cells as well as breast cancer cells and leukemia cells to apoptosis induced by tumor necrosis factor-related, apoptosis-inducing ligand (TRAIL) (Moon et al 2012). Co-treatment with capsaicin and TRAIL potentiated the caspase 3, 8, and 9 activation, the release of cytochrome C, and the disruption of mitochondrial transmembrane potential.…”

Section: Hepatocellular Carcinomamentioning

confidence: 99%

The Potential Antitumor Effects of Capsaicin

Díaz‐Laviada

Rodríguez-Henche

2014

Capsaicin as a Therapeutic Molecule

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Capsaicin, one of the major pungent ingredients found in red peppers, has been recently demonstrated to induce apoptosis in many types of malignant cell lines including colon adenocarcinoma, pancreatic cancer, hepatocellular carcinoma, prostate cancer, breast cancer, and many others. The mechanism whereby capsaicin induces apoptosis in cancer cells is not completely elucidated but involves intracellular calcium increase, reactive oxygen species generation, disruption of mitochondrial membrane transition potential, and activation of transcription factors such as NFκB and STATS. Recently, a role for the AMP-dependent kinase (AMPK) and autophagy pathways in capsaicin-triggered cell death has been proposed. In addition, capsaicin shows antitumor activity in vivo by reducing the growth of many tumors induced in mice. In this chapter, we report the last advances performed in the antitumor activity of capsaicin and review the main signaling pathways involved. CapsaicinCapsaicin (8-methyl-N-vanillyl-6-noneamide) is the major constituent of the peppers belonging to the genus Capsicum and responsible for their spicy flavor and burning sensation, also known as pungency. Besides capsaicin, a family of related compounds are present in Capsicum sp. plants, all referred as capsaicinoids. These compounds are capsaicin, dihydrocapsaicin, nordihydrocapsaicin, and homocapsaicin. Capsaicin

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“…Alpha glucosidase and α-amylase inhibitory activities of capsicum was evaluated by scientists 26 It induced autophagy, induced anti-tumorigenesis, deregulation of B-catechin, and TCF dependent signaling [32][33] . Anti-cancer activity of capsaicin on human breast cancer was studied on MCF-7, T47D, BT-474, SKBR-3 and MDA-MB231 cell lines where it causes mitochondrial dysfunction 34 , increased apoptosis and arrest cell cycle into G2/M phase [35][36][37][38] . Human myeloid leukemia also recovered by capsicum and studied on HL-60, U937 and THP-1 cell lines where it enhances the apoptotic effects by activating the calciumCaMKII-Sp1 pathway 38 .…”

Section: Anti-diabetic Activitymentioning

confidence: 99%

“…Anti-cancer activity of capsaicin on human breast cancer was studied on MCF-7, T47D, BT-474, SKBR-3 and MDA-MB231 cell lines where it causes mitochondrial dysfunction 34 , increased apoptosis and arrest cell cycle into G2/M phase [35][36][37][38] . Human myeloid leukemia also recovered by capsicum and studied on HL-60, U937 and THP-1 cell lines where it enhances the apoptotic effects by activating the calciumCaMKII-Sp1 pathway 38 .…”

Section: Anti-diabetic Activitymentioning

confidence: 99%

“…Capsicum inhibit human hepatoma on HepG2 cell line by decreasing cell viability, generated ROS, activated caspase-3, induced apoptosis and autophagy 42 . It also enhances apoptotic effects by activating the calciumCaMKII-Sp1 pathway on Hep3B cell lines 38 . Human nasopharyngeal carcinoma was recovered by capsicum studied on NPC-TW 039 cell lines by inducing G0/G1 phase arrest, apoptosis and increased cytosolic Ca2+ level 43 .…”

Section: Anti-diabetic Activitymentioning

confidence: 99%

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2017

IJPSR

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Nature always stands as a golden mark to exemplify the outstanding phenomena of symbiosis. Natural products from plant, animal and minerals have been the basis of the treatment of human disease. Capsicum plants belongs to the family Solanaceae and kingdom Plantae, consist of 27 species, and approximately 3000 varieties. Chili peppers are used worldwide in foods for their pungent flavor, aroma, and to prolong food spoilage. With capsaicin contents ranging from zero to millions of Scoville heat units, the different varieties offer a wide range of options for people all over the world. The aim of present review is to form a short compilation of the pharmacological and toxicological potentials of this multipurpose fruit. Capsicum, has been used as anti-inflammatory, anti-diabetic, anti-cancer, anti-ulcer, anticoagulant, analgesic, anti-arthritis, immuno-modulatory, memory enhancing, pain relief, hypocholesterolaemic, hypolipidemic, hepatoprotective and antimicrobial effects. It also cause irritation to mucous membrane, inflammation and neurotoxicity. It should not be used on open wounds or abrasions, or near the eyes. It has antagonistic effects on of α-adrenergic blockers, clonidine and methyldopa.

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Capsaicin sensitizes TRAIL-induced apoptosis through Sp1-mediated DR5 up-regulation: Involvement of Ca2+ influx

Cited by 45 publications

References 34 publications

Effect of TRAIL in combination with DDP on the expression of MDR1 gene in gastric cancer cells

Effect of TRAIL in combination with DDP on the expression of MDR1 gene in gastric cancer cells

The Potential Antitumor Effects of Capsaicin

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