2016
DOI: 10.1039/c6tb00606j
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Capturing red blood cells from the blood by lectin recognition on a glycopolymer-patterned surface

Abstract: A newly glycopolymer-patterned surface for capturing red blood cells (RBCs) is demonstrated. Our strategy is based on the surface-initiated photopolymerization of 2-acryl-amido-2-methylpropane sulfonic acid (AMPS) on a thermoplastic elastomer, the patterning of poly(D-gluconamidoethyl methacrylate) (PGAMA, glycopolymer) micro-domains on the PAMPS layer with photomask-assisted photolithography, followed by the generation of a phytohemagglutinin (PHA) array on the patterned surface through lectin-carbohydrate re… Show more

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Cited by 14 publications
(5 citation statements)
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“…After methacrylation, the peak at 1612 cm –1 shifts to 1645 cm –1 due to an amide bond (amide I) formation between aminoethyl methacrylate and carboxylic acid/methyl carboxylate groups, and the intensity of the peak at 1645 cm –1 is increased with increasing degree of methacrylation. In particular, the peak at 1645 cm –1 stems from amide I, while the peak at 1544 cm –1 corresponds to amide II and that at 806 cm –1 to CH acrylate groups. After UV-cross-linking, the amide I peak from PEMA shifts to 1600 cm –1 and the acrylate (CH)-derived signal disappears. , Furthermore, FTIR spectroscopy was also used to characterize the interaction of Gelin-S after UV-cross-linking with PEMA. From these spectra, we observed a thiol-Michael addition reaction between thiolated gelatin (Gelin-S) and the methacrylate groups present on PEMA, which is evident from the disappearance of the acrylate associated at 806 cm –1 and the thiol group at 2450 cm –1 due to the reaction between thiol and the acrylate groups (Figure S1b).…”
Section: Resultsmentioning
confidence: 98%
“…After methacrylation, the peak at 1612 cm –1 shifts to 1645 cm –1 due to an amide bond (amide I) formation between aminoethyl methacrylate and carboxylic acid/methyl carboxylate groups, and the intensity of the peak at 1645 cm –1 is increased with increasing degree of methacrylation. In particular, the peak at 1645 cm –1 stems from amide I, while the peak at 1544 cm –1 corresponds to amide II and that at 806 cm –1 to CH acrylate groups. After UV-cross-linking, the amide I peak from PEMA shifts to 1600 cm –1 and the acrylate (CH)-derived signal disappears. , Furthermore, FTIR spectroscopy was also used to characterize the interaction of Gelin-S after UV-cross-linking with PEMA. From these spectra, we observed a thiol-Michael addition reaction between thiolated gelatin (Gelin-S) and the methacrylate groups present on PEMA, which is evident from the disappearance of the acrylate associated at 806 cm –1 and the thiol group at 2450 cm –1 due to the reaction between thiol and the acrylate groups (Figure S1b).…”
Section: Resultsmentioning
confidence: 98%
“…However, to date, there has been very little work focused on fabricating synthetic glycopolymer‐based materials, and the main focus of available studies has been to exploit the properties of the sugar–lectin interactions as a mechanism to isolate or selectively culture a specific cell type from a heterogeneous population or to facilitate enzyme immobilization for delivery purpose . Here for the first time, we report the fabrication of aligned glycopolymer‐based electrospun membranes, investigating their mechanical behavior in detail, and presenting that they can successfully provide cell guidance over tenocytes.…”
Section: Resultsmentioning
confidence: 99%
“…This approach can be applied for the design of new drug delivery systems, especially focused in immunotherapies. Besides specific interaction with lectins, surfaces modified with glycopolymers can also present other properties, such as antifouling, antimicrobial, or antiviral activity [17,44,49,[67][68][69]. Despite the relevance of these applications, the examples reported in the literature using glycopolymer brushes are scarce compared with the ones dealing with linear glycopolymers [9].…”
Section: Glucose-containing Polymer Brushesmentioning
confidence: 99%
“…Fouling of membranes is common in biomedical applications and, although not fully understood, it is known that this phenomenon is associated with protein adsorption at the surface of the material. For example, a poly(vinylidene difluoride) (PVDF) microporous membrane was modified with PGAMA by aqueous SI-activator Besides specific interaction with lectins, surfaces modified with glycopolymers can also present other properties, such as antifouling, antimicrobial, or antiviral activity [17,44,49,[67][68][69]. Despite the relevance of these applications, the examples reported in the literature using glycopolymer brushes are scarce compared with the ones dealing with linear glycopolymers [9].…”
Section: Glucose-containing Polymer Brushesmentioning
confidence: 99%