Capsule thickness and amounts of a 39kDa capsular protein of avian Pasteurella multocida type A strains correlate with their pathogenicity for chickens

Borrathybay, Entomack; Sawada, Takuo; Kataoka, Yuki; Okiyama, Emi; Kawamoto, Eiichi; Amao, Hiromi

doi:10.1016/j.vetmic.2003.09.011

Cited by 28 publications

(33 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of the capsule mainly increases the resistance of a P. multocida cell to phagocytosis and enhances its virulence potency when compared with non-capsulated strains (Maheswaran and Thies, 1979;Borrathybay et al, 2003). The fact that P. multocida is a commonly encapsulated organism is supported by this study, too.…”

Section: Discussionsupporting

confidence: 62%

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Jaglič¹,

Kučerová²,

Nedbalcová³

et al. 2006

Vet. Med. - Czech

View full text Add to dashboard Cite

Twenty seven Pasteurella multocida rabbit isolates were characterised by pulsed-field gel electrophoresis (PFGE) generated by restriction endonuclease ApaI, and examined for the presence of capsule and fimbriae, and tested of haemolytic activity and endotoxin release in presence of rabbit plasma. Among the isolates a high number (n = 20) of different PFGE types was observed. All isolates were found to be encapsulated and four of them also possessed fimbriae. Although the presence of the aphA gene, conferring a haemolytic phenotype for Escherichia coli under anaerobic conditions, was indicated in all of the isolates by PCR, clear haemolytic activity was observed in only one isolate when grown anaerobically. All of the isolates grew easily in the presence of rabbit plasma which showed a high capacity to bind the released endotoxin.

show abstract

Section: Discussionsupporting

confidence: 62%

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Jaglič¹,

Kučerová²,

Nedbalcová³

et al. 2006

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…Since that time, the outbreaks in various hosts have been reported. Capsular serogroup A and somatic serotype 3 (A:3) was predominated serovar and the some of these isolates produced the lower amount of capsule material [4,20]. A non-capsulated strain of P. multocida was obtained from strain P-1059 (serovar A:3) by 35-serial passages on DSA plates and designated strain P-1059B [4].…”

Section: Discussionmentioning

confidence: 99%

“…Capsular serogroup A and somatic serotype 3 (A:3) was predominated serovar and the some of these isolates produced the lower amount of capsule material [4,20]. A non-capsulated strain of P. multocida was obtained from strain P-1059 (serovar A:3) by 35-serial passages on DSA plates and designated strain P-1059B [4]. This variant strain produced bluish colony on DSA observed by the obliquely-transmitted light stereomicroscope and its capsule thickness also significantly related to its pathogenicity for chickens (Borrathybay et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…A non-capsulated strain of P. multocida was obtained from strain P-1059 (serovar A:3) by 35-serial passages on DSA plates and designated strain P-1059B [4]. This variant strain produced bluish colony on DSA observed by the obliquely-transmitted light stereomicroscope and its capsule thickness also significantly related to its pathogenicity for chickens (Borrathybay et al, 2003). Our preliminary investigation on avian pasteurellosis had identified the gene coding of the adhesive protein (cp39) of the avian isolates and the PCR-RFLP employed EcoRI or HindIII was performed [21].…”

Section: Discussionmentioning

confidence: 99%

“…In Japan, strain of P. multocida capsular serogroup A and somatic serotype 3 was predominated among avian isolates and strains of somatic serotypes 1, 4 and 10 were also isolated [20]. Some of the clinical isolates were determined for their capsulation and virulence for chicken by the observation of their colonies by electron microscope or obliquely-transmitted light stereomicroscope and experimental infection in chickens [4]. Our previous investigations indicated the genetic variation of the adhesive protein gene among each isolates by PCR-RFLP employing the HindIII and EcoRI digestion [21].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Molecular Epidemiology of Japanese Avian Pasteurella multocida Strains by the Single-Enzyme Amplified Fragment Length Polymorphism and Pulsed-Field Gel Electrophoresis

Sthitmatee

Kataoka

Sawada

2010

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Molecular epidemiology analyses of the 36 clinical isolates of Pasteurella multocida from various avian hosts in Japan between 1976 to 2007 including 5 reference strains from the U.S.A., Taiwan and Indonesia were performed by employing the single-enzyme amplified fragment length polymorphism (SE-AFLP) comparison with the classical ApaI-based pulsed-field gel electrophoresis (PFGE). As the results, SE-AFLP gave 21 profiles while PFGE gave 20 profiles. The Simpson's index of diversity analysis indicated that SE-AFLP gave a high discrimination power than PFGE. This concluded that SE-AFLP is a higher discrimination power than PFGE to differentiate avian P. multocida isolates in Japan. In addition, the genetical profiles suggested that there is the evolution of somatic serotype 3 strain in the indigenous host of Japan.KEY WORDS: discrimination power, Pasteurella multocida, PFGE, SE-AFLP.J. Vet. Med. Sci. 72(11): 1465-1470, 2010 Fowl cholera is an important disease in poultry industry around the world. Pasteurella multocida of the gram negative bacteria is recognized as the etiologic agent of fowl cholera [16,18]. The bacterium can be classified by capsular serogrouping into 5 serogroups (A, B, D, E and F) [6,17] and by lipopolysaccharide into 16 somatic serotypes (1-16) [5]. Fowl cholera usually appears a septicemic disease in the acute or peracute conditions associated with the high mortality and morbidity but the chronic conditions are also observed [16,18]. In Japan, strain of P. multocida capsular serogroup A and somatic serotype 3 was predominated among avian isolates and strains of somatic serotypes 1, 4 and 10 were also isolated [20]. Some of the clinical isolates were determined for their capsulation and virulence for chicken by the observation of their colonies by electron microscope or obliquely-transmitted light stereomicroscope and experimental infection in chickens [4]. Our previous investigations indicated the genetic variation of the adhesive protein gene among each isolates by PCR-RFLP employing the HindIII and EcoRI digestion [21]. However, the genetic epidemiology of these isolates needs to be clarified.Since a number of DNA-based typing systems have been employed to characterize the isolates [1,3,10,14,15]. Pulsed-field gel electrophoresis (PFGE) is recognized as the classical DNA-based typing method of the isolates. However, the higher discrimination power DNA-based typing methods have been demonstrated. Previous studies suggested that the amplified fragment length polymorphism (AFLP) have been recognized as the typing method that showed the higher power of discrimination than either PFGE or the random amplified polymorphism DNA (RAPD) [1,8]. However, the DNA-based typing method requires more knowledge about DNA to analyze [8]. Then, the recent study employed the SE-AFLP and PFGE to differentiate avian P. multocida isolates in Japan and the discriminatory power of the typing methods was also compared. MATERIALS AND METHODSBacterial strains and growth condition: P. multocida strains were ...

show abstract

Avian Cholera

Samuel¹,

Botzler²,

Wobeser³

2007

Infectious Diseases of Wild Birds

View full text Add to dashboard Cite

Capsule thickness and amounts of a 39kDa capsular protein of avian Pasteurella multocida type A strains correlate with their pathogenicity for chickens

Cited by 28 publications

References 23 publications

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Molecular Epidemiology of Japanese Avian Pasteurella multocida Strains by the Single-Enzyme Amplified Fragment Length Polymorphism and Pulsed-Field Gel Electrophoresis

Avian Cholera

Contact Info

Product

Resources

About