Captavidin as a regenerable biorecognition element on boron-doped diamond for biotin sensing

Buzid, Alyah; Hayes, Phyllis E.; Glennon, Jeremy D.; Luong, John H. T.

doi:10.1016/j.aca.2019.01.058

Cited by 19 publications

(11 citation statements)

References 33 publications

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“…Anticancer drugs (doxorubicin) [72,95,96] Anticancer and other drugs [16] Anticancer drugs (doxorubicin) [97] Carbon quantum dots: Anticancer drugs (temozolomide) [28] (Bio)sensors Electrochemical: cholesterol [98]; glucose and bacteria [110]; avian leucosis virus [111]; organic liquids [112] Electrochemical: ATP metabolites [102]; oxygen [84] Electrochemical: Biotin [66] Carbon black: Electrochemical aptasensor for S. aureus [32]; electrochemical sensor for H 2 O 2 [33] Piezoelectric: strain, human motion [63,99,113] Piezoresistance and thermoelectric-based: pressure and temperature [103]; pressure [17]; strain, human motion [90,91,114];…”

Section: Drug Deliverymentioning

confidence: 99%

“…For example, a sensor for biotin was developed by the adsorption of captavidin, a nitrated avidin with moderate affinity to biotin, on a carboxymethylcellulose layer stabilized on a boron-doped diamond electrode by a Nafion film. This biosensor was used for analyzing biotin in blood plasma [66] (Table 1).…”

Section: Preparation and (Bio)application Of Nanocellulose/nanodiamonmentioning

confidence: 99%

“…In addition, nanocellulose/nanocarbon composites are promising for biomedical applications, though these applications are less frequent than industrial applications. Biomedical applications include radical scavenging [34,92], photothermal ablation of pathogenic bacteria [93], photodynamic and combined chemophotothermal therapy against cancer [35,94], drug delivery [16,28,65,72,[95][96][97], biosensorics [31][32][33]63,66,71,91,[98][99][100][101][102][103][104], and particularly tissue engineering and wound dressings. Hybrid materials containing nanocellulose and nanocarbons stimulated the growth and osteogenic differentiation of human bone marrow mesenchymal stem cells [37,59].…”

Section: Introductionmentioning

confidence: 99%

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Applications of Nanocellulose/Nanocarbon Composites: Focus on Biotechnology and Medicine

et al. 2020

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Nanocellulose/nanocarbon composites are newly emerging smart hybrid materials containing cellulose nanoparticles, such as nanofibrils and nanocrystals, and carbon nanoparticles, such as “classical” carbon allotropes (fullerenes, graphene, nanotubes and nanodiamonds), or other carbon nanostructures (carbon nanofibers, carbon quantum dots, activated carbon and carbon black). The nanocellulose component acts as a dispersing agent and homogeneously distributes the carbon nanoparticles in an aqueous environment. Nanocellulose/nanocarbon composites can be prepared with many advantageous properties, such as high mechanical strength, flexibility, stretchability, tunable thermal and electrical conductivity, tunable optical transparency, photodynamic and photothermal activity, nanoporous character and high adsorption capacity. They are therefore promising for a wide range of industrial applications, such as energy generation, storage and conversion, water purification, food packaging, construction of fire retardants and shape memory devices. They also hold great promise for biomedical applications, such as radical scavenging, photodynamic and photothermal therapy of tumors and microbial infections, drug delivery, biosensorics, isolation of various biomolecules, electrical stimulation of damaged tissues (e.g., cardiac, neural), neural and bone tissue engineering, engineering of blood vessels and advanced wound dressing, e.g., with antimicrobial and antitumor activity. However, the potential cytotoxicity and immunogenicity of the composites and their components must also be taken into account.

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Section: Drug Deliverymentioning

confidence: 99%

Section: Preparation and (Bio)application Of Nanocellulose/nanodiamonmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Applications of Nanocellulose/Nanocarbon Composites: Focus on Biotechnology and Medicine

et al. 2020

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“…To date, a number of studies have reported on the utilization of avidin-biotin interaction in sensing technology [46][47][48][49][50]. The detection limits ranged from 2 pM to 84 nM have been reported using various detection methods such as competitive immunoassay, electrochemical, cyclic voltammetry, and immunoaffinity chromatography as listed in Table 5.1.…”

Section: Comparative Sensitivity and Practical Applicabilitymentioning

confidence: 99%

Graphene FET Biosensor Based on the Avidin–Biotin Technology

Wang

Hossain

Zhao

et al. 2021

Graphene Field-Effect Transistor Biosensors

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Avidin-biotin interaction is the strongest known protein-ligand interaction and is widely used in the field of biomedicine, including immunochromatography and various biological detection technologies. Because avidin or streptavidin can specifically bind to four biotinylated molecules efficiently and quickly, and the biotinylated molecules will not change their inherent characteristics. At present, this avidin-biotin interaction has been widely used in the field of biomedical detection to amplify biological signals for specific molecules. Combined with the graphene fieldeffect transistor biosensor, using the avidin-biotin interaction, the concentration of specific molecules in the test solution is expected to be amplified by the biotin-avidin interaction, which helps to increase the limit of detection for the biosensor. Besides, based on the avidin-biotin interaction, the graphene field-effect transistor biosensor is expected to rapidly quantitative detect free biotin and biotinylated molecules. This chapter mainly introduces the graphene field-effect transistor biosensor from the perspectives of device preparation, surface modification, sensitivity, and specificity, and discusses the possible situations that the sensor may encounter in actual detection.Avidin-biotin technology is widely used in different types of ELISA (enzyme-linked immunosorbent assay) kits, polymer-based detection and labeled immunosensor s for the detection of different bio-markers linked to different diseases such as cancer and influenza. In this section, we introduced the employing avidin-biotin technology in the graphene FET biosensor and demonstrated the specific detection of the biotinylated biomolecule in the sub-pico molar (pM) range. The sensing performance of graphene FET biosensor was characterized by the real-time two-terminal electrical current measurement upon injection of analyte solution into a silicone pool preattached onto the graphene channel. Since the Avidin-biotin technology has strong affinity and specificity, any biotinylated biomolecules are hopping for rapid detecting with ultra-low concentration level through this sensing platform. Thus the present graphene FET biosensor is expected to be a breakthrough in biomedical analysis. It can be used as a potential common platform for the rapid and point of care detection of different biomolecules and biomarkers linked to different diseases.

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“…Nevertheless, it is feasible to oxidize biotin by boron-doped diamond as electrode materials 22 and the use of captavidin with low affinity to streptavidin toward the development of a reusable sensor for biotin in electrochemistry. 23…”

Section: Detection Of Biotinmentioning

confidence: 99%

Chemistry of Biotin–Streptavidin and the Growing Concern of an Emerging Biotin Interference in Clinical Immunoassays

Luong

Vashist

2019

ACS Omega

Self Cite

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Overconsumption of biotin (5−100 mg daily) as a supplement by the general population poses a significant problem for clinical immunoassays (IAs) based on biotin−streptavidin (SA) interactions. This affinity pair has been exploited in immunoassays because of its avidity, sensitivity, specificity, and stability. The elevated biotin level in plasma varies from patient to patient, and its severe interference cannot easily be predicted and quantified. Thus, immunoassay manufacturers must investigate the biotin interference in the developed immunoassays to satisfy the threshold of 3510 ng/mL (14 367 nM), as stipulated by the FDA. There is no concrete solution to circumvent the biotin interference without extra costs and technical difficulties, albeit different strategies have been attempted. They include the IA format with biotinylated reagents prebound to streptavidin, the removal of biotin from the specimen, sample treatment, and biotin interference-free assays. The general public has been instructed to stop taking biotin supplements for 48 h or even weeks before the test, depending on the specific test, dose, and frequency of biotin uptake. As lab-based techniques cannot accommodate an enormous number of public samples, a rapid analytical procedure for biotin is urgently needed to quantify for its interference in immunoassays.

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Captavidin as a regenerable biorecognition element on boron-doped diamond for biotin sensing

Cited by 19 publications

References 33 publications

Applications of Nanocellulose/Nanocarbon Composites: Focus on Biotechnology and Medicine

Applications of Nanocellulose/Nanocarbon Composites: Focus on Biotechnology and Medicine

Graphene FET Biosensor Based on the Avidin–Biotin Technology

Chemistry of Biotin–Streptavidin and the Growing Concern of an Emerging Biotin Interference in Clinical Immunoassays

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