Capsular and extracellular polysaccharides of the diazotrophic rhizobacterium Herbaspirillum seropedicae Z78

Smol’kina, O. N.; Shishonkova, N. S.; Yurasov, Nikolay A.; Игнатов, В. В.

doi:10.1134/s0026261712030113

Cited by 13 publications

(10 citation statements)

References 25 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analyses of H. seropedicae eps genes that code for glycosyltransferases and sugar modifying proteins (such as epimerases and phosphatases in Table S1 ) suggest that the EPS is composed of these same monosaccharides. Indeed, monosaccharide composition analysis of H. seropedicae Z67 T EPS showed galactose, glucose and mannose as constituents at a proportion of 4∶3:1, with possible substitutions with tetracarboxylic acids [53] .…”

Section: Resultsmentioning

confidence: 99%

Exopolysaccharide Biosynthesis Enables Mature Biofilm Formation on Abiotic Surfaces by Herbaspirillum seropedicae

et al. 2014

View full text Add to dashboard Cite

H. seropedicae associates endophytically and epiphytically with important poaceous crops and is capable of promoting their growth. The molecular mechanisms involved in plant colonization by this microrganism are not fully understood. Exopolysaccharides (EPS) are usually necessary for bacterial attachment to solid surfaces, to other bacteria, and to form biofilms. The role of H. seropedicae SmR1 exopolysaccharide in biofilm formation on both inert and plant substrates was assessed by characterization of a mutant in the espB gene which codes for a glucosyltransferase. The mutant strain was severely affected in EPS production and biofilm formation on glass wool. In contrast, the plant colonization capacity of the mutant strain was not altered when compared to the parental strain. The requirement of EPS for biofilm formation on inert surface was reinforced by the induction of eps genes in biofilms grown on glass and polypropylene. On the other hand, a strong repression of eps genes was observed in H. seropedicae cells adhered to maize roots. Our data suggest that H. seropedicae EPS is a structural component of mature biofilms, but this development stage of biofilm is not achieved during plant colonization.

show abstract

Section: Resultsmentioning

confidence: 99%

Exopolysaccharide Biosynthesis Enables Mature Biofilm Formation on Abiotic Surfaces by Herbaspirillum seropedicae

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Despite the presence of KDO and~40% of hydroxylated fatty acids, the EPS is a polysaccharide-lipid complex. This assumption is based on the fact that the ultracentrifugation of the EPS did not yield a transparent gel-like sediment (LPS), contrary to what was observed by Smol'kina et al (2012).…”

Section: Discussionmentioning

confidence: 88%

Characterization of biopolymers produced by planktonic and biofilm cells ofHerbaspirillum lusitanumP6‐12

Velichko

Grinev

Fedonenko

2020

J. Appl. Microbiol.

View full text Add to dashboard Cite

Aims: The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile. Methods and Results: Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry. A high degree of polymer separation and purification was achieved by ultracentrifugation, and column chromatography allowed us to identify the chemical differences between biopolymers from biofilm and planktonic H. lusitanum. It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS). The EPS, CPS-I, CPS-II had different monosaccharide and lipid compositions. The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%). From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%). The cells outside the biofilm had capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPS FBC) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%). Conclusions: During biofilm formation, the bacteria secreted surface biopolymers that differed from those of the planktonic cells. The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues. Significance and Impact of the Study: The results of the study permit a better understanding of the physiological properties of the biopolymers, for example, in plant-microbe interactions.

show abstract

“…Herbaspirillum seropedicae Z78 (IBPPM 217) was from the IBPPM RAS Collection of Rhizosphere Microorganisms (http://collection.ibppm.ru). Cells were grown in a vitaminsupplemented liquid synthetic nutrient medium (Smol'kina et al 2012) at 30 ± 1°C for 24 h (until the end of the exponential growth phase).…”

Section: Strain and Growth Conditionsmentioning

confidence: 99%

“…The cells were then degreased with petroleum ether, dried with acetone, and finely dispersed. CPS-I, CPS-II, EPS-I and EPS-II were isolated as described by Smol'kina et al (Smol'kina et al 2012). All carbohydrate-containing fractions that did not give absorption between 240 and 260 nm were pooled, concentrated, and lyophilized.…”

Section: Isolation and Purification Of Bacterial Polysaccharidesmentioning

confidence: 99%

See 1 more Smart Citation

Phage antibodies for the immunochemical characterization of Herbaspirillum seropedicae Z78 glycopolymers

Velichko

Fedonenko

2019

Ann Microbiol

View full text Add to dashboard Cite

Purpose Microbial carbohydrate antigens are targets of the immune systems of hosts. In this context, it is of interest to obtain data that will permit judgment of the degree of heterogeneity, chemical makeup, and localization of the antigenic determinants of the Herbaspirillum surface glycopolymers. Methods A sheep single-chain antibody-fragment phage library (Griffin.1, UK) was used to obtain miniantibodies to the exopolysaccharides (EPS-I and EPS-II), capsular polysaccharides (CPS-I and CPS-II) and lipopolysaccharide (LPS) of Herbaspirillum seropedicae Z78. To infer about the presence or absence of common antigenic determinants in the cell-surface polysaccharides of H. seropedicae Z78, we ran a comparative immunoassay using rabbit polyclonal and phage recombinant antibodies to the surface glycopolymers of H. seropedicae Z78. Results We isolated and purified the exopolysaccharides (EPS-I and EPS-II), capsular polysaccharides (CPS-I and CPS-II), and lipopolysaccharide (LPS) of Herbaspirillum seropedicae Z78. Using rabbit polyclonal antibodies, we found that these cellsurface polysaccharides were of a complex nature. EPS-I, EPS-II, CPS-I, CPS-II, and LPS contained common antigenic determinants. CPS-I, CPS-II, and LPS also contained individual antigenic determinants composed of rhamnose, N-acetyl-D-glucosamine, and N-acetyl-D-galactosamine-sugars responsible for cross-reactions with miniantibodies. Conclusions The anti-LPS miniantibodies were more specific for the core region of the LPS, in which rhamnose was the most abundant sugar, than they were specific for its O portion. The miniantibodies we isolated can be useful reagents not only in basic biochemical research but also in clinical diagnostic and therapeutic applications.

show abstract

Capsular and extracellular polysaccharides of the diazotrophic rhizobacterium Herbaspirillum seropedicae Z78

Cited by 13 publications

References 25 publications

Exopolysaccharide Biosynthesis Enables Mature Biofilm Formation on Abiotic Surfaces by Herbaspirillum seropedicae

Exopolysaccharide Biosynthesis Enables Mature Biofilm Formation on Abiotic Surfaces by Herbaspirillum seropedicae

Characterization of biopolymers produced by planktonic and biofilm cells ofHerbaspirillum lusitanumP6‐12

Phage antibodies for the immunochemical characterization of Herbaspirillum seropedicae Z78 glycopolymers

Contact Info

Product

Resources

About