Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging

Salamon, Rebecca J.; Zhang, Ziheng; Mahmoud, Ahmed I.

doi:10.3791/60482

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…Our passive CLARITY technique was performed with minimal modifications 36 in hearts that required imaging of structures deep within the tissue (i.e. coronary arteries).…”

Section: Methodsmentioning

confidence: 99%

Defining Cardiac Nerve Architecture During Development, Disease, and Regeneration

Salamon

Halbe

Kasberg

et al. 2023

Preprint

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Cardiac nerves regulate neonatal mouse heart regeneration and are susceptible to pathological remodeling following adult injury. Understanding cardiac nerve remodeling can lead to new strategies to promote cardiac repair. Our current understanding of cardiac nerve architecture has been limited to two-dimensional analysis. Here, we use genetic models, whole-mount imaging, and three-dimensional modeling tools to define cardiac nerve architecture and neurovascular association during development, disease, and regeneration. Our results demonstrate that cardiac nerves sequentially associate with coronary veins and arteries during development. Remarkably, our results reveal that parasympathetic nerves densely innervate the ventricles. Furthermore, parasympathetic and sympathetic nerves develop synchronously and are intertwined throughout the ventricles. Importantly, the regenerating myocardium reestablishes physiological innervation, in stark contrast to the non-regenerating heart. Mechanistically, reinnervation during regeneration is dependent on collateral artery formation. Our results reveal how defining cardiac nerve remodeling during homeostasis, disease, and regeneration can identify new therapies for cardiac disease.

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“…Our passive CLARITY technique was performed with minimal modifications 36 in hearts that required imaging of structures deep within the tissue (i.e. coronary arteries).…”

Section: Methodsmentioning

confidence: 99%

Defining Cardiac Nerve Architecture During Development, Disease, and Regeneration

Salamon

Halbe

Kasberg

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our passive CLARITY technique was performed with minimal modifications 59 in hearts that required imaging of structures deep within the tissue (i.e., coronary arteries). Briefly, hearts were harvested, washed in PBS, and placed into 4% PFA, incubating overnight at 4°C with rotation.…”

Section: Methodsmentioning

confidence: 99%

Parasympathetic and sympathetic axons are bundled in the cardiac ventricles and undergo physiological reinnervation during heart regeneration

Salamon,

Halbe,

Kasberg

et al. 2023

iScience

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Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging

Cited by 2 publications

References 26 publications

Defining Cardiac Nerve Architecture During Development, Disease, and Regeneration

Defining Cardiac Nerve Architecture During Development, Disease, and Regeneration

Parasympathetic and sympathetic axons are bundled in the cardiac ventricles and undergo physiological reinnervation during heart regeneration

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