Capsicum annuum L. var. Cornetto di Pontecorvo PDO: Polyphenolic profile and in vitro biological activities

Sotto, Antonella Di; Vecchiato, Martina; Abete, Lorena; Toniolo, Chiara; Giusti, Anna Maria; Mannina, Luisa; Locatelli, Marcello; Nicoletti, Marcello; Giacomo, Silvia Di

doi:10.1016/j.jff.2017.11.041

Cited by 37 publications

(39 citation statements)

References 92 publications

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“…Following our previous research on plant materials used as tea (Melucci et al, 2013), foods (Sobolev et al, 2018;Di Sotto et al, 2018), food supplements , traditional herbal medicine , and their content of toxic/heavy metals (Locatelli et al, 2014;Melucci et al, 2018), we highlight and complete here the phytochemical information on this traditionally used medicinal plant.…”

Section: Introductionmentioning

confidence: 99%

Phenolic Profile and Bioactivities of Sideritis perfoliata L.: The Plant, Its Most Active Extract, and Its Broad Biological Properties

et al. 2020

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Sideritis, also named "ironwort," "mountain tea," or "shepherd's tea," is a genus of flowering plants used as herbal medicine in traditional Mediterranean-area medicine systems, and these plants are generally consumed as a herbal tea. Its use as herbal tea and in traditional herbal medicine is quite popular. There are currently few studies on Sideritis perfoliata L., and only one reports the use of a liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry (LC-DAD-ESI-MS n ) profile and the content of phenolic compounds without considering a possible correlation with its biological activities. This paper aims to investigate the antioxidant activities by means of several different biological/biochemical assays (radical scavenging, reducing power, ferrous ion chelating, and total antioxidant by phosphomolybdenum and b-carotene bleaching methods) as well as analyze the enzyme inhibitory activities (against AChE (acetylcholinesterase), BChE (butyrylcholinesterase), tyrosinase, a-glucosidase, and a-amylase) as well as the total phenolics, flavonoids, and condensed tannins. The reported results on Sideritis perfoliata highlighted that methanol and water extracts generally showed higher radical scavenging and reducing power activities. A similar trend could be observed for phosphomolybdenum and ferrous ion chelating activities. Methanol extracts showed lower activity only for the b-carotene bleaching assay.

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Section: Introductionmentioning

confidence: 99%

Phenolic Profile and Bioactivities of Sideritis perfoliata L.: The Plant, Its Most Active Extract, and Its Broad Biological Properties

et al. 2020

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“…The α-amylase activity was determined by the method of dinitrosalicilic acid (DNSA), according to Di Sotto et al [71], with minor changes. To perform the assay, serial dilutions of the extract (250 μl; 1-100 μg/mL final concentration, 1:2 to 1:5 dilution factor) were pre-incubated with α-amylase (250 μL; 0.5 mg/mL in phosphate buffer solution, corresponding to 25 U/mL) for 10 minutes at 37 °C.…”

Section: α-Amylase Inhibitionmentioning

confidence: 99%

Hypoglycemic, Antiglycation and Cytoprotective Properties of A Phenol Rich Extract from Waste Peel of Punica Granatum L. Var. Dente Di Cavallo DC2

Sotto¹,

Locatelli²,

Macone³

et al. 2019

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Pomegranate peel is a natural source of phenolics, claimed to possess healing properties, among which antioxidant and antidiabetic. In line with this evidence, the ethyl acetate PGE extract, obtained by Soxhlet from the peel of Dente di cavallo DC2 variety and characterized by a 4% amount of ellagic acid, has been studied for its hypoglycemic, antiglycation and antioxidative cytoprotective properties, in order to support a possible further nutraceutical interest. The α-amylase and α-glucosidase enzyme inhibition, interference with advanced glycation end-products (AGE) formation and metal chelating abilities were evaluated as hypoglycemic mechanisms. Also, considering that oxidative stress is associated with hyperglycemia complications, PGE antioxidant cytoprotective properties under hyperglycemic conditions were assayed. Phenolic profile was characterized by integrated chromatographic and spectrophotometric methods. Under our experimental conditions, PGE strongly inhibited the tested enzymes, especially α-glucosidase, and exerted chelating and antiglycation properties. Also, it reduced both ROS and GSH levels under hyperglycemic conditions, thus suggesting its ability to support cell functions by counteracting intracellular oxidative stress. Along with ellagic acid, rutin was the major identified flavonoid (about 4 %) of PGE. Present results suggest PGE to be a possible remedy for hyperglycemia management and encourage further studies to exploit its promising properties.

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“…According to the Pearson analysis, the scavenger activities against DPPH and ABTS radicals were significantly correlated for both DR2B and DR2C extracts (Tables 5 and 6), although they exhibited higher potency against ABTS respect to DPPH radical (2.6 and 3.4 folds respectively). DPPH is a pre-existing radical, which can be neutralized by electron or hydrogen transfer, thus forming a stable diamagnetic molecule; conversely, ABTS cation, generated just before the experiments through different activators, requires an electron-transfer process to be scavenged and is more reactive than DPPH [62]. These important chemical features can lead to different affinities and kinetics reactions and in the scavenging potencies.…”

Section: Antioxidant Activity Assaysmentioning

confidence: 99%

“…The total polyphenol and flavonoid content was determined according to Di Sotto et al [62]. The total polyphenol content was calculated as chlorogenic acid equivalents (CAE) per milligram of sample, while total flavonoids were expressed as QE per milligram of sample.…”

Section: Determination Of Total Polyphenol and Flavonoid Contentmentioning

confidence: 99%

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A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex virus Type 1 Activity In Vitro

Sotto

Giacomo

Amatore

et al. 2018

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DR2B and DR2C extracts, from peel of commercially and physiologically ripe eggplants, were studied for the antioxidative cytoprotective properties and anti-HSV-1 activity, in line with the evidence that several antioxidants can impair viral replication by maintaining reducing conditions into the host cells. The antioxidative cytoprotective effects against tBOOH-induced damage was assessed in Caco2 cells, while the antiviral activity was studied in Vero cells; phenolic and anthocyanin fingerprint was characterized by integrated phytochemical methods. Results highlighted different compositions of the extracts, with chlorogenic acid and delphinidin-3-rutinoside as the major constituents; other peculiar phytochemicals were also identified. DR2C resulted able to partly counteract the tBOOH-induced cytotoxicity, with a remarkable lowering of lactate metabolism under both normoxia and hypoxia. DR2B and DR2C reduced ROS production, possessed scavenging and chelating properties. Interestingly, DR2C increased intracellular GSH levels. Furthermore, DR2Cinhibited the HSV-1 replication when added for 24 h after viral adsorption, as also confirmed by the reduction of many viral proteins expression. Since DR2C was able to reduce NOX4 expression during HSV-1 infection, its antiviral activity may be correlated to its antioxidant properties. Although further studies are needed to better characterize DR2C activity, the results suggest this extract as a promising new anti-HSV-1 agent.

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Capsicum annuum L. var. Cornetto di Pontecorvo PDO: Polyphenolic profile and in vitro biological activities

Cited by 37 publications

References 92 publications

Phenolic Profile and Bioactivities of Sideritis perfoliata L.: The Plant, Its Most Active Extract, and Its Broad Biological Properties

Phenolic Profile and Bioactivities of Sideritis perfoliata L.: The Plant, Its Most Active Extract, and Its Broad Biological Properties

Hypoglycemic, Antiglycation and Cytoprotective Properties of A Phenol Rich Extract from Waste Peel of <i>Punica Granatum</i> L. Var. Dente Di Cavallo DC2

A Polyphenol Rich Extract from <em>Solanum melongena</em> L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex virus Type 1 Activity <em>In Vitro</em>

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